Description
GenScript Streptavidin Resin is an affinity chromatography medium designed for easy,
one-step purification of biotinylated peptides, antibodies, lectins, etc
from samples. The purified recombinant streptavidin is covalently coupling to 4%
highly cross-linked agarose. The
coupling is optimized to give high binding capacity for biotinylated molecules. The total
binding capacity of Streptavidin Resin is more
than 120 nmol of D-Biotin/ml
settled resin. Table 1 lists the characteristics of Streptavidin Resin.
Streptavidin is a biotin-binding protein found in the culture broth of the bacterium Streptomyces
avidinii. Streptavidin binds 4 moles of biotin per mole of protein with an
extremely high affinity. Streptavidin lacks carbohydrate side chains present on
avidin and has an isoelectric point of 6.5 (vs 10 for avidin) close to
where most useful biological interactions occur. As a result,
streptavidin frequently exhibits much lower non-specific binding than avidin
does.