Description
Multiple Tag is a specially designed fusion protein that can serve as an excellent positive control for western and dot blots. The fusion protein has an apparent molecular weight of ~40 kDa on SDS-PAGE and it contains 16 commonly used tags from N terminus to C terminus in the order as shown in the table below.
| Tag | Sequence |
|---|---|
| Trx | MSDKIIHLTD DSFDTDVLKA DGAILVDFWA EWCGPCKMIA PILDEIADEY QGKLTVAKLN IDQNPGTAPK YGIRGIPTLL LFKNGEVAAT KVGALSKGQL KEFLDANLA |
| T7 | CMASMTGGQQMG |
| HSV | QPELAPEDPEDC |
| c-Myc | EQKLISEEDLC |
| VSV-g | YTDIEMNRLGK |
| Glu-Glu | CEEEEYMPME |
| V5 | GKPIPNPLLGLDST |
| E-tag | GAPVPYPDPLEPR |
| Flag-tag | DYKDDDDK |
| S-tag | KETAAAKFERQHMDS |
| HA | YPYDVPDYAC |
| KT3 | KPPTPPPEPET |
| E2 | GVSSTSSDFRDR |
| AU1 | DTYRYI |
| AU5 | TDFYLK |
| 6x His | HHHHHH |


