Description
Applied Biosystems™ SYBR™ Green PCR Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.
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- SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required
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- AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
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- dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
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- Proprietary buffer enhancements ensure performance and reliability
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- Premixed components stored at 2° to 8°C significantly reduce assay setup time
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Maximum Flexibility and Convenience:
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- Applied Biosystems™ SYBR™ Green PCR Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required
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- SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR
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- The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity
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- Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations
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- This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation
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- SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.
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- Store at -20°C
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Order Info
Shipping Condition: Wet Ice