Thermo Scientific™ Fluoraldehyde™ o-Phthaldialdehyde Crystals (OPA)

$598.90

Thermo Scientific™ Pierce Fluoraldehyde (OPA) is o-phthalaldehyde, a highly sensitive fluorescent pre- or post-column derivatization reagent for peptide or amino acid detection and quantitation in HPLC.

Fluoraldehyde Crystals are a specially purified, fluorogenic-grade preparation of o-phthalaldehyde (OPA). This amine-reactive compound is primarily used for high-sensitivity, fluorescent detection of primary amines, including amino acids, peptides, proteins and polyamines in pre- or post-column chromatographic effluents.

Highlights:

  • For pre- or post-column amino acid derivatization for fluorescent detection and quantitation
  • Reacts with all primary amine-containing analytes to yield fluorescent derivatives (ex/em = 340nm/455nm)
  • Provides an accurate measure of both composition and absolute protein-peptide content
  • Ideal for work with recombinant proteins and synthetic peptides
  • Can be used for fluorescent protein or peptide assay
  • Can be prepared as a stable aqueous solution in borate (see instructions)

Reagent Properties:

  • Can be prepared as a stable aqueous solution in borate (see instructions)
  • Alternative names: Fluoraldehyde Crystals, OPA
  • Chemical name: ortho-Phthalaldehyde
  • Reactive toward: Primary amines (–NH2)
  • Chemical formula: C8H6O2
  • CAS number: 643-79-8
  • Molecular weight: 134.12
  • Form: Pale yellow, free-flowing crystals
Weight 10.00 lbs
Dimensions 10.00 × 10.00 × 10.00 in
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SKU: ASR-26015
SKU: ASR-26015 Category: Brand:

Description

Measure peptides or amino acids (Ex 340nm, Em 455nm) with phthalaldehyde, a fluorescent, amine-reactive, HPLC pre- or post-column labeling reagent.

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