Description
Multiple Tag is a specially designed fusion protein that can serve as an excellent positive control for western and dot blots. The fusion protein has an apparent molecular weight of ~40 kDa on SDS-PAGE and it contains 16 commonly used tags from N terminus to C terminus in the order as shown in the table below.
Tag | Sequence |
---|---|
Trx | MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLA |
T7 | CMASMTGGQQMG |
HSV | QPELAPEDPEDC |
c-Myc | EQKLISEEDLC |
VSV-g | YTDIEMNRLGK |
Glu-Glu | CEEEEYMPME |
V5 | GKPIPNPLLGLDST |
E-tag | GAPVPYPDPLEPR |
Flag-tag | DYKDDDDK |
S-tag | KETAAAKFERQHMDS |
HA | YPYDVPDYAC |
KT3 | KPPTPPPEPET |
E2 | GVSSTSSDFRDR |
AU1 | DTYRYI |
AU5 | TDFYLK |
6x His | HHHHHH |