Description
• Intended Use: Lowenstein – Jensen Medium is used with fresh egg and glycerol for the isolation and differentiation of Mycobacterium spp.
• Product Summary and Explanation:
Mycobacterial infections, particularly tuberculosis, are a worldwide health problem. Almost three million people worldwide die of tuberculosis each year. Non-tuberculous mycobacteria infections have also increased since 1985. At least 25 species of mycobacteria are associated with human disease and produce usually slowly developing, destructive granulomas that may undergo necrosis with ulceration or cavitation.
The use of egg-based media for primary isolation of mycobacteria have two significant advantages. First, egg-based media support a wide variety of mycobacteria. Second, growth of mycobacteria on egg media can be used for niacin testing. Liquefaction of Lowenstein-Jensen Medium can occur if contaminated with proteolytic organisms.
Lowenstein-Jensen Medium is a modification of Lowenstein Medium, modified by Jensen. Jensen modified the medium by alternating the citrate and phosphate contents, eliminating congo red dye, and increasing malachite green concentration. Lowenstein-Jensen Medium is commonly used in the clinical laboratory to isolate acid fast organisms from sterile and nonsterile sources.
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