Description
Camelid
VHH Antibody (also called single-domain antibody, sdAb or Nanobody by Ablynx)
is a peptide chain about 110 amino acids in length, containing one variable domain
of a heavy-chain antibody (a VHH fragment). Like a whole antibody, the camelid
VHH antibody is able to bind selectively to a specific antigen. This single-domain
antibody has a broad range of applications in biotechnical and therapeutic research
fields due to its small size, simple production and high affinity.
GenScript’s MonoRab™ Anti-Camelid VHH
Affinity Resin (Cat. No. L00905) is designed for purification of Camelid VHH
single domain antibody from commonly used protein expression systems such as bacteria,
yeast and mammalian cells. The VHH single domain antibody in cell lysate can specifically
bind to the Anti-Camelid VHH monoclonal antibody coupled to the resin. Nonspecific
binding reactions are eliminated by stringent washing steps, and the purified Camelid
VHH single domain antibody is eluted with a high recovery rate. Table
1 lists the main characteristics of GenScript’s MonoRab™ Anti-Camelid VHH
Affinity Resin.
Table 1. Characteristics of Anti-Camelid VHH Affinity
Resin
| Product content | 50% settled resin in TBS with 0.02% sodium azide |
| Matrix | 4% cross-linked agarose |
| Average bead size | 90 μm |
| Ligand | MonoRab™ Rabbit Anti-Camelid VHH Antibody, mAb |
| Binding capacity | Approximately 2-4 mg Camelid VHH protein per mL settled resin (depending on amino acid sequence of different Camelid VHHs) |
| Storage and stability | Store at 2-8 °C for up to 12 months. Do not freeze the resin. |
| Resin reuse | When stored at 2 – 8° C, the resin can be recycled at least 5 times with no loss of binding capability. The resin can be reused up to 10 times with minimum loss of binding capability. |
| Elution method | Acid buffer elution (pH 2.5) |
| Reagents compatibility | Compatible with commonly used bacterial lysis reagents. For reagents compatible with purification, see tables 2 and 3 in the manual. |