Thermo Scientific™ Fluoraldehyde™ o-Phthaldialdehyde Reagent Solution (OPA)

Thermo Scientific™ Pierce Fluoraldehyde (OPA) Reagent is a ready-to-use solution of o-phthalaldehyde, a fluorescent pre- or post-column derivatization reagent for peptide or amino acid detection and quantitation in HPLC.

Fluoraldehyde Reagent contains OPA (o-phthalaldehyde), which reacts with primary amines of amino acids, peptide and proteins to enable fluorescent detection and quantitation. The reagent that can be used as a protein or peptide assay reagent or as a pre- or post-column detection reagent for amino acid analysis (HPLC). Reaction of OPA with proteins and peptides yields linear results over a wide range of concentrations. The Fluoraldehyde Reagent Solution is supplied ready to use and enables fast quantitation of proteins or peptides in solution.

Highlights:

• For pre- or post-column amino acid derivatization for fluorescent detection and quantitation
• Reacts with all primary amine-containing analytes to yield fluorescent isoindole derivatives
• Provides an accurate measure of both composition and absolute protein-peptide content
• Ideal for work with recombinant proteins and synthetic peptides
• Can be used for fluorescent protein or peptide assay
• Pre-column derivatization mixtures can be injected into LC without any processing

Reagent Properties:

• Formulation: 0.8mg/mL o-phthalaldehyde (OPA) in proprietary buffer, pH 10
• Form: Clear, colorless to slightly yellow liquid
• Reactive toward: Primary amines (–NH2)
• Detection: Fluorescence (excitation/emission = 340nm/455nm)
• Application: Pre- or post-column derivatization for detection by HPLC