Description
GenScript Protein L Resin is an affinity chromatography medium designed
for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The highly purified protein L ligand is
coupled to 4% agarose. The coupling is
optimized to give high binding capacity for immunoglobulins.
The static binding capacity of Protein L Resin is greater than 15 mg rabbit IgG/ml settled resin. The dynamic binding capacity will vary depending
on several factors such as target antibody, flow rate etc.
Protein L, first isolated from the surface of
bacterial species Peptostreptoccus magnus,
binds immunoglobulin through κ light chain interaction. Protein L binds a wider
range of Ig classes and subclasses than other antibody-binding proteins such as
protein A or protein G. It can bind to all classes of Ig (i.e., IgG, IgM, IgA,
IgE, and IgD) and also the single chain variable fragments (Scfv) and Fab
fragments.