Description
• Intended Use: Standard Methods Agar is used for the enumeration of bacteria in water, wastewater, food, and dairy products.
This formula conforms to American Public Health Association1 (APHA), and Association of Official Analytical Chemists
(AOAC).
• Product Summary and Explanation: Standard Methods Agar was developed by Buchbinder, Baris, and Goldstein in 1953 at the
request of the American Public Health Association. Results showed that a dehydrated milk-free medium containing 0.25% Yeast
Extract, 0.5% Tryptone, 0.1% Dextrose, and 1.5% Agar per liter approximated the productivity of Tryptone Glucose Extract
Agar with added milk. Buchbinder et al. recommended that a dehydrated culture medium be used in preparing the standard plate
count medium rather than preparing the medium from ingredients. Standard Methods Agar as originally suggested by Buchbinder
et al. Standard Methods Agar is also referred to as Plate Count Agar and Tryptone Glucose Yeast Agar. This formula is specified
in standard method procedures.
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