RPMI 1640 Medium

Roswell Park Memorial Institute (RPMI) 1640 Medium is suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

• • RPMI is modified as follows: with L-glutamine, phenol red; without HEPES

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• • Unique from other media because it contains reducing agent glutathione and high concentrations of vitamins

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• • Contains biotin, vitamin B₁₂, and PABA, which are not found in Eagle’s Minimal Essential Medium or Dulbecco’s Modified Eagle Medium

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• • Vitamins inositol and choline are present in high concentrations

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• • Contains no proteins, lipids, or growth factors

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• • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)

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• • Uses sodium bicarbonate buffer system (2.0g/L), and therefore requires a 5-10% CO₂ environment to maintain physiological pH

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• • Sustainable packaging

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Cell Culture, Mammalian Cell Culture