Description
ITS Premix will stimulate cell proliferation while decreasing substantially the serum requirements for culture of many cell types as diverse, for example, as contractile rat heart cells and human colon mucosal epithelial cells. Basal media supplemented with ITS Premix and as little as 2% Fetal Bovine Serum support proliferation of many diploid and heteroploid cell lines at rates equivalent to those obtained with 10% serum. Contains 0.5 mg/ml insulin from bovine pancreas, 0.5 mg/ml human transferrin (substantially iron-free) and 0.5 ug/ml sodium selenite. Prepared in Earle’s balanced Salt Solution (EBSS) without phenol red. Sterile-filtered.