Description
SPINeasy DNA/RNA/Protein All-In-One Kit utilizes a convenient workflow and silica membrane spin columns to isolate DNA, RNA, and protein components from the same sample, without the use of toxic substances such as phenol and chloroform. The use of our specially formulated Lysis Buffer R and Lysing Matrix A in combination with FastPrep instruments from MP Biomedicals enables highly efficient lysis of tissue samples within seconds. DNA, RNA, and proteins are then sequentially purified from the same lysate. Briefly, DNA is absorbed onto the first spin column, while the flow-through is collected and used for RNA purification. The second spin column captures RNA and the flow-through from this step is used for protein extraction. DNA and RNA are bound on the first and second columns, respectively, and then washed and eluted. In the final extraction, proteins are precipitated out of solution, pelleted down by centrifugation, washed, and resuspended. Each molecular component is then immediately available for their respective downstream applications.
Performance Data
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M: DNA marker |
Figure 1: (A) DNA; (B) RNA; (C) protein extracted from each animal tissue using SPINeasy DNA/RNA/Protein All-In-One Kit, analyzed using gel electrophoresis.
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M: DNA marker |
Figure 2: (A) PCR amplification of DNA and (B) RT-PCR amplification of RNA extracted from various samples using SPINeasy DNA/RNA/Protein All-In-One Kit.