TrypLE™ Express Enzyme (1X), phenol red

Used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. TrypLE™ Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts.

• • Gentle On Cells

n

• • urity increases specificity due to the action of single enzyme, reducing damage caused by cleavage from enzymes present in some trypsin preparations and in other protease extracts

n

• • Room-Temperature Stable for 24 months

n

• • Ready to use

n

• • Can be directly substituted for trypsin in existing protocols

n

• • Dilution alone inactivates TrypLE Express, avoiding the need for trypsin inhibitors, such as FBS

n

Energy efficient, less hazardous, sustainable packagingnnCell Culture, Mammalian Cell Culture