Figure 1. Total RNA from Yeast (S. cerevisiae and P. pastoris) was purified using the MP SPINeasy® RNA Kit for Yeast. Extraction was done by both FastPrep® and vortex method. The extracted samples were compared with competitor X, Y and Z. The Aliquots were run on an Agilent® Bioanalyzer 4150 using the RNA Screen tape. RIN values and O.D. ratios confirm the overall integrity and purity of the RNA.

Figure 2. High quality qPCR curves demonstrating accurate detection and quantitation of targets. To demonstrate compatibility with downstream applications, samples were subsequently used for RT-PCR (+/RT) for detection of Yeast RNA species using iTaq™ Universal SYBR® Green Supermix (BioRad 1725121). Purified RNA from S. cerevisiae (Figure 2A) and P. pastoris (Figure 2B) was diluted to produce a five-log range of input template concentrations. Primers targeting Actins were used for RT-qPCR assays, assembled as directed by iTaq™ Universal SYBR® Green One-Step Kit RT-qPCR reagents (BioRad 1725150) and cycled on a QuantStudio3 (Applied Biosystems, by ThermoScientific)

Figure 3. SPINeasy® RNA Kit is validated on two types of yeast species (S. cerevisiae and P. pastoris). The kit may also be used for other species of yeast. Handling of more than 2 x 10⁸ yeast cells is possible, however it may require further DNase I treatment (to be standardized according to the manufacturer’s guidelines).