Dry Ice

The most cited neuronal cell culture supplement, B-27 Supplement is an optimized serum-free supplement used to support the low- or high-density growth and short- or long-term viability of embryonic, post-natal, and adult hippocampal and other CNS neurons. B-27 Supplement is provided as a 50X liquid and is intended for use with Neurobasal Medium or Neurobasal-A Medium for neuronal cell culture without the need for an astrocyte feeder layer.

• • Provided as 50X liquid

• • Used with Neurobasal™ Medium for neuronal cell culture

• • Tested in growth assay utilizing rat (Sprague Dawley) embryonic hippocampal neurons

• • Tested also for endotoxin at a 1X concentration

BHI broth is used for the cultivation of a wide variety of microorganisms, including bacteria (streptococci, pneumococci and meningococci), yeasts and molds. It serves as a base for supplemented media containing 0.1% agar, Fildes enrichment or 6.5% sodium chloride.

• To use dehydrated product: suspend 37g in 1L of purified water
• Displays a good cultural response to Neisseria meningitides 13090, Streptococcus pneumonia 6305 and Streptococcus pyogenes 19615
• pH (3.7% solution, 25°C): 7.4 ± 0.2

Certifications

AOAC, BAM, CCAM, CLSI, CMPH2, COMPF, EPA, ISO, MCM9, SMD, SMWW, USDA

• An animal origin (AO) acid hydrolysis of casein
• Less processing results in higher sodium chloride and iron content
• It has successfully be used in the production of a number of animal health vaccines
• Bacto™ Casamino Acids, technical has been shown to support the growth of Bordetella, Haemophilus, and Vibrio species
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

• Bacto Malt Extract is the water-soluble portion of malted barley
• Due to the extraction process, this animal origin-free supplement provides a very high carbohydrate content to any medium
• It has been used successfully in microbial fermentation applications
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

• Bacto Neopeptone is an animal origin (AO) enzymatic digest of bovine (milk) and porcine protein
• This nutritional supplement provides vitamins, nucleotides, minerals, and peptides in a variety of sizes to any culture formulation
• It has been used as a component of culture media in the cultivation of human pathogens, notably Bordetella pertussis and group A streptococci
• Also used with Borrelia burgdorferi and Clostridium species, to produce vaccines for animal health application
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

Applications of Bacto Neopeptone include:

• Recommended for use in media for detection of fungi
• Reported to provide nutrients for support of spirochetes and protozoa
• Microbial fermentation
• Production of vaccines for animal health applications

• Bacto Proteose Peptone No. 3 is a member of the Bacto Proteose Peptones, which are enzymatic digests of protein
• Studies show that no single peptone is the most suitable nitrogen source for every microbiological application
• These findings led to the development of Bacto Proteose Peptone, Bacto Proteose Peptone No. 2, and Bacto Proteose Peptone No. 3. Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone
• Bacto Proteose Peptone No. 3 is used in preparing microbiological and mammalian cell culture media
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

• Bacto Proteose Peptone No. 3 is a member of the Bacto Proteose Peptones, which are enzymatic digests of protein
• Studies show that no single peptone is the most suitable nitrogen source for every microbiological application
• These findings led to the development of Bacto Proteose Peptone, Bacto Proteose Peptone No. 2, and Bacto Proteose Peptone No. 3. Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone
• Bacto Proteose Peptone No. 3 is used in preparing microbiological and mammalian cell culture media
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

• Bacto Proteose Peptone No. 3 is a member of the Bacto Proteose Peptones, which are enzymatic digests of protein
• Studies show that no single peptone is the most suitable nitrogen source for every microbiological application
• These findings led to the development of Bacto Proteose Peptone, Bacto Proteose Peptone No. 2, and Bacto Proteose Peptone No. 3. Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone
• Bacto Proteose Peptone No. 3 is used in preparing microbiological and mammalian cell culture media
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

• Bacto Proteose Peptones are enzymatic digests of protein
• Studies show that no single peptone is the most suitable nitrogen source for every microbiological application
• These findings led to the development of Bacto Proteose Peptone, Bacto Proteose Peptone No. 2, and Bacto Proteose Peptone No. 3. Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone.
• Bacto Proteose Peptone is used in preparing microbiological culture media and in producing bacterial toxins
• Store at 2° to 25°C in cool, dry conditions.

This supplement is a mixture of peptides, amino acids, and carbohydrates, both simple and complex. Customers have successfully used Gibco™ Bacto™ TC Lactalbumin Hydrolysate to support the growth of many mammalian, bacterial, and insect cell types, and it has been used to produce both recombinant proteins and animal health vaccines. Customers have also used this supplement as an amino acid supplement for mammalian cell culture media.

• Bacto Tryptone is an animal origin (AO) pancreatic digest of casein
• It is included in standard methods manual applications and is listed in the Reagent section of the United States Pharmacopeia as meeting the specifications for a pancreatic digest of casein, a component in many of the media listed
• The nutritional profile of this supplement is unique in that it provides a range of nutrients
• Customers have used this supplement in fermentation applications and shown that it successfully supports the growth of many bacterial species during the production of animal health vaccines
• This includes Escherichia coli, and Fusobacterium and Agrobacterium species when used together with Bacto Yeast Extract
• Store in cool, dry conditions in well-sealed containers at 2° to 25°C

Features:

• Pancreatic digest of casein
• Developed while investigating a peptone particularly suitable for the elaboration of indole by bacteria
• Animal origin nutritional supplement

Applications of Bacto Tryptone include:

• Works well in fermentation applications
• Use with common organisms such as Escherichia coli, as well as uncommon organisms such as the diatom Nitzschia laevis

• Bacto Tryptone is an animal origin (AO) pancreatic digest of casein
• It is included in standard methods manual applications and is listed in the Reagent section of the United States Pharmacopeia as meeting the specifications for a pancreatic digest of casein, a component in many of the media listed
• The nutritional profile of this supplement is unique in that it provides a range of nutrients
• Customers have used this supplement in fermentation applications and shown that it successfully supports the growth of many bacterial species during the production of animal health vaccines
• This includes Escherichia coli, and Fusobacterium and Agrobacterium species when used together with Bacto Yeast Extract
• Store in cool, dry conditions in well-sealed containers at 2° to 25°C

Features:

• Pancreatic digest of casein
• Developed while investigating a peptone particularly suitable for the elaboration of indole by bacteria
• Animal origin nutritional supplement

Applications of Bacto Tryptone include:

• Works well in fermentation applications
• Use with common organisms such as Escherichia coli, as well as uncommon organisms such as the diatom Nitzschia laevis

• Bacto Yeast Extracts are concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells, derived from primary grown baker’s yeast
• These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial fermentation, and insect cell culture applications
• Yeast extract provides essential water-soluble vitamins, amino acids, peptides, and carbohydrates to any medium formulation
• Bacto Yeast Extract is one of the most complete and versatile fermentation supplements available
• It is an important ingredient for the microbiological assay of vitamins
• Yeast extract is also of value in the assay of antibiotics
• B factor, a growth substance necessary for the production of rifampin in a Nocardia species, can be isolated from yeast extract
• Bacto Yeast Extract, Technical, was developed to provide products priced for the biotechnology market with acceptable clarity and growth-promoting characteristics
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

Related yeast extract products include:

• Gibco Yeast Extract – Developed to provide a product for the biotechnology/pharmaceutical market with acceptable clarity and growth-promoting characteristics. Media formulations containing yeast extract are specified in standard methods for various applications.
• Difco Yeast Extract UF – Ultra-filtered and specifically designed for mammalian cell culture applications. With its low endotoxin level and high content of naturally occurring B vitamins, it can be used effectively to reduce the need for fetal bovine serum. It has an endotoxin level of less than or equal to 500 EU/g
• Difco Yeast Extract Low-Dusting (LD) – Designed to limit the amount of dust generated compared to other spray-dried yeast extracts.

• Bacto Yeast Extracts are concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells, derived from primary grown baker’s yeast
• These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial fermentation, and insect cell culture applications
• Yeast extract provides essential water-soluble vitamins, amino acids, peptides, and carbohydrates to any medium formulation
• Bacto Yeast Extract is one of the most complete and versatile fermentation supplements available
• It is an important ingredient for the microbiological assay of vitamins
• Yeast extract is also of value in the assay of antibiotics
• B factor, a growth substance necessary for the production of rifampin in a Nocardia species, can be isolated from yeast extract
• Bacto Yeast Extract, Technical, was developed to provide products priced for the biotechnology market with acceptable clarity and growth-promoting characteristics
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

Related yeast extract products include:

• Gibco Yeast Extract – Developed to provide a product for the biotechnology/pharmaceutical market with acceptable clarity and growth-promoting characteristics. Media formulations containing yeast extract are specified in standard methods for various applications.
• Difco Yeast Extract UF – Ultra-filtered and specifically designed for mammalian cell culture applications. With its low endotoxin level and high content of naturally occurring B vitamins, it can be used effectively to reduce the need for fetal bovine serum. It has an endotoxin level of less than or equal to 500 EU/g
• Difco Yeast Extract Low-Dusting (LD) – Designed to limit the amount of dust generated compared to other spray-dried yeast extracts.

• Bacto Yeast Extracts are concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells, derived from primary grown baker’s yeast
• These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial fermentation, and insect cell culture applications
• Yeast extract provides essential water-soluble vitamins, amino acids, peptides, and carbohydrates to any medium formulation
• Bacto Yeast Extract is one of the most complete and versatile fermentation supplements available
• It is an important ingredient for the microbiological assay of vitamins
• Yeast extract is also of value in the assay of antibiotics
• B factor, a growth substance necessary for the production of rifampin in a Nocardia species, can be isolated from yeast extract
• Bacto Yeast Extract, Technical, was developed to provide products priced for the biotechnology market with acceptable clarity and growth-promoting characteristics
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

Related yeast extract products include:

• Gibco Yeast Extract – Developed to provide a product for the biotechnology/pharmaceutical market with acceptable clarity and growth-promoting characteristics. Media formulations containing yeast extract are specified in standard methods for various applications.
• Difco Yeast Extract UF – Ultra-filtered and specifically designed for mammalian cell culture applications. With its low endotoxin level and high content of naturally occurring B vitamins, it can be used effectively to reduce the need for fetal bovine serum. It has an endotoxin level of less than or equal to 500 EU/g
• Difco Yeast Extract Low-Dusting (LD) – Designed to limit the amount of dust generated compared to other spray-dried yeast extracts

• Concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells derived from primary grown baker’s yeast
• These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial fermentation, and insect cell culture applications
• Yeast extract provides essential water-soluble vitamins, amino acids, peptides, and carbohydrates to any medium formulation
• Bacto Yeast Extract is an important ingredient for the microbiological assay of vitamins
• B factor, a growth substance necessary for the production of rifampin in a Nocardia species, can be isolated from yeast extract
• Developed to provide products priced for the biotechnology market with acceptable clarity and growth-promoting characteristics
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

The combination of pancreatic digest of casein and yeast extract provides synergistic nutritional benefits that are not provided by the components alone, resulting in a unique nutritional profile. Customers have shown the combined use of these two peptones, when supplied as Biosate Peptone, improves toxin production with Clostridial species for vaccines, such as Clostridium botulinum and Clostridium perfringens.

Gibco™ BiTek™ Fermentation Broth is a bacterial fermentation formulated with ingredients that exhibit superior consistency, performance, and solubility.

Developed while investigating a peptone particularly suitable for the elaboration of indole by bacteria. The final product goes through fewer refinement steps during processing than Gibco™ Bacto™ Tryptone.

• Prepared according to the method described by Mueller and Miller
• Supplemented with growth factors, cystine, maltose and iron and inorganic salts
• Successful in fermentation for Pneumococcal, Diptheria, Pertussis, Tetanus and Zoster vaccines
• Support the growth of Bordetella, Corynebacterium, Pseudomonas, Haemophilus and Escherichia
• Dehydrated format with Peptones / Hydrolysates
• DNR (Latex) free

• Prepared according to the method described by Mueller and Miller
• Supplemented with growth factors, cystine, maltose and iron and inorganic salts
• Successful in fermentation for Pneumococcal, Diptheria, Pertussis, Tetanus and Zoster vaccines
• Support the growth of Bordetella, Corynebacterium, Pseudomonas, Haemophilus and Escherichia
• Dehydrated format with Peptones / Hydrolysates
• DNR (Latex) free

A complete media used in the cultivation of microorganisms from the class Mollicutes. Used in animal health applications for the propagation of Mycoplasma species such as Mycoplasma mycoides, Mycoplasma gallisepticum, and others for vaccine production.

• An alternative carbohydrate profile to yeast supplements for mammalian cell processes, such as CHO
• High concentrations of vitamins and carbohydrates
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

Difco Tryptone Peptone is an animal origin (AO) pancreatic digest of casein. In addition to providing a unique nutritional profile, it has a higher nitrogen content than our other casein products. Tryptone Peptone supports the growth of many different types of bacteria and fungi, including fastidious organisms and in toxin production. Customers have used this supplement in the development of animal health vaccines, such as Clostridial vaccines, and with human health Botox-like products produced from Clostridium botulinum.

Features:
• Animal origin, pancreatic digest of casein
• Primary nitrogen source in trypticase soy broth and agar
• Referenced in Official Methods of Analysis of AOAC International
• Supports the growth of many different types of bacteria and fungi

Applications of Tryptone Peptone include:
• Recommended for use in media formulations when good growth of fungi and bacteria is required
• Used in the development of animal health vaccines and with human health Botox-like products

L-Glutamine is an amino acid that is required for cell culture. L-Glutamine participates in the formation of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate, and other amino acids, as well as in protein synthesis and glucose production. Unlike most other amino acids, L-glutamine is not stable in solution. The rate at which degradation proceeds is a function of time, temperature, and pH.

• • Optimal concentration is dependent upon the cell type and medium used to culture the cells, but generally falls in the range of 2 to 6 mM

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• • Manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK, registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard

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Bioproduction, Cell Culture, Cloning, Mammalian Cell Culture, Transformation, Viral Vaccine Production.

Neurobasal Plus Medium is a neuronal basal medium that provides the highest rates of in vitro survival of primary rodent and human stem cell-derived neurons when used with B-27 Plus Supplement. Neurobasal Plus Medium features an optimized formulation, upgraded manufacturing process, and more stringent quality control of raw materials and final product. These improvements enable the highest rates of neuronal cell survival, improved electrophysiological activity, and enhanced functional maturity versus other neuronal cell culture media systems.nnNeurobasal Plus Medium, when combined with B-27 Plus Supplement, offers:

• • At least 50% higher survival rate of primary rat neurons compared to classic Neurobasal Medium and B-27 Supplement

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• • Highest survival rate of primary rat, mouse, and human stem cell-derived neurons compared to other commercially available serum-free neuronal media systems

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• • Improved electrophysiological activity and maturation—more consistent, stable, and synchronized spontaneous activity in primary rodent neurons after 10 days compared to BrainPhys™ Neuronal Medium

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• • Accelerates neurite outgrowth and increases the number of synaptic-positive puncta

• An animal origin-free (AOF) enzymatic digest of soy
• A nutritious and excellent carbohydrate source due to the naturally high concentrations of vitamins and carbohydrates in soy
• Customers have successfully used this supplement in CHO-based production of monoclonal antibodies for oncological and rheumatological therapeutics
• Also used with many different microbial cell types to produce animal health vaccines for swine, such as Clostridial vaccines

Applications of Phytone Peptone include:

• It is an excellent plant peptone for the cultivation of fungi and fastidious types of bacteria, such as members of the Clostridium and Neisseria genera
• It has been used in mammalian cell culture applications due to its high carbohydrate content

For many years, researchers have used a common recipe for making freezing medium: High-glucose Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% serum and 10% DMSO. Recovery Cell Culture Freezing Medium is an optimized version of this classic formulation, using Gibco Bovine Serum and Fetal Bovine Serum to provide improved cell recovery and viability after thawing.

• • Suitable for a wide variety of mammalian cells

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• • Can be used to freeze most mammalian cells cultured in DMEM, DMEM/F-12, MEM, RPMI 1640, and Ham’s F-12

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• • Quality tested for pH, osmolality, sterility, and endotoxin

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• • Each lot is performance tested using CHO-K1 cells

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Cell Counting, Viability & Cryopreservation, Cell Culture, Mammalian Cell Culture, Gene Expression Analysis & Genotyping, In Vitro Transcription, PCR & Real-Time PCR