Neurobasal™-A Medium

Neurobasal™-A Medium is a basal medium formulated to meet the special cell culture requirements of post-natal and adult brain neuronal cells when used with GIBCO™ B-27™ Supplement. Neurobasal™-A Medium can be used to grow neuronal cells from hippocampus, cortex and other regions of the brain. Neurobasal™-A Medium allows for both long and short term maintenance of homogeneous populations of neuronal cells without the need of an astrocyte feeder layer.

Add Your Own Supplements
All Neurobasal™ media contain no L-glutamine, L-glutamic acid, or aspartic acid. Neurobasal™ media must be supplemented with 0.5 mM L-glutamine or GlutaMAX™-I Supplement and either a serum-free supplement (such as B-27™ Supplement or N-2 Supplement), or with serum. For initial plating, you should add 25µM glutamic acid.

Quality Control Testing
All Neurobasal™ media are tested for pH, osmolality, endotoxin, and the absence of bacterial and fungal contamination. The osmolality of Neurobasal™-A was demonstrated to be optimal for postnatal and adult CNS neurons.

Neurobasal™-A Medium contains Phenol Red. If Phenol Red will interfere with your applications, we also provide Neurobasal™-A Medium Minus Phenol Red.

Neurobasal™-A Medium is optimized for post-natal and adult brain neurons. For growing pre-natal and embryonic neurons, we provide Neurobasal™ Medium.