Description
The MP Biomedicals Ultra-Sense Femto-Plus Western ECL Substrate, a luminol-based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The product is designed for the detection of target proteins in amounts that are too small to be detected with typical ECL substrates. The low femtogram detection (mid-zeptomole) of antigen is enabled by Ultra-Sense Femto-Plus Western ECL Substrate’s excellent sensitivity and long signal duration. Furthermore, its long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.