Description
• Intended Use: Middlebrook 7H11 Agar is used with glycerol and OADC Enrichment for the cultivation of Mycobacterium spp.
• Product Summary and Explanation: There are two types of solid culture media for the primary isolation of mycobacteria, coagulated egg as a base (Lowenstein formulations) and an agar base (Middlebrook formulations). The use of agar-based media for primary isolation of mycobacteria have the following significant advantages:
1. Agar-based media do not usually liquefy in the presence of contaminating proteolytic organisms.
2. Agar-based media are recommended for specimens from nonsterile sites, because colonies of mycobacteria can be viewed in a clear medium after 10 – 12 days incubation using a stereo microscope even if contaminating organisms are present.
3. Agar- based media retain exact concentrations of added drugs because the medium is solidified with agar rather than by inspissation of the egg. There is less drug inactivation when egg ingredients are absent.
Middlebrook 7H11 Agar is a modification of Middlebrook 7H10 Agar Special as recommended by Cohn, Waggoner, and McClately. Cohn et al. added an enzymatic digest of casein and found organism growth was stimulated for fastidious strains of Mycobacterium tuberculosis and provided improved susceptibility testing.
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