Description
Mycoplasmas are commonly found in research laboratories and unaffected by many of the antibiotics that have been used to control bacterial contamination in cell cultures, especially those that affect cell wall formation, such as penicillin. The lack of a cell wall also means that some cells may be able to pass through 0.2 μm filters. There have been several studies to determine the prevalence of mycoplasma contamination in cell cultures. Some have estimated that it could be as high as 80%, while studies in the USA suggest a lower contamination rate of 10-15%. An average figure between 25% and 50% worldwide seems quite plausible. This is a very significant problem for the industry and could lead to serious economic losses. Most cases of cell culture contamination are caused by a group of about six to eight species with human, porcine, or bovine hosts, of which Mycoplasma orale and Mycoplasma hyorrhinis are the most common.
Myco-Visible Mycoplasma LAMP detection kit is a highly sensitive, specific, and rapid assay intended for the detection of mycoplasma contaminations in cell cultures. Myco-Visible Mycoplasma LAMP detection kit achieves this by targeting and amplifying the 16S rRNA coding region of the mycoplasma genome at loci that are well conserved in all Mollicutes, including Acholeplasma laidlawii, Mycoplasma arginini, Mycoplasma fermentans, Mycoplasma hominis, Mycoplasma hyorhinis, and Mycoplasma orale. The detection procedure can be completed within 45 mins and requires as little as 10 16S rRNA copies or 10 fg of Mycoplasma genomic DNA per reaction. No cross-reactivity with other bacterial, fungal and mammalian DNA by Primer-BLAST. The test assay is easy to perform with no thermal cycler required. Positive results can be visualized by color changes from pink to yellow.
Features
No Special Instrument Required: Direct detection with just a dry bath
Rapid and Comprehensive: Detects Mycoplasma spp in 45 mins
Sensitive: Requires as little as 10 16S rRNA copies or 10 fg of Mycoplasma genomic DNA per reaction
Easy to Use: Innovative colorimetric detection system – vivid color change
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Results Interpretation
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Test result shall be interpreted as positive if the reaction solution turns yellow or orange.
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Test result shall be interpreted as negative if the reaction solution remains as pink.
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Figure 1: (Left) Color of reaction solution turns yellow or orange, interpret the result as positive. (Right) Result is interpreted as negative if the solution remains in pink color.
Performance
Figure 2: Analytical sensitivity test of Myco-Visible Mycoplasma LAMP Detection Kit. 10-fold serial dilution from 10 to 103 copies/µl was performed using six strains of Mycoplasma spp. Results showed that the kit could detect as low as 10 16S rRNA copies or 10 fg of Mycoplasma genomic DNA.