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Invitrogen™Cells-to-CT™ 1-Step Power SYBR™ Green Kit

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Makes it possible to perform expression analysis directly from cultured cells without RNA purification

Supplier:  Invitrogen™ A25601

Dimensions 10 × 10 × 10 in
Size/Quantity

Brand

SKU: A25601 Category:

Description

The one-step format of RT-PCR step streamlines the Cells-to- CT “sample to answer” workflow more than ever without compromising sensitivity and specificity and at extraordinary value.

 

  • Complete: optimized workflow includes cell lysis reagents with gDNA removal and Power SYBR Green 1-Step RT-PCR Master Mix
    Faster: 7-minute sample prep, including DNase treatment, versus 30-60 minutes for traditional RNA purification
  • Simple: 12 steps total (sample prep and real-time RT-PCR combined)
  • Robust: Perform gene expression analysis on 10 – 100,000 cells per sample; results equivalent to those from purified RNA
  • Study multiple targets per sample at an affordable price

Specifications

• 2.2 mL Lysis Solution, store at 2–8°C
• 22 µL Dnase I, store at –5 to –30°C
• 200 µL Stop Solution, store at –5 to –30°C
• 500 µL Power SYBR™ Green 1-Step qPCR Mix, store at –5 to –30°C
• 10 µL Power SYBR™ Green 1-Step RT Mix, store at –5 to –30°C
Tube
1-step RT-qPCR
7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Cells-to-CT™, Power SYBR™ Green
20 Preps
Cell Lysis
2.2 mL
SYBR
High
Standard
Fewer resources used and less waste, less hazardous
1-Step qPCR kit
Gene Expression
Cell, RNA
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