Description
Size quantity : 1-EA
Description : The Rapid Plus DNA Lib Prep kit for Illumina® V2 provides a streamlined and efficient method to construct DNA libraries for next generation sequencing (NGS) on Illumina® sequencing platforms. This kit is an updated version of the original Rapid Plus DNA Lib Prep kit (Cat. Nos. RK20208) that has been further optimized to provide superior DNA damage repair and improve ligation efficiency with low-input DNA. The entire protocol can be completed within 2 hours. Starting from fragmented input-DNA (1 – 1000 ng), the kit contains all of the necessary enzymes and buffers for end-preparation, adapter ligation and library amplification. To simplify the workflow, fragmented DNA is end-repaired and adenylated during one initial step. The kit can be conveniently used for construction of PCR-free libraries beginning with 100 ng of high-quality fragmented genomic DNA, or from 10 ng of circulating cell-free DNA (cfDNA). PCR primers provided in the kit are optional for users who prefer self-selected high-fidelity DNA polymerases in library amplification.The kit also provides DNA repair enzymes which repair DNA damage such as nicks, gaps, oxidized bases, damaged/blocked 3’ ends, AP sites (apurinic/apyrimidinic sites), and uracil bases. In addition, the DNA repair enzymes significantly increase NGS library yields from FFPE-derived DNA.