Description
SPINeasy DNA Kit for Microbiome allows for efficient and unbiased lysis of microbes, including gram positive/negative bacteria, fungi, protozoans, and viruses found in various swab, body fluid, stool, soil, and water samples. This kit features a highly effective lysis system and silica membrane spin column technology. With the use of specially formulated Buffer MB1 and Lysing Matrix E in combination with FastPrep instruments from MP Biomedicals, efficient lysis of various samples can be achieved within seconds. Provided with the kit, Column MB and kit buffers are designed to deliver gDNA of high yield and purity in fewer steps, ready for downstream applications such as qPCR, restriction digestion, and sequencing.
Performance Data
SPINeasy DNA Kit for Microbiome has been rigorously tested for its performance using a variety of samples. The following are a few examples of the results of gDNA extraction using SPINeasy DNA Kit for Microbiome. The concentration of the extracted DNA was determined by a fluorometric method, and quality was evaluated using agarose gel electrophoresis and/or qPCR.
Bronchoalveolar lavage fluid (BALF)
The DNA extracted with SPINeasy DNA Kit for Microbiome demonstrated high yield and good DNA integrity.
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Results: DNA was extracted from 1 mL BALF using SPINeasy DNA Kit for Microbiome and other competitor kits. The results from the tree replicates were consistent.
Human whole blood
Microbial DNA extracted with SPINeasy DNA Kit for Microbiome showed the highest yields and purity compared to competitor kits. Extractions were performed using 200 µL of human whole blood spiked with a bacterial solution.
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Results: 200 µL of human whole blood (spiked with 10 µL of bacterial solution) were added directly to Lysing Matrix E, and used for DNA extraction. SPINeasy DNA Kit for Microbiome gave the highest yield and good DNA integrity as compared to the competitor kits. The results from the three replicates were consistent; 15,000 bp