Description
Trichrome Stain Kit (Connective Tissue Stain) ab150686 is intended for use in the histological visualization of collagenous connective tissue fibers in tissue sections.
The trichrome stain kit may be used on formalin-fixed, paraffin-embedded. For frozen sections, it will require optimization by the user.
Trichrome stain protocol summary:
– deparaffinize sections if necessary
– preheat Bouin’s Fluid to 54-64°C
– incubate slide in preheated Bouin’s Fluid for 60 min and cool for 10 min
– rinse in water
– incubate slide in Weigert’s Iron Hematoxylin for 5 min
– rinse in water
– incubate slide in Biebrich Scarlet / Acid Fuchsin solution for 15 min
– rinse in water
– differentiate in phosphomolybdic / phosphotungstic acid solution for 10-15 min
– incubate slide in Aniline Blue solution for 5-10 min
– rinse in water
– incubate slide in acetic acid solution for 3-5 min
– dehydrate, clear and mount