SPINeasy PCR Purification & Gel Extraction Kit

Description

The SPINeasy PCR Purification and Gel Extraction Kit is a silica -membrane spin -column kit that enables quick and convenient DNA clean up from various enzymatic reactions, such as PCR and restriction digestion, as well as isolation and purification of DNA fragments from agarose gels. Up to 23 µg of DNA of molecular weight ranging from 100 bp to 20 kb can be purified through a quick and simple process from most grades of agarose gels with expected recovery of 85%. Purified DNA is immediately ready for routine molecular biology applications.

Performance Data

The following are results of DNA cleaned up using SPINeasy PCR Purification and Gel Extraction Kit. Purified DNA is ready for downstream molecular biology applications.

M: DNA marker Lane 1: Unpurified PCR reaction (input) Lane 2: Purified PCR product Lane 3: Unpurified plasmid restriction digest reaction (input) Lane 4: Purified restriction digested plasmid

Figure 1. Agarose gel electrophoresis of DNA purified using SPINeasy PCR Purification and Gel Extraction Kit. Lanes 1-2 PCR product before and after purification with the kit. Primer-dimers, excess nucleotides, and primers are efficiently removed. Lanes 3-4:linearized plasmid after restriction digestion, before and after gel-extraction using the kit. The majority of DNA recovered after the clean-up process is free of enzymes, salts, and buffers.