Dry Ice

5’…GGTAC/C…3′
3’…C/CATGG…5′

Lambda Exonuclease digests linear or nicked dsDNA in the 5′ to 3′ ́direction. It exhibits the highest cleavage activity towards 5′-phosphorylated dsDNA, showing low enzymatic activity toward ssDNA and non-phosphorylated DNA. It remains inactive at DNA nicks but shows limited activity at gaps.

Lambda Exonuclease digests linear or nicked dsDNA in the 5′ to 3′ ́direction. It exhibits the highest cleavage activity towards 5′-phosphorylated dsDNA, showing low enzymatic activity toward ssDNA and non-phosphorylated DNA. It remains inactive at DNA nicks but shows limited activity at gaps.

LB Agar Plate (Ampicillin)

Lb Cas12a Nuclease from Lachnospiraceae bacterium is a site-specific DNA endonuclease guided by a single 40-44 nucleotide guide RNA (crRNA). Targeting requires a crRNA complementary to the target site as well as a 5’ TTTV protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. Cleavage by LbCas12a Nuclease occurs ~17 bases 3′ of the PAM and leaves overhanging ends.This product also has a trans-cleavage activity, which produces high non-specific ssDNA cleavage activity when the target dsDNA, crRNA, and Cas12a protein form a ternary complex.

Lb Cas12a Nuclease from Lachnospiraceae bacterium is a site-specific DNA endonuclease guided by a single 40-44 nucleotide guide RNA (crRNA). Targeting requires a crRNA complementary to the target site as well as a 5’ TTTV protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. Cleavage by LbCas12a Nuclease occurs ~17 bases 3′ of the PAM and leaves overhanging ends.This product also has a trans-cleavage activity, which produces high non-specific ssDNA cleavage activity when the target dsDNA, crRNA, and Cas12a protein form a ternary complex.

The Lyophilization One-Step RT-qPCR Probe Kit is a universal lyophilized reagent designed for RT-qPCR reactions using probes. It is suitable for lyophilization of freeze-dried powder and freeze-dried microspheres. After freeze-drying into microspheres. This kit includes reaction buffer, hot-start Taq polymerase, novel reverse transcriptase ABScript Ⅲ Reverse Transcriptase, heat-labile UDG, and RNase inhibitor (RI). This kit utilizes RNA as a template and employs gene-specific primers. Reverse transcription and PCR reactions can be performed consecutively in the same tube, eliminating the need for additional tube opening or pipetting steps, significantly enhancing detection throughput. The kit utilizes dUTP/UDG for contamination prevention, with the heat-labile UDG quickly degrading U-containing contaminants at room temperature. The heat-labile UDG is rapidly deactivated at 50°C during reverse transcription, ensuring it does not affect the efficiency and sensitivity of RT-qPCR. This product contains glycerol content below 0.2% and includes lyoprotectants and excipients. It can be stored at 45°C for 6 months without degradation in performance.

The Lyophilization One-Step RT-qPCR Probe Kit is a universal lyophilized reagent designed for RT-qPCR reactions using probes. It is suitable for lyophilization of freeze-dried powder and freeze-dried microspheres. After freeze-drying into microspheres. This kit includes reaction buffer, hot-start Taq polymerase, novel reverse transcriptase ABScript Ⅲ Reverse Transcriptase, heat-labile UDG, and RNase inhibitor (RI). This kit utilizes RNA as a template and employs gene-specific primers. Reverse transcription and PCR reactions can be performed consecutively in the same tube, eliminating the need for additional tube opening or pipetting steps, significantly enhancing detection throughput. The kit utilizes dUTP/UDG for contamination prevention, with the heat-labile UDG quickly degrading U-containing contaminants at room temperature. The heat-labile UDG is rapidly deactivated at 50°C during reverse transcription, ensuring it does not affect the efficiency and sensitivity of RT-qPCR. This product contains glycerol content below 0.2% and includes lyoprotectants and excipients. It can be stored at 45°C for 6 months without degradation in performance.

MethyLight 2X qPCR Probe Master Mix is a probe-based reagent for real time PCR reaction. It is a powerful tool for MSP analysis of methylated DNA and multi-target detection in epigenetic research.It is an optimized premix containing DNA polymerase, dNTPs, MgCl2, KCI and other stabilizers. The optimized master mixes ensure that the PCR products in a reaction with bisulfite converted DNA as starting material are amplified with high efficiency and sensitivity. This product is a 2× concentration master mix, which contains all the components required for MethyLight qPCR reactions except primers, probes and templates.

MethyLight 2X qPCR Probe Master Mix is a probe-based reagent for real time PCR reaction. It is a powerful tool for MSP analysis of methylated DNA and multi-target detection in epigenetic research.It is an optimized premix containing DNA polymerase, dNTPs, MgCl2, KCI and other stabilizers. The optimized master mixes ensure that the PCR products in a reaction with bisulfite converted DNA as starting material are amplified with high efficiency and sensitivity. This product is a 2× concentration master mix, which contains all the components required for MethyLight qPCR reactions except primers, probes and templates.

MGI Universal Blockers-D

MGI Universal Blockers-D

MGI Universal Blockers-S

MGI Universal Blockers-S

5’…A/CGCGT…3′
3’…TGCGC/A…5′

5’…A/CGCGT…3′
3’…TGCGC/A…5′

Mouse Tissue Direct PCR Kit is specially designed for the rapid genotyping of mouse, which contains Tissue Lysis Buffer II and Powerpol HS 2X PCR Mix for Mouse Genotyping for DNA release and PCR amplification. This kit can be used for the rapid extraction of genomic DNA from mouse tails, ears, toes, and other tissues. The extracted genomic DNA can be used directly as template for PCR amplification, which greatly shortens the experimental time. Tissue Lysis Buffer II in this kit already contains proteinase K, which does not require additional preparation.
Powerpol HS 2X PCR Mix for Mouse Genotyping in this kit only needs to add primers and templates to perform amplification, and the reagent contains loading buffer, PCR products can be directly loaded for electrophoresis.

Mouse Tissue Direct PCR Kit is specially designed for the rapid genotyping of mouse, which contains Tissue Lysis Buffer II and Powerpol HS 2X PCR Mix for Mouse Genotyping for DNA release and PCR amplification. This kit can be used for the rapid extraction of genomic DNA from mouse tails, ears, toes, and other tissues. The extracted genomic DNA can be used directly as template for PCR amplification, which greatly shortens the experimental time. Tissue Lysis Buffer II in this kit already contains proteinase K, which does not require additional preparation.
Powerpol HS 2X PCR Mix for Mouse Genotyping in this kit only needs to add primers and templates to perform amplification, and the reagent contains loading buffer, PCR products can be directly loaded for electrophoresis.

The mRNA-seq Lib Prep Module for Illumina® kits provides a method for generating non-directional (non-strand-specific) RNA library that are compatible with the Illumina sequencing platform. The kit contains all of the enzymes and buffers for the First Strand Synthesis Module, Second Synthesis Module, and DNA Lib Prep Module. Its Compatible with Poly(A) mRNA Purification Module（RK20341）, rRNA Depletion module (H/M/R)（RK20348）and multiplexing adaptors and primers. The kit is applicable to animal, plant, and eukaryote (e.g., fungi) total RNA templates as well as the isolated and purified mRNA templates. The recommended total RNA input is 100 ng-1 μg.

The mRNA-seq Lib Prep Module for Illumina® kits provides a method for generating non-directional (non-strand-specific) RNA library that are compatible with the Illumina sequencing platform. The kit contains all of the enzymes and buffers for the First Strand Synthesis Module, Second Synthesis Module, and DNA Lib Prep Module. Its Compatible with Poly(A) mRNA Purification Module（RK20341）, rRNA Depletion module (H/M/R)（RK20348）and multiplexing adaptors and primers. The kit is applicable to animal, plant, and eukaryote (e.g., fungi) total RNA templates as well as the isolated and purified mRNA templates. The recommended total RNA input is 100 ng-1 μg.

5’…C/CGG…3′
3’…GGC/C…5′
Isoschizomers: HpaII、HapII、BsiSI

Mth RNA Ligase is an ATP-dependent ligase that can efficiently convert 5′-phosphorylated single-stranded DNA into 5′-adenylated DNA. The kit has been optimized to yield high quantities of adenylylated DNA regardless of whether the 3′ end of the single-stranded DNA has been aminated or otherwise modified. Typically, the modification efficiency can reach over 95%.

Mth RNA Ligase is an ATP-dependent ligase that can efficiently convert 5′-phosphorylated single-stranded DNA into 5′-adenylated DNA. The kit has been optimized to yield high quantities of adenylylated DNA regardless of whether the 3′ end of the single-stranded DNA has been aminated or otherwise modified. Typically, the modification efficiency can reach over 95%.