Description
SPINeasy Plasmid Miniprep Kit is a silica membrane spin column kit that enables the purification of up to 20 µg of plasmid DNA from 1 – 5 mL of transformed bacterial cultures. High-purity plasmid DNA can be obtained within 25 minutes through a simple purification process, immediately ready for routine molecular biology applications including cloning, restriction digestion, PCR, and in-vitro transcription.
Performance Data
Agarose gel electrophoresis, yield, A260/A280 , and A260/A230 ratios of plasmids extracted using SPINeasy Plasmid Miniprep Kit. Plasmids pWPI and pcDNA6 were isolated from 3 mL DH5α cultures harboring these plasmids.
 | M: DNA Marker |
| Vector Backbone | Plasmid (bp) | Extraction results | ||
| Yield(µg) | A(260/280) | A(260/230) | ||
| pWPI | 11,103 | 14.36 | 1.89 | 2.18 |
| pcDNA6 | 5,149 | 11.24 | 1.86 | 2.19 |