Dry Ice

The 100 bp DNA Ladder is a ready-to-use DNA Ladder, which is pre-mixed with loading dye for direct gelloading. This product consists of 11 individual DNA fragments: 100 bp, 200 bp, 300 bp, 400 bp, 500 bp, 600 bp, 700 bp, 800 bp, 900 bp, 1000 bp and 1500 bp, derived from amixture of PCR products and specifically digested plasmid DNA. The 500 bp fragment is present at increased intensity for easy identification.

The 100 bp DNA Ladder is a ready-to-use DNA Ladder, which is pre-mixed with loading dye for direct gelloading. This product consists of 11 individual DNA fragments: 100 bp, 200 bp, 300 bp, 400 bp, 500 bp, 600 bp, 700 bp, 800 bp, 900 bp, 1000 bp and 1500 bp, derived from amixture of PCR products and specifically digested plasmid DNA. The 500 bp fragment is present at increased intensity for easy identification.

10X ILM PCR Primers

10X ILM PCR Primers

10X MGI UDI Primers

10X MGI UDI Primers

2X FASA™ Genotyping Master Mix (Standard ROX)

2X FASA™ Genotyping Master Mix (Standard ROX)

2X MultiF Seamless Assembly Mix

2X MultiF Seamless Assembly Mix

Real-time Quantitative PCR (qPCR) is a powerful technique in detecting initial DNA input in a PCR reaction by fluorescence signal accumulation. The DNA double strand bonded dye, SYBR® Green I is the most commonly used dye in qPCR. ABclonal 2X Universal SYBR Green Fast qPCR Mix contains de novel designed universal reference dye, which can realize higher signal resolution and suits for all currently used qPCR instruments (including high ROX mode, low ROX mode and No ROX mode required machines). It also contains Hot-Start Taq DNA polymerase to avoid unexpected amplification results.
Besides, ABclonal 2X Universal SYBR Green Fast qPCR Mix is an optimized qPCR reaction mix, It contains all required components in qPCR except primers and template. It is convenient for experiment and suitable for multiple species. The above features make it as an ideal experiment tool for gene quantitative research.

Real-time Quantitative PCR (qPCR) is a powerful technique in detecting initial DNA input in a PCR reaction by fluorescence signal accumulation. The DNA double strand bonded dye, SYBR® Green I is the most commonly used dye in qPCR. ABclonal 2X Universal SYBR Green Fast qPCR Mix contains de novel designed universal reference dye, which can realize higher signal resolution and suits for all currently used qPCR instruments (including high ROX mode, low ROX mode and No ROX mode required machines). It also contains Hot-Start Taq DNA polymerase to avoid unexpected amplification results.
Besides, ABclonal 2X Universal SYBR Green Fast qPCR Mix is an optimized qPCR reaction mix, It contains all required components in qPCR except primers and template. It is convenient for experiment and suitable for multiple species. The above features make it as an ideal experiment tool for gene quantitative research.

The 5′ Deadenylase derived from yeast can remove adenosine residues from the 5′-end of DNA and RNA, retaining the 5′-end phosphate group. It can cleave AppppA to produce ATP and AMP.

5’App-T4 DNA Ligase is a mutant of T4 DNA Ligase without adenylation function, which can specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of DNA. This enzyme does not require ATP as a cofactor for ligation, but requires a pre-adenylated substrate. This enzyme reduces background ligation (chimera formation) during NGS library construction, because it can only use 5 ‘preadenylation adapter to the DNA fragments.

5’App-T4 DNA Ligase is a mutant of T4 DNA Ligase without adenylation function, which can specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of DNA. This enzyme does not require ATP as a cofactor for ligation, but requires a pre-adenylated substrate. This enzyme reduces background ligation (chimera formation) during NGS library construction, because it can only use 5 ‘preadenylation adapter to the DNA fragments.

6X DNA/RNA loading Buffer

Recombinase polymerase amplification (RPA) is a novel nucleic acid amplification technique (NAAT) that enables rapid amplification of targets at a constant low temperature (37~42°C); The target nucleic acid molecules can be amplified to detectable levels within 10-30 minutes. This kit helps user acheive high detection sensitivity, specificity, and short reaction time (only 15-25 minutes). The reaction components are in the form of lyophilized beads, which are easy to operate and store. This reagent can be used for reaction in metal baths, water baths, etc., and user doesn’t need to purchase other expensive devices.

ABclonal Zero TOPO-TA/Blunt Cloning Kit

ABQubit 1X dsDNA HS Assay Kit (Pre-Mixed)

ABQubit 1X dsDNA HS Assay Kit (Pre-Mixed)

ABQubit dsDNA HS Assay Kit

ABQubit dsDNA HS Assay Kit

ABQubit ssDNA Assay Kit

ABQubit ssDNA Assay Kit

ABScript Full Length Reverse Transcriptase for Single Cell

ABScript Full Length Reverse Transcriptase for Single Cell

ABScript GII Reverse Transcriptase is a recombinant Reverse Transcriptase derived from the bacterial group II intron. It exhibits strong thermostability and inhibitor tolerance during RNA Reverse transcription to synthesize cDNA. It is particularly suitable for reverse transcription of RNA samples containing long fragments, complex secondary structures and inhibitory components. ABScript GII Reverse Transcriptase enhances the 5′ end sequencing coverage of long transcripts, making it suitable for RNA-seq applications, such as direct RNA sequencing and long cDNA sequencing.

ABScript GII Reverse Transcriptase is a recombinant Reverse Transcriptase derived from the bacterial group II intron. It exhibits strong thermostability and inhibitor tolerance during RNA Reverse transcription to synthesize cDNA. It is particularly suitable for reverse transcription of RNA samples containing long fragments, complex secondary structures and inhibitory components. ABScript GII Reverse Transcriptase enhances the 5′ end sequencing coverage of long transcripts, making it suitable for RNA-seq applications, such as direct RNA sequencing and long cDNA sequencing.

ABScript HII Reverse Transcriptase is a genetically engineered RNA-dependent DNA polymerase with RNase H activity. The enzyme can use RNA (cDNA synthesis) or ssDNA as a template to synthesize a complementary DNA strand that can be applied to the cDNA synthesis of the first and second strands. This product is compatible with BstL DNA polymerase, especially for RT-LAMP (reverse transcription-loop-mediated isothermal amplification).

ABScript HII Reverse Transcriptase is a genetically engineered RNA-dependent DNA polymerase with RNase H activity. The enzyme can use RNA (cDNA synthesis) or ssDNA as a template to synthesize a complementary DNA strand that can be applied to the cDNA synthesis of the first and second strands. This product is compatible with BstL DNA polymerase, especially for RT-LAMP (reverse transcription-loop-mediated isothermal amplification).

ABscript II Reverse transcriptase is a recombinant M-MuLV reverse transcriptase with low RNase H activity and enhanced thermal stability.Compared to wild-type M-MuLV,recombinant M-MuLV reverse transcriptase can synthesize first-strand cDNA at higher temperatures.The enzyme is active at up to 48℃ with higher specificity and cDNA yield.The kit provides two reverse transcription primers.The Oligo-dT primer [d(T)₂₃VN] forces the primer to anneal to the beginning of the polyA tail.The optimized random primer premix has low specificity,and all RNA,including mRNA,rRNA,and tRNA can be used as a reverse transcription template.This kit can amplify cDNA up to 10 kb.

ABScript II One Step SYBR Green RT-qPCR Kit is a special kit for one-step RT-qPCR reaction by using SYBR Green I chimeric fluorescence. The kit takes RNA as the template, uses gene-specific primers, and reverse transcription and PCR reaction can be carried out continuously in the same tube without additional pipe opening and pipetting operations, greatly improving the detection flux and effectively preventing contamination. This reaction system can detect the amplification products in real time, greatly improving the detection sensitivity, and omitting the electrophoresis step after PCR reaction, which is very suitable for the detection of RNA virus and other trace RNA. This product integrates the superiority of ABScript II Reverse Transcriptase and Taq DNA Polymerase, cooperate with optimized buffer system, with high amplification efficiency and high amplification specificity, one-step RT – qPCR reaction can be stable. In addition, all the enzymes used in the reaction are made into enzyme Mix, which is easier and more convenient to operate.

ABScript II One Step SYBR Green RT-qPCR Kit is a special kit for one-step RT-qPCR reaction by using SYBR Green I chimeric fluorescence. The kit takes RNA as the template, uses gene-specific primers, and reverse transcription and PCR reaction can be carried out continuously in the same tube without additional pipe opening and pipetting operations, greatly improving the detection flux and effectively preventing contamination. This reaction system can detect the amplification products in real time, greatly improving the detection sensitivity, and omitting the electrophoresis step after PCR reaction, which is very suitable for the detection of RNA virus and other trace RNA. This product integrates the superiority of ABScript II Reverse Transcriptase and Taq DNA Polymerase, cooperate with optimized buffer system, with high amplification efficiency and high amplification specificity, one-step RT – qPCR reaction can be stable. In addition, all the enzymes used in the reaction are made into enzyme Mix, which is easier and more convenient to operate.

ABScript II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 48°C, providing higher specificity, higher yield of cDNA and more full-length cDNA product up to 12 kb.

ABScript II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 48°C, providing higher specificity, higher yield of cDNA and more full-length cDNA product up to 12 kb.

ABScript III One Step RT-qPCR Probe Kit with UDG V5 is a universal kit for one step RT-qPCR reactions using the probe method. This kit uses RNA as the template, reverse transcription and PCR reactions can be performed continuously in the same tube, which improves the detection throughput. This kit introduces the dUTP/UDG anti-contamination system, heat-sensitive UDG can rapidly degrade U-containing contaminants at room temperature; and the heat-sensitive UDG is rapidly inactivated during reverse transcription at 50℃, which will not affect the efficiency and sensitivity of RT-qPCR. The reaction system can perform real-time detection of amplified products and improve the detection sensitivity, which is very suitable for the detection of trace RNA such as RNA viruses.

ABScript III One Step RT-qPCR Probe Kit with UDG V5 is a universal kit for one step RT-qPCR reactions using the probe method. This kit uses RNA as the template, reverse transcription and PCR reactions can be performed continuously in the same tube, which improves the detection throughput. This kit introduces the dUTP/UDG anti-contamination system, heat-sensitive UDG can rapidly degrade U-containing contaminants at room temperature; and the heat-sensitive UDG is rapidly inactivated during reverse transcription at 50℃, which will not affect the efficiency and sensitivity of RT-qPCR. The reaction system can perform real-time detection of amplified products and improve the detection sensitivity, which is very suitable for the detection of trace RNA such as RNA viruses.

ABScript III Reverse Transcriptase is a genetically engineered M-MuLV reverse transcriptase with reduced RNase H activity and improved thermal stability. Compared with wild-type M-MuLV, it can synthesize first-strand cDNA at higher temperature. Product still has features such as high activity at 50-55℃, higher specificity, higher yield, and the ability to synthesize cDNA up to 12 kb.

ABScript III Reverse Transcriptase is a genetically engineered M-MuLV reverse transcriptase with reduced RNase H activity and improved thermal stability. Compared with wild-type M-MuLV, it can synthesize first-strand cDNA at higher temperature. Product still has features such as high activity at 50-55℃, higher specificity, higher yield, and the ability to synthesize cDNA up to 12 kb.

ABScript III RT Master Mix for qPCR is developed based on ABScript II Reverse Transcriptase and suitable for two-step RT-qPCR detection. 5X ABScript III RT Mix contains all the reagents needed for reverse transcription reaction (ABScript III Reverse Transcriptase, RNase Inhibitor, RandomPrimer, Oligo dT Primer, dNTP and reaction buffer), and a reaction can be started simply by adding template RNA and Nuclease-free H₂O.
This product is specially optimized for qPCR. The proportionally optimized Random Primers/Oligo (dT)₂₀VN Primer Mix enables cDNA synthesis to progress from each region of RNA transcription efficiently, which ensures the authenticity and repeatability of qPCR results to the greatest extent. Reverse transcription products are compatible with SYBR Green and probe qPCR and can be used in combination with corresponding reagents according to experimental purposes for high-performance gene expression analysis.

ABScript III RT Master Mix for qPCR is developed based on ABScript II Reverse Transcriptase and suitable for two-step RT-qPCR detection. 5X ABScript III RT Mix contains all the reagents needed for reverse transcription reaction (ABScript III Reverse Transcriptase, RNase Inhibitor, RandomPrimer, Oligo dT Primer, dNTP and reaction buffer), and a reaction can be started simply by adding template RNA and Nuclease-free H₂O.
This product is specially optimized for qPCR. The proportionally optimized Random Primers/Oligo (dT)₂₀VN Primer Mix enables cDNA synthesis to progress from each region of RNA transcription efficiently, which ensures the authenticity and repeatability of qPCR results to the greatest extent. Reverse transcription products are compatible with SYBR Green and probe qPCR and can be used in combination with corresponding reagents according to experimental purposes for high-performance gene expression analysis.

ABScript III RT Master Mix for qPCR with gDNA Remover is developed based on ABScript III Reverse Transcriptase and suitable for two-step RT-qPCR detection. The 5X ABScript III RT Mix in this product contains all the reagents required for the reverse transcription reaction. The reaction protocol is simple and can be carried out quickly by adding the RNA template and H2O. The gDNA Remover Mix in this product can completely remove the genomic DNA remaining in the RNA template and make the qPCR results more accurate. The dsDNase is heat-sensitive and can be quickly and irreversibly inactivated under high temperature conditions. Therefore, it only needs one sample to be used to remove genomic DNA contamination and reverse transcription reactions in the same tube.
This product is specially optimized for qPCR. The proportionally optimized Random Primers/Oligo (dT)₂₀VN Primer Mix enables cDNA synthesis to progress from each region of RNA transcription efficiently, which ensures the authenticity and repeatability of qPCR results to the greatest extent. Reverse transcription products are compatible with SYBR Green and probe qPCR and can be used in combination with corresponding reagents according to experimental purposes for high-performance geneexpression analysis.

ABScript III RT Master Mix for qPCR with gDNA Remover is developed based on ABScript III Reverse Transcriptase and suitable for two-step RT-qPCR detection. The 5X ABScript III RT Mix in this product contains all the reagents required for the reverse transcription reaction. The reaction protocol is simple and can be carried out quickly by adding the RNA template and H2O. The gDNA Remover Mix in this product can completely remove the genomic DNA remaining in the RNA template and make the qPCR results more accurate. The dsDNase is heat-sensitive and can be quickly and irreversibly inactivated under high temperature conditions. Therefore, it only needs one sample to be used to remove genomic DNA contamination and reverse transcription reactions in the same tube.
This product is specially optimized for qPCR. The proportionally optimized Random Primers/Oligo (dT)₂₀VN Primer Mix enables cDNA synthesis to progress from each region of RNA transcription efficiently, which ensures the authenticity and repeatability of qPCR results to the greatest extent. Reverse transcription products are compatible with SYBR Green and probe qPCR and can be used in combination with corresponding reagents according to experimental purposes for high-performance geneexpression analysis.

ABScript III WS Reverse Transcriptase is a genetically engineered M-MuLV reverse transcriptase with reduced RNase H activity. It is a body-blocked warm-start enzyme that inhibits reverse transcriptase activity below 40°C. The enzyme synthesizes a complementary cDNA strand using RNA as a template. This product is highly compatible with Bst DNA Polymerase and is specifically suitable for RT-LAMP applications.

ABScript III WS Reverse Transcriptase is a genetically engineered M-MuLV reverse transcriptase with reduced RNase H activity. It is a body-blocked warm-start enzyme that inhibits reverse transcriptase activity below 40°C. The enzyme synthesizes a complementary cDNA strand using RNA as a template. This product is highly compatible with Bst DNA Polymerase and is specifically suitable for RT-LAMP applications.

This kit is suitable for cDNA first strand synthesis using microRNA as template through the tail addition method, where the Poly (A) tail addition reaction and reverse transcription reaction at the 3 ‘end of miRNA can be efficiently carried out simultaneously. ABScript miRNA-A Enzyme Mix contains Poly (A) Polymerase (PAP) and reverse transcriptase. PAP is mainly used to add Poly (A) tails at the 3 ‘end of RNA molecules, and can also specifically recognize single stranded RNA, effectively avoiding RT reactions of pre-miRNA with double stranded or stem-loop structures. The modified reverse transcriptase lacks of RNase H activity and increases its affinity with RNA, resulting in a significant improvement in the efficiency and sensitivity of miRNA reverse transcription. The obtained cDNA can be directly used for qPCR detection using either SYBR Green dye-base or Taqman probe-base reagent.

ABScript Neo RT Master Mix for qPCR is an efficient and fast cDNA first-strand synthesis master mix, suitable for two-step RT-qPCR detection. The 4X ABScript Neo RT Mix in this product contains all the reagents required for the reverse transcription reaction. The reaction protocol is simple and can be carried out quickly by adding the RNA template and H₂O.
This product is specially optimized for qPCR. The proportionally optimized Random Primers/Oligo (dT)20VN Primer Mix enables cDNA synthesis to progress from each region of RNA transcription efficiently, which ensures the authenticity and repeatability of qPCR results to the greatest extent. Reverse transcription products are compatible with SYBR Green and probe qPCR and can be used in combination with corresponding reagents according to experimental purposes for high-performance gene expression analysis.

ABScript Neo RT Master Mix for qPCR with gDNA Remover is an efficient and fast cDNA first-strand synthesis master mix, suitable for two-step RT-qPCR detection. The 4X ABScript Neo RT Mix in this product contains all the reagents required for the reverse transcription reaction. The reaction protocol is simple and can be carried out quickly by adding the RNA template and H₂O. The gDNA Remover Mix in this product can completely remove the genomic DNA remaining in the RNA template and make the qPCR results more accurate. The dsDNase is heat-sensitive and can be quickly and irreversibly inactivated under high temperature conditions. Therefore, it only needs one sample to be used to remove genomic DNA contamination and reverse transcription reactions in the same tube.