The MagBeads FastDNA Kit for Microbiome is an advanced genomic DNA (gDNA) extraction solution based on the next-generation silicon-based magnetic bead technology.

This kit is designed to deliver high-fidelity gDNA extraction from a diverse array of biological specimens, encompassing bacterial communities, a spectrum of bodily fluids, bronchoalveolar lavage fluids, and environmental samples such as soil.

The kit integrates an effective lysis mechanism, ensuring thorough cell lysis and subsequent DNA purification across a broad spectrum of microorganisms, including bacteria, archaea, and fungi.

The resulting gDNA is of high purity and suitable for numerous downstream molecular applications, such as Polymerase Chain Reaction (PCR), Quantitative Real-Time PCR (qRT-PCR), and metagenomic library construction, facilitating comprehensive genomic analyses.

Microbial culture samples

Figure 1: The MagBeads FastDNA Kit for Microbiome produced higher yield (μg) of DNA. Comparison of extraction of gDNA from two different strains of bacteria (~109 cfu) using MagBeads FastDNA Kit for Microbiome and competitors kits. Bacteria strains: Escherichia coli (Blue), Staphylococcus aureus (Yellow); M: DNA marker, 15 kbp.

Bronchoalveolar lavage fluid (BALF)

Figure 2: DNA extraction was performed using 3.3 mL of bronchoalveolar lavage fluid (BALF). The MagBeads FastDNA Kit for Microbiome produced the highest DNA yield and purity compared to the competitor kits tested. M: DNA marker, 15 kbp.

Yogurt

Figure 3: MagBeads FastDNA Kit for Microbiome gave high DNA yield and purity for 200 μL yogurt. DNA marker: 15 kbp.

DNA qPCR

Figure 4: Comparison of threshold cycles (Ct) in qPCR amplification using equal quantities of gDNA (30 ng) isolated from yogurt, soil, saliva, and urine with the MagBeads FastDNA Kit for Microbiome and competitor kits. Targets were amplified using SYBR green technology with primers for 16S rRNA (197 bp) and Globin (400 bp).