Dry Ice

Gloria Nova HS DNA polymerase is a typical B-family polymerase with a unique structure. It is a new enzyme similar to Pyrococcus furiosus, fused with a continuous synthesis enhancement domain, which improves elongation, yield and specificity.
Gloria Nova HS DNA polymerase is equipped with specific antibodies, which effectively inhibits non-specific amplification and improves the stability of Gloria Nova HS DNA polymerase.
Gloria Nova HS DNA polymerase has 5′-3′ continuous synthesis activity and 3′-5′ exonuclease activity, and its amplified products are blunt-ended.
Gloria Nova DNA polymerase is a mutant polymerase modified by genetic engineering. It has the ability to incorporate dUTP and can also amplify complex templates with dU.
Gloria Nova HS 2X PCR Mix for NGS is an easy-to-operate 2X Mix, which contains Gloria Nova HS DNA polymerase, buffer, Mg⁺ and dNTPs, etc. Only additional DNA template, primers and water are needed in the amplification system. Perform amplification.
All batches of Gloria Nova HS 2X PCR Mix for NGS have undergone strict quality control to ensure the stable performance of each batch of reagents.

Gloria Nova HS DNA polymerase is a typical B-family polymerase with a unique structure. It is a new enzyme similar to Pyrococcus furiosus, fused with a continuous synthesis enhancement domain, which improves elongation, yield and specificity.
Gloria Nova HS DNA polymerase is equipped with specific antibodies, which effectively inhibits non-specific amplification and improves the stability of Gloria Nova HS DNA polymerase.
Gloria Nova HS DNA polymerase has 5′-3′ continuous synthesis activity and 3′-5′ exonuclease activity, and its amplified products are blunt-ended.
Gloria Nova DNA polymerase is a mutant polymerase modified by genetic engineering. It has the ability to incorporate dUTP and can also amplify complex templates with dU.
Gloria Nova HS 2X PCR Mix for NGS is an easy-to-operate 2X Mix, which contains Gloria Nova HS DNA polymerase, buffer, Mg⁺ and dNTPs, etc. Only additional DNA template, primers and water are needed in the amplification system. Perform amplification.
All batches of Gloria Nova HS 2X PCR Mix for NGS have undergone strict quality control to ensure the stable performance of each batch of reagents.

Gloria Nova HS DNA polymerase is an ideal enzyme for high fidelity PCR with excellent processivity. It is a novel engineered enzyme with comparable performance to Pyrococcus furiosus DNA polymerase.
With unique structure Gloria Nova HS DNA polymerase contains a recombinant synthesis enhancement domain to increase fidelity and extension speed. The antibody-mediated hot-start feature significantly inhibits non-specific amplifications at room temperature.
Gloria Nova is one of the thermostable DNA polymerases with strong 3’-5’ exonuclease activity (proofreading activity), which results in its extreme high fidelity, 10-15 times higher than Taq DNA polymerase and 6 times higher than Pyrococcus furiosus DNA polymerase.
The Gloria Nova HS PCR Kit is supplied with a 5X Gloria Nova HF Buffer and a 2.5X Gloria Nova GC Buffer. The 5X Gloria Nova HF Buffer is an optimized buffer for general high fidelity amplifications while the 2.5X Gloria Nova GC Buffer is used in the amplifications of problematic or GC-rich templates.

Gloria Nova HS DNA polymerase is an ideal enzyme for high fidelity PCR with excellent processivity. It is a novel engineered enzyme with comparable performance to Pyrococcus furiosus DNA polymerase.
With unique structure Gloria Nova HS DNA polymerase contains a recombinant synthesis enhancement domain to increase fidelity and extension speed. The antibody-mediated hot-start feature significantly inhibits non-specific amplifications at room temperature.
Gloria Nova is one of the thermostable DNA polymerases with strong 3’-5’ exonuclease activity (proofreading activity), which results in its extreme high fidelity, 10-15 times higher than Taq DNA polymerase and 6 times higher than Pyrococcus furiosus DNA polymerase.
The Gloria Nova HS PCR Kit is supplied with a 5X Gloria Nova HF Buffer and a 2.5X Gloria Nova GC Buffer. The 5X Gloria Nova HF Buffer is an optimized buffer for general high fidelity amplifications while the 2.5X Gloria Nova GC Buffer is used in the amplifications of problematic or GC-rich templates.

Gloria U Hot-Start DNA Polymerase is a DNA polymerase obtained through molecular modification, which can effectively amplify templates containing uracil. The uracil-binding ability of Gloria U polymerase is inactivated, allowing it to efficiently amplify DNA that has been treated with bisulfite. Moreover, this enzyme exhibits the same high yield and low GC bias as Gloria Nova Hot-Start DNA Polymerase.
Gloria U Hot-Start High-Fidelity DNA Polymerase possesses 5′-3′ polymerase and 3′→5′ exonuclease (proofreading) activities, but lacks 5′-3′ exonuclease activity.
The Gloria U 2X HS Master Mix contains Gloria U DNA Polymerase, dNTPs, 5X Gloria U Reaction Buffer, and other components. Only template and primers need to be added.

Gloria U Hot-Start DNA Polymerase is a DNA polymerase obtained through molecular modification, which can effectively amplify templates containing uracil. The uracil-binding ability of Gloria U polymerase is inactivated, allowing it to efficiently amplify DNA that has been treated with bisulfite. Moreover, this enzyme exhibits the same high yield and low GC bias as Gloria Nova Hot-Start DNA Polymerase.
Gloria U Hot-Start High-Fidelity DNA Polymerase possesses 5′-3′ polymerase and 3′→5′ exonuclease (proofreading) activities, but lacks 5′-3′ exonuclease activity.
The Gloria U 2X HS Master Mix contains Gloria U DNA Polymerase, dNTPs, 5X Gloria U Reaction Buffer, and other components. Only template and primers need to be added.

Gloria U Hot-Start DNA Polymerase is a typical Family B polymerase with unique characteristics. Through molecular modification of the Gloria polymerase, the binding capability of uracil has been inactivated. As a result, Gloria U can efficiently amplify bisulfite-treated DNA or libraries containing uracil. The enzyme exhibits the same high yield, low GC bias, and uniform coverage as the Gloria Nova Hot-Start DNA Polymerase. The Gloria U 2X Mix for NGS has 5′-3′ polymerase activity and 3′→5′ exonuclease (proofreading) activity, making it suitable for amplifying templates containing uracil or amplifying libraries containing uracil.

Gloria U Hot-Start DNA Polymerase is a typical Family B polymerase with unique characteristics. Through molecular modification of the Gloria polymerase, the binding capability of uracil has been inactivated. As a result, Gloria U can efficiently amplify bisulfite-treated DNA or libraries containing uracil. The enzyme exhibits the same high yield, low GC bias, and uniform coverage as the Gloria Nova Hot-Start DNA Polymerase. The Gloria U 2X Mix for NGS has 5′-3′ polymerase activity and 3′→5′ exonuclease (proofreading) activity, making it suitable for amplifying templates containing uracil or amplifying libraries containing uracil.

Gloria U Hot-Start DNA Polymerase is a DNA polymerase obtained through Gloria modification, which can effectively amplify templates containing uracil. The uracil-binding ability of Gloria U polymerase is inactivated, allowing it to efficiently amplify bisulfite-treated DNA. Moreover, this enzyme exhibits the same high yield as Gloria Nova Hot-Start DNA Polymerase. Gloria U Hot-Start High-Fidelity DNA Polymerase possesses 5′-3′ polymerase and 3′→5′ exonuclease (proofreading) activities but lacks 5′-3′ exonuclease activity. This polymerase can effectively incorporate dUTP and efficiently amplify template DNA containing dUTP. It also has the ability to recognize primers containing dUTP and complete the amplification process.

Gloria U Hot-Start DNA Polymerase is a DNA polymerase obtained through Gloria modification, which can effectively amplify templates containing uracil. The uracil-binding ability of Gloria U polymerase is inactivated, allowing it to efficiently amplify bisulfite-treated DNA. Moreover, this enzyme exhibits the same high yield as Gloria Nova Hot-Start DNA Polymerase. Gloria U Hot-Start High-Fidelity DNA Polymerase possesses 5′-3′ polymerase and 3′→5′ exonuclease (proofreading) activities but lacks 5′-3′ exonuclease activity. This polymerase can effectively incorporate dUTP and efficiently amplify template DNA containing dUTP. It also has the ability to recognize primers containing dUTP and complete the amplification process.

Golden Gate 2X Assembly Mix (BsmBI)

Golden Gate 2X Assembly Mix (BsmBI)

Golden Gate 2X Assembly Mix (Eco31Ⅰ)

Golden Gate 2X Assembly Mix (Eco31Ⅰ)

5’… G G /C C…3′
3’… C C /G G…5′
Isoschizomers: BsuRI, BshFI, BsnI, BspANI

Heat-labile UDG was derived from psychrophilic marine bacteria. It can catalyze the release of free uracil from uracil (U)-containing DNA and is inactive against RNA. This product is sensitive to high temperature, and it can cause irreversible inactivation of the enzyme above 50℃, so it is suitable for PCR、qPCR、RT-PCR、RT-qPCR system.

Heat-labile UDG was derived from psychrophilic marine bacteria. It can catalyze the release of free uracil from uracil (U)-containing DNA and is inactive against RNA. This product is sensitive to high temperature, and it can cause irreversible inactivation of the enzyme above 50℃, so it is suitable for PCR、qPCR、RT-PCR、RT-qPCR system.

Heat-T4 DNA Ligase is a thermostable mutant of T4 DNA Ligase that catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini in duplex DNA or RNA. It is designed to function at higher temperatures than wild-type T4 DNA ligase. The catalytic reaction requires ATP as a cofactor. Heat-T4 DNA Ligase will join blunt end and cohesive end termini at temperatures as high as 45ºC , and can also repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. At the same time, T4 DNA ligase closes the gaps in these DNA substrates.

Heat-T4 DNA Ligase is a thermostable mutant of T4 DNA Ligase that catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini in duplex DNA or RNA. It is designed to function at higher temperatures than wild-type T4 DNA ligase. The catalytic reaction requires ATP as a cofactor. Heat-T4 DNA Ligase will join blunt end and cohesive end termini at temperatures as high as 45ºC , and can also repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. At the same time, T4 DNA ligase closes the gaps in these DNA substrates.

High Salt-T4 DNA Ligase is a salt-tolerant mutant of T4 DNA Ligase that catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini in duplex DNA or RNA. It is designed to function at higher salt concentrations than wild type T4 DNA Ligase. The catalytic reaction requires ATP as a cofactor. High Salt-T4 DNA Ligase will join blunt end and cohesive end termini at salt concentrations as high as 300 mM without any loss in activity, and can also repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. At the same time, T4 DNA ligase closes the gaps in these DNA substrates.

High Salt-T4 DNA Ligase is a salt-tolerant mutant of T4 DNA Ligase that catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini in duplex DNA or RNA. It is designed to function at higher salt concentrations than wild type T4 DNA Ligase. The catalytic reaction requires ATP as a cofactor. High Salt-T4 DNA Ligase will join blunt end and cohesive end termini at salt concentrations as high as 300 mM without any loss in activity, and can also repair single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. At the same time, T4 DNA ligase closes the gaps in these DNA substrates.

5’…A/ A G C T T…3′
3’…T T C G A/ A…5′

5’…A/ A G C T T…3′
3’…T T C G A/ A…5′

5’…G T T/ A A C…3′
3’…C A A/ T T G…5′
Isoschizomers: KspAI

5’…C/CGG…3′
3’…GGC/C…5′
Isoschizomers: MspI、HapII、BsiSI

HS Taq DNA Polymerase (5,000 U/mL)

HS Taq DNA Polymerase (5,000 U/mL)