SPINeasy Yeast Plasmid Miniprep Kit is a high-performance plasmid extraction kit based on silica-membrane spin-column technology. This kit has demonstrated the capability to isolate up to 20 ng of pGADT7-based plasmid from 1.5 mL of overnight cultures. The recovered plasmid DNA can be used for PCR, qPCR, and E. coli transformation. There is no need for the use of organic solvents such as phenol or chloroform.

The SPINeasy Yeast Plasmid Miniprep Kit has been rigorously validated to evaluate its performance. The following results demonstrate the extraction of plasmid with the SPINeasy Yeast Plasmid Miniprep kit. High-yield plasmid, suitable for downstream applications such as qPCR, was obtained.

Figure 1: The quantity of pGADT7 plasmids extracted from yeast using the SPINeasy Yeast Plasmid Miniprep Kit (MP) was compared with competitors Z, T, and K. The yields were measured using the Qubit 4 Fluorometer.

Figure 2: The amplifiability of pGADT7 plasmids, extracted from yeast using the SPINeasy Yeast Plasmid Miniprep Kit (MP), was compared against competitors Z, T, and K. 1 mL of plasmid was amplified using a specific set of primers, with targets being detected using SYBR Green technology.