SPINeasy DNA Kit for Water is a high-performance water genomic DNA extraction kit based on silica membrane spin column technology. The kit has been specifically designed to concentrate microbes present in water samples by filtration and transfer to a Lysing Matrix tube (4.5 mL) for the best extraction of microbial gDNA from water in less than 30 min. Water samples are processed using our uniquely formulated Lysis Buffer W1 and Lysing Matrix E to effectively lyse various types of microbial cells, including bacteria, fungi, virus, and protozoans. Column W1 (provided with the kit) has high binding capacity and selectivity for gDNA. The combination of components in the kit helps extract microbial gDNA of high yield and purity that is ready for downstream applications, such as restriction digestion, PCR, and NGS.

Performance Data

SPINeasy DNA Kit for Water has consistently achieved better yield along with high purity as compared to competitors’ kits. Total yield is calculated by multiplying the DNA concentration by total purified sample volume, and integrity is determined by absence of visible shearing or smearing of DNA bands during gel electrophoresis.

Results: SPINeasy DNA Kit for Water consistently achieved better yield across various types of water samples as compared to competitors’ DNA kits.

SPINeasy DNA Kit for Water produced more intact DNA bands in extracted samples compared to competitor DNA kits

M: 1kb plus DNA ladder
1: River Water
2: Pond Water
3: Sea water
4: Sewage
M: SPINeasy DNA Kit for Water from MP Biomedicals
Q: Competitor 1 Kit
O: Competitor 2 Kit

Results: SPINeasy DNA Kit for Water resulted in more intact bands of extracted DNA as compared to competitors’ DNA kits.