Description
The SPINeasy Host Depletion Microbial DNA Kit provides an easy-to-use workflow to isolate and enrich microbial DNA from samples containing high amounts of host DNA. Host DNA contamination is common in samples such as swabs and bodily fluids. The presence of high amounts of human DNA can limit the application and sensitivity of downstream shotgun metagenomic sequencing or microbiome analysis, as most of the extracted DNA is from human and not microbial origin.
The SPINeasy Host Depletion Microbial DNA Kit provides efficient reduction of host DNA from these samples by selective lysis of host cells with our specially formulated Host Lysis Buffer. Host DNA, which has been released into the solution, is then removed from the supernatant through centrifugation. A further host depletion treatment provides enzymatic degradation of any remaining host DNA, as well as DNA coming from dead microbial cells. Finally, the remaining intact microbial cells are lysed through a combination of specially formulated buffers and highly efficient homogenization using FastPrep instruments from MP Biomedicals. Enriched microbial DNA is then purified using a convenient silica membrane spin-column technology workflow and ready for downstream molecular applications, such as PCR and NGS.
Performance Data
The following are PCR results of DNA extraction from saliva samples using SPINeasy Host Depletion Microbial DNA Kit, showing effective host DNA depletion and microbial DNA recovery. Both host DNA depletion and bacteria DNA recovery are estimated to be higher than 90% for these samples, using qPCR analyses.
 | M: DNA marker |
Figure 1: PCR of DNA extracted from saliva samples using SPINeasy Host Depletion Microbial DNA Kit. (A) PCR detection of host DNA using human β-globin primers. (B) PCR detection of bacterial DNA using 16S primers.