Dry Ice

The Rapid Plus DNA Lib Prep kit provides a streamlined and efficient method to construction DNA library for next generation sequencing (NGS) at Illumina® platforms. The entire protocol can be completed in 2 hours. Starting from fragmented input-DNA (1 – 1000 ng), the kit contains all the enzymes and buffers for end-preparation, adapter ligation and library amplification. To simplify the workflow, the fragmented DNA is end-repaired and adenylated during the initial step. The kit can be conveniently used for construction of PCR-free library from 100 ng of high-quality fragmented genomic DNA, or 10 ng of circulating cell-free DNA (cfDNA). PCR primers provided in the kit are optional for users who prefer self-selected high-fidelity DNA polymerases in the library amplification.
The kit also provides DNA repair enzymes, which could repair DNA damages such as nicks, gaps, oxidized bases, damaged/blocked 3’ ends, AP sites (apurinic/apyrimidinic sites), and uracil bases. In addition, the DNA repair enzymes significantly increase the NGS library yields from FFPE-derived DNA.

The Rapid Plus DNA Lib Prep kit provides a streamlined and efficient method to construction DNA library for next generation sequencing (NGS) at Illumina® platforms. The entire protocol can be completed in 2 hours. Starting from fragmented input-DNA (1 – 1000 ng), the kit contains all the enzymes and buffers for end-preparation, adapter ligation and library amplification. To simplify the workflow, the fragmented DNA is end-repaired and adenylated during the initial step. The kit can be conveniently used for construction of PCR-free library from 100 ng of high-quality fragmented genomic DNA, or 10 ng of circulating cell-free DNA (cfDNA). PCR primers provided in the kit are optional for users who prefer self-selected high-fidelity DNA polymerases in the library amplification.
The kit also provides DNA repair enzymes, which could repair DNA damages such as nicks, gaps, oxidized bases, damaged/blocked 3’ ends, AP sites (apurinic/apyrimidinic sites), and uracil bases. In addition, the DNA repair enzymes significantly increase the NGS library yields from FFPE-derived DNA.

The Rapid Plus DNA Lib Prep kit for Illumina® V2 provides a streamlined and efficient method to construct DNA libraries for next generation sequencing (NGS) on Illumina® sequencing platforms. This kit is an updated version of the original Rapid Plus DNA Lib Prep kit (Cat. Nos. RK20208) that has been further optimized to provide superior DNA damage repair and improve ligation efficiency with low-input DNA. The entire protocol can be completed within 2 hours. Starting from fragmented input-DNA (1 – 1000 ng), the kit contains all of the necessary enzymes and buffers for end-preparation, adapter ligation and library amplification. To simplify the workflow, fragmented DNA is end-repaired and adenylated during one initial step. The kit can be conveniently used for construction of PCR-free libraries beginning with 100 ng of high-quality fragmented genomic DNA, or from 10 ng of circulating cell-free DNA (cfDNA). PCR primers provided in the kit are optional for users who prefer self-selected high-fidelity DNA polymerases in library amplification.The kit also provides DNA repair enzymes which repair DNA damage such as nicks, gaps, oxidized bases, damaged/blocked 3’ ends, AP sites (apurinic/apyrimidinic sites), and uracil bases. In addition, the DNA repair enzymes significantly increase NGS library yields from FFPE-derived DNA.

The Rapid Plus DNA Lib Prep kit for Illumina® V2 provides a streamlined and efficient method to construct DNA libraries for next generation sequencing (NGS) on Illumina® sequencing platforms. This kit is an updated version of the original Rapid Plus DNA Lib Prep kit (Cat. Nos. RK20208) that has been further optimized to provide superior DNA damage repair and improve ligation efficiency with low-input DNA. The entire protocol can be completed within 2 hours. Starting from fragmented input-DNA (1 – 1000 ng), the kit contains all of the necessary enzymes and buffers for end-preparation, adapter ligation and library amplification. To simplify the workflow, fragmented DNA is end-repaired and adenylated during one initial step. The kit can be conveniently used for construction of PCR-free libraries beginning with 100 ng of high-quality fragmented genomic DNA, or from 10 ng of circulating cell-free DNA (cfDNA). PCR primers provided in the kit are optional for users who prefer self-selected high-fidelity DNA polymerases in library amplification.The kit also provides DNA repair enzymes which repair DNA damage such as nicks, gaps, oxidized bases, damaged/blocked 3’ ends, AP sites (apurinic/apyrimidinic sites), and uracil bases. In addition, the DNA repair enzymes significantly increase NGS library yields from FFPE-derived DNA.

The Rapid Plus DNA Lib Prep kit for Illumina® V2 provides a streamlined and efficient method to construct DNA libraries for next generation sequencing (NGS) on Illumina® sequencing platforms. This kit is an updated version of the original Rapid Plus DNA Lib Prep kit (Cat. Nos. RK20208) that has been further optimized to provide superior DNA damage repair and improve ligation efficiency with low-input DNA. The entire protocol can be completed within 2 hours. Starting from fragmented input-DNA (1 – 1000 ng), the kit contains all of the necessary enzymes and buffers for end-preparation, adapter ligation and library amplification. To simplify the workflow, fragmented DNA is end-repaired and adenylated during one initial step. The kit can be conveniently used for construction of PCR-free libraries beginning with 100 ng of high-quality fragmented genomic DNA, or from 10 ng of circulating cell-free DNA (cfDNA). PCR primers provided in the kit are optional for users who prefer self-selected high-fidelity DNA polymerases in library amplification.The kit also provides DNA repair enzymes which repair DNA damage such as nicks, gaps, oxidized bases, damaged/blocked 3’ ends, AP sites (apurinic/apyrimidinic sites), and uracil bases. In addition, the DNA repair enzymes significantly increase NGS library yields from FFPE-derived DNA.

Rapid Plus DNA Lib Prep Kit for MGI V2

Rapid Plus DNA Lib Prep Kit for MGI V2

Rapid Plus DNA Lib Prep Kit for MGI V2

Rapid Plus DNA Lib Prep Kit V2 (No DDREs) (Cat. NO RK20271) contains the enzymes and buffers required to convert a broad range of input amounts of DNA into high quality libraries for next-generation sequencing on the Illumina® NGS platforms. Inputs of 1 ng to 1000 ng double-stranded DNA (dsDNA) are required for library preparation. The entire four-step workflow takes place in a single tube or well, in about three hours (Figure 1). No intermediate purification steps or sample transfers are necessary, thus preventing handling errors and loss of valuable samples.
Once purified and quantified, the resulting libraries are ready for Illumina NGS instruments using standard Illumina sequencing reagents and protocols. The kit provides excellent results for high-coverage deep sequencing, such as de novo sequencing, whole genome resequencing, whole exome sequencing, and other enrichment techniques.

Rapid Plus DNA Lib Prep Kit V2 (No DDREs) (Cat. NO RK20271) contains the enzymes and buffers required to convert a broad range of input amounts of DNA into high quality libraries for next-generation sequencing on the Illumina® NGS platforms. Inputs of 1 ng to 1000 ng double-stranded DNA (dsDNA) are required for library preparation. The entire four-step workflow takes place in a single tube or well, in about three hours (Figure 1). No intermediate purification steps or sample transfers are necessary, thus preventing handling errors and loss of valuable samples.
Once purified and quantified, the resulting libraries are ready for Illumina NGS instruments using standard Illumina sequencing reagents and protocols. The kit provides excellent results for high-coverage deep sequencing, such as de novo sequencing, whole genome resequencing, whole exome sequencing, and other enrichment techniques.

Rapid Plus DNA Lib Prep Kit V2 (No DDREs) (Cat. NO RK20271) contains the enzymes and buffers required to convert a broad range of input amounts of DNA into high quality libraries for next-generation sequencing on the Illumina® NGS platforms. Inputs of 1 ng to 1000 ng double-stranded DNA (dsDNA) are required for library preparation. The entire four-step workflow takes place in a single tube or well, in about three hours (Figure 1). No intermediate purification steps or sample transfers are necessary, thus preventing handling errors and loss of valuable samples.
Once purified and quantified, the resulting libraries are ready for Illumina NGS instruments using standard Illumina sequencing reagents and protocols. The kit provides excellent results for high-coverage deep sequencing, such as de novo sequencing, whole genome resequencing, whole exome sequencing, and other enrichment techniques.

Ribonuclease A (RNase A), molecular weight 13.7 kDa, is an endonuclease that specifically acts on the 3′ end of the pyrimidine residue on RNA, cleaving phosphodiester bonds formed with adjacent nucleotides, and the final product of the reaction is pyrimidine 3′ phosphate and an oligonucleotide with pyrimidine 3′ phosphate at the end. RNase A does not work on DNA.This product is sourced from bovine pancreas and is supplied in the form of white lyophilized powder with enzyme activity ≥ 50 Kunitz units/mg protein.
Its recommended working concentration is 1-100 μg/mL, which will vary depending on specific applications.

Ribonuclease A (RNase A), molecular weight 13.7 kDa, is an endonuclease that specifically acts on the 3′ end of the pyrimidine residue on RNA, cleaving phosphodiester bonds formed with adjacent nucleotides, and the final product of the reaction is pyrimidine 3′ phosphate and an oligonucleotide with pyrimidine 3′ phosphate at the end. RNase A does not work on DNA.This product is sourced from bovine pancreas and is supplied in the form of white lyophilized powder with enzyme activity ≥ 50 Kunitz units/mg protein.
Its recommended working concentration is 1-100 μg/mL, which will vary depending on specific applications.

RNase H (Ribonuclease H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. This enzyme does not digest single or double-stranded DNA.

RNase H (Ribonuclease H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. This enzyme does not digest single or double-stranded DNA.

RNase H2 is an endonuclease that preferentially cleaves the 5’ end of ribonucleotides embedded in dsDNA, generating a 5’ phosphate and a 3’ hydroxy terminus. It can also introduce multiple cleavage sites along the RNA portion of the Okazaki fragments.

RNase H2 is an endonuclease that preferentially cleaves the 5’ end of ribonucleotides embedded in dsDNA, generating a 5’ phosphate and a 3’ hydroxy terminus. It can also introduce multiple cleavage sites along the RNA portion of the Okazaki fragments.

RNase Inhibitor, Mammalian specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).
The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 10¹⁴.

RNase Inhibitor, Mammalian specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).
The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 10¹⁴.

RNase Inhibitor, Mammalian has a molecular weight of 50 kD and can efficiently non-covalently bind to RNase A, B, and C in a 1:1 ratio, effectively inhibiting the activity of these enzymes, with a binding constant greater than 10^14. This product does not inhibit the activity of RNase 1, RNase T1, S1 nuclease, RNase H, or RNases derived from the Aspergillus genus. Furthermore, RNase Inhibitor, Mammalian does not inhibit the activity of the following polymerases when used in conjunction: Taq DNA polymerase, AMV or M-MuLV reverse transcriptase, and bacteriophage RNA polymerases (such as SP6, T7, or T3). This product is a high-concentration RNase inhibitor that, when used in conjunction with a glycerol-free dilution solution, can be applied for lyophilized reactions.

RNase Inhibitor, Mammalian has a molecular weight of 50 kD and can efficiently non-covalently bind to RNase A, B, and C in a 1:1 ratio, effectively inhibiting the activity of these enzymes, with a binding constant greater than 10^14. This product does not inhibit the activity of RNase 1, RNase T1, S1 nuclease, RNase H, or RNases derived from the Aspergillus genus. Furthermore, RNase Inhibitor, Mammalian does not inhibit the activity of the following polymerases when used in conjunction: Taq DNA polymerase, AMV or M-MuLV reverse transcriptase, and bacteriophage RNA polymerases (such as SP6, T7, or T3). This product is a high-concentration RNase inhibitor that, when used in conjunction with a glycerol-free dilution solution, can be applied for lyophilized reactions.

rRNA Depletion Kits (Human rRNA & Globin) (ABclonal, Cat. RK30209) is specifically designed for human blood samples to remove rRNA and Globin mRNA from total RNA, suitable for 10-1000 ng total RNA templates.
This kit primarily includes four steps: hybridization of Ribo-Pools probes to target RNA, streptavidin magnetic bead pretreatment, rRNA removal, and RNA purification. The entire procedure can be completed within 60 min without enzymatic digestion reactions and is compatible with high-throughput automation. By utilizing the streptavidin magnetic bead adsorption method, it effectively removes rRNA (including cytoplasmic rRNA and mitochondrial rRNA) and Globin mRNA while preserving other mRNAs and non-coding RNAs for subsequent applications such as whole transcriptome sequencing, long non-coding RNA sequencing, and related research fields.

rRNA Depletion Kits (Human rRNA & Globin) (ABclonal, Cat. RK30209) is specifically designed for human blood samples to remove rRNA and Globin mRNA from total RNA, suitable for 10-1000 ng total RNA templates.
This kit primarily includes four steps: hybridization of Ribo-Pools probes to target RNA, streptavidin magnetic bead pretreatment, rRNA removal, and RNA purification. The entire procedure can be completed within 60 min without enzymatic digestion reactions and is compatible with high-throughput automation. By utilizing the streptavidin magnetic bead adsorption method, it effectively removes rRNA (including cytoplasmic rRNA and mitochondrial rRNA) and Globin mRNA while preserving other mRNAs and non-coding RNAs for subsequent applications such as whole transcriptome sequencing, long non-coding RNA sequencing, and related research fields.

rRNA Depletion Kits (Human rRNA & Globin) (ABclonal, Cat. RK30209) is specifically designed for human blood samples to remove rRNA and Globin mRNA from total RNA, suitable for 10-1000 ng total RNA templates.
This kit primarily includes four steps: hybridization of Ribo-Pools probes to target RNA, streptavidin magnetic bead pretreatment, rRNA removal, and RNA purification. The entire procedure can be completed within 60 min without enzymatic digestion reactions and is compatible with high-throughput automation. By utilizing the streptavidin magnetic bead adsorption method, it effectively removes rRNA (including cytoplasmic rRNA and mitochondrial rRNA) and Globin mRNA while preserving other mRNAs and non-coding RNAs for subsequent applications such as whole transcriptome sequencing, long non-coding RNA sequencing, and related research fields.

rRNA Depletion Kits (Pan-Prokaryote) (ABclonal, Cat. RK30207) is an efficient rRNA removal kit specifically designed for prokaryotes, including bacteria and archaea. This kit is suitable for total RNA samples ranging from 10 to 400 ng and effectively removes 23S, 16S, and 5S rRNA from mixed bacterial samples or single strains using streptavidin magnetic beads.
The kit’s workflow consists of four main steps: hybridization of Ribo-Pools probes with target RNA, pre-treatment with streptavidin magnetic beads, rRNA removal, and RNA purification. The entire process can be completed within 60 minutes without the need for enzymatic digestion and is compatible with high-throughput automation. Since it does not rely on poly A selection, this kit is suitable for the analysis of non-polyadenylated RNA, including non-coding RNA, histones, and RNA sequencing analysis of prokaryotes.

rRNA Depletion Kits (Pan-Prokaryote) (ABclonal, Cat. RK30207) is an efficient rRNA removal kit specifically designed for prokaryotes, including bacteria and archaea. This kit is suitable for total RNA samples ranging from 10 to 400 ng and effectively removes 23S, 16S, and 5S rRNA from mixed bacterial samples or single strains using streptavidin magnetic beads.
The kit’s workflow consists of four main steps: hybridization of Ribo-Pools probes with target RNA, pre-treatment with streptavidin magnetic beads, rRNA removal, and RNA purification. The entire process can be completed within 60 minutes without the need for enzymatic digestion and is compatible with high-throughput automation. Since it does not rely on poly A selection, this kit is suitable for the analysis of non-polyadenylated RNA, including non-coding RNA, histones, and RNA sequencing analysis of prokaryotes.

rRNA Depletion Kits (Pan-Prokaryote) (ABclonal, Cat. RK30207) is an efficient rRNA removal kit specifically designed for prokaryotes, including bacteria and archaea. This kit is suitable for total RNA samples ranging from 10 to 400 ng and effectively removes 23S, 16S, and 5S rRNA from mixed bacterial samples or single strains using streptavidin magnetic beads.
The kit’s workflow consists of four main steps: hybridization of Ribo-Pools probes with target RNA, pre-treatment with streptavidin magnetic beads, rRNA removal, and RNA purification. The entire process can be completed within 60 minutes without the need for enzymatic digestion and is compatible with high-throughput automation. Since it does not rely on poly A selection, this kit is suitable for the analysis of non-polyadenylated RNA, including non-coding RNA, histones, and RNA sequencing analysis of prokaryotes.

rRNA Depletion Kits (Plant) (ABclonal, Cat. RK30208) is a highly efficient rRNA removal kit specifically developed for angiosperms (leaf, seed, and root tissues). This kit is suitable for plant total RNA samples with a template amount of 10-1000 ng, effectively removing cytoplasmic rRNA (including cytoplasmic 5S rRNA, 5.8S rRNA, 18S rRNA, and 28S rRNA), plastid rRNA, and mitochondrial rRNA (including 12S rRNA and 16S rRNA) from total RNA through streptavidin magnetic bead adsorption.
The kit’s workflow consists of four main steps: hybridization of Ribo-Pools probes with target RNA, pre-treatment with streptavidin magnetic beads, rRNA removal, and RNA purification. The entire process can be completed within 60 minutes without the need for enzymatic digestion and is compatible with high-throughput automation. The kit does not rely on poly A selection and can be used for analyzing non-polyadenylated RNA, including non-coding RNA, histones, and whole transcriptome analysis.

rRNA Depletion Kits (Plant) (ABclonal, Cat. RK30208) is a highly efficient rRNA removal kit specifically developed for angiosperms (leaf, seed, and root tissues). This kit is suitable for plant total RNA samples with a template amount of 10-1000 ng, effectively removing cytoplasmic rRNA (including cytoplasmic 5S rRNA, 5.8S rRNA, 18S rRNA, and 28S rRNA), plastid rRNA, and mitochondrial rRNA (including 12S rRNA and 16S rRNA) from total RNA through streptavidin magnetic bead adsorption.
The kit’s workflow consists of four main steps: hybridization of Ribo-Pools probes with target RNA, pre-treatment with streptavidin magnetic beads, rRNA removal, and RNA purification. The entire process can be completed within 60 minutes without the need for enzymatic digestion and is compatible with high-throughput automation. The kit does not rely on poly A selection and can be used for analyzing non-polyadenylated RNA, including non-coding RNA, histones, and whole transcriptome analysis.

rRNA Depletion Kits (Plant) (ABclonal, Cat. RK30208) is a highly efficient rRNA removal kit specifically developed for angiosperms (leaf, seed, and root tissues). This kit is suitable for plant total RNA samples with a template amount of 10-1000 ng, effectively removing cytoplasmic rRNA (including cytoplasmic 5S rRNA, 5.8S rRNA, 18S rRNA, and 28S rRNA), plastid rRNA, and mitochondrial rRNA (including 12S rRNA and 16S rRNA) from total RNA through streptavidin magnetic bead adsorption.
The kit’s workflow consists of four main steps: hybridization of Ribo-Pools probes with target RNA, pre-treatment with streptavidin magnetic beads, rRNA removal, and RNA purification. The entire process can be completed within 60 minutes without the need for enzymatic digestion and is compatible with high-throughput automation. The kit does not rely on poly A selection and can be used for analyzing non-polyadenylated RNA, including non-coding RNA, histones, and whole transcriptome analysis.

The rRNA depletion module (H/M/R) is primarily designed for total RNA samples from humans, mice, and rats. It can effectively remove cytoplasmic rRNA (including cytoplasmic 5S rRNA, 5.8S rRNA, 18S rRNA, and 28S rRNA) and mitochondrial rRNA (including 12S rRNA and 16S rRNA) from total RNA. The resulting RNA can be used for library construction in Whole RNA-seq or other rRNA depletion analyses.
This product is suitable not only for total RNA samples with good integrity but also for degraded RNA samples, such as FFPE RNA. The required starting amount of total RNA is 10 ng to 1 μg.

The rRNA depletion module (H/M/R) is primarily designed for total RNA samples from humans, mice, and rats. It can effectively remove cytoplasmic rRNA (including cytoplasmic 5S rRNA, 5.8S rRNA, 18S rRNA, and 28S rRNA) and mitochondrial rRNA (including 12S rRNA and 16S rRNA) from total RNA. The resulting RNA can be used for library construction in Whole RNA-seq or other rRNA depletion analyses.
This product is suitable not only for total RNA samples with good integrity but also for degraded RNA samples, such as FFPE RNA. The required starting amount of total RNA is 10 ng to 1 μg.

The rRNA depletion module (H/M/R) is primarily designed for total RNA samples from humans, mice, and rats. It can effectively remove cytoplasmic rRNA (including cytoplasmic 5S rRNA, 5.8S rRNA, 18S rRNA, and 28S rRNA) and mitochondrial rRNA (including 12S rRNA and 16S rRNA) from total RNA. The resulting RNA can be used for library construction in Whole RNA-seq or other rRNA depletion analyses.
This product is suitable not only for total RNA samples with good integrity but also for degraded RNA samples, such as FFPE RNA. The required starting amount of total RNA is 10 ng to 1 μg.

Ruby Bst DNA Polymerase is an in silico designed homologue of Geobacillus stearothermophilus DNA Polymerase I(large fragment). Ruby Bst DNA Polymerase has 5´→3´ DNA polymerase activity and strong strand displacement activity but lacks 5´→3´ exonuclease activity. Ruby Bst DNA Polymerase displays improved amplification speed, yield, salt tolerance and thermo stability compared with wild-type Bst DNA Polymerase, Large Fragment.

Ruby Bst DNA Polymerase is an in silico designed homologue of Geobacillus stearothermophilus DNA Polymerase I(large fragment). Ruby Bst DNA Polymerase has 5´→3´ DNA polymerase activity and strong strand displacement activity but lacks 5´→3´ exonuclease activity. Ruby Bst DNA Polymerase displays improved amplification speed, yield, salt tolerance and thermo stability compared with wild-type Bst DNA Polymerase, Large Fragment.