Human APOA1/Apolipoprotein A1 protein participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase. Defects in APOA1/Apolipoprotein A 1 are a cause of familial hypoalphalipoproteinemia and the low HDL levels as well as amyloidosis van Allen type disease.

In this ELISA kit, an APOA1/Apolipoprotein A1 specific antibody has been pre-coated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an APOA1/Apolipoprotein A1 specific biotinylated detection antibody is added and followed by washing with wash buffer. Afterwards, Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of APOA1/Apolipoprotein A1 captured in the plate.