SPINeasy RNA Kit for Tissue is a silica membrane spin column kit that enables quick and convenient purification of total RNA from a variety of tissue samples including cultured cells, fungi, animal tissues, and plant tissues. The use of our specially formulated Lysis Buffer R and Lysing Matrix A in combination with FastPrep® instruments from MP Biomedicals enables highly efficient lysis of tissue samples within seconds. With a simple workflow, the kit allows multiple samples to be processed simultaneously, without the use of toxic substances such as phenol and chloroform. Purified RNA is of high quality and integrity and suitable for RT-PCR and other downstream applications.

Performance Data

Figure 1: RNA extracted from various samples using SPINeasy RNA Kit for Tissue, analyzed using agarose gel electrophoresis. M: DNA marker; Lane 1: Liver; Lane 2: Spleen; Lane 3: Kidney; Lane 4: Lung; Lane 5: Heart; Lane 6: Ginkgo biloba leaf; Lane 7: Prunus davidiana leaf; Lane 8: Cherry leaf; Lane 9: Begonia leaf; Lane 10: Tomato leaf; Lane 11: Peanut leaf; Lane 12: Potato leaf.

Figure 2: RT-PCR amplification of RNA extracted from various samples using SPINeasy RNA Kit for Tissue. M: DNA marker; Lane 1: Liver; Lane 2: Spleen; Lane 3: Kidney; Lane 4: Heart; Lane 5: Lung; Lane 6: Negative control (rabbit β-actin); Lane 7: Ginkgo biloba leaf; Lane 8: Prunus davidiana leaf; Lane 9: Cherry leaf; Lane 10: Begonia leaf; Lane 11: Tomato leaf; Lane 12: Peanut leaf; Lane 13: Potato leaf; Lane 14: Negative control (plant 18S).