Thermo Scientific™ Blocker™ BSA (10X) in PBS

Thermo Scientific™ Blocker BSA Blocking Buffers are ready-to-use, 10X PBS or TBS solutions of bovine serum albumin protein for blocking steps in Western blotting, ELISA, immunohistochemistry and nucleic acid detection methods.

nThese blocking buffers are 10% (w/v) solutions of high-quality BSA that are useful for saturating excess protein-binding sites on membranes and microplates in immunoassays. Blocker BSA Buffers are most frequently diluted 10-fold (to 1% BSA) for initial testing, but other buffer concentrations can be beneficial for specific systems. Blocker BSA is usually more effective than nonfat milk blocking buffers for biotin-avidin systems because it contains a single purified protein that is devoid endogenous biotin.nnHighlights:n

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  • Purified protein – 10% solutions of high-quality bovine serum albumin; a single purified protein provides fewer chances of cross-reaction with assay components than serum or milk solutions

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• • Convenient – concentrated formulation saves storage space and can be diluted easily to obtain optimal blocking results for specific applications

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• • Easy to use – no waiting for powder to dissolve with this ready-to-dilute liquid concentrate

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• • Flexible – available in PBS and TBS formulations to suit a variety of applications

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