Dry Ice

A chemically defined supplement formulation to complement a variety of base media. This supplement was developed using several host cell lines and in various culture systems, including shaker flasks and bench-scale bioreactors. Developed for use in CHO mammalian culture systems and can be used either as a media supplement or a culture system feed.

A chemically defined supplement formulation to complement a variety of base media. This supplement was developed using several host cell lines and in various culture systems, including shaker flasks and bench-scale bioreactors. Developed for use in CHO mammalian culture systems and can be used either as a media supplement or a culture system feed.

An animal origin-free (AOF) protein-free chemically defined supplement. It can be used in batch and fed batch mode in cell culture processes. All components are chemically defined with known molecular formulae and structures and meet our stringent Animal Origin-Free (AOF) specification. Formulated without glucose, L-glutamine, and sodium bicarbonate. Due to the balanced nutritional nature of this supplement, rich in amino acids, it has also been used in bacterial applications.

A chemically defined supplement formulation to complement a variety of base media. This supplement was developed using several host cell lines and in various culture systems, including shaker flasks and bench-scale bioreactors. Developed for use in CHO mammalian culture systems and can be used either as a media supplement or a culture system feed.

Sera for cell culture with robust cell lines
• Good value for basic research
• Endotoxin level: = 20 EU/mL (levels routinely = 10 EU/mL)
• Hemoglobin level: = 25 mg/dL
• Origin: Refer to CoA for specific origin (US, Canada, Mexico, Brazil, Australia, New Zealand)n

Gibco serum delivers:
• ISO 13485-certified, processed in FDA-registered facilities
• Triple filtered at 0.1 µm
• Gibco bottle is easier to use in hood, reduces risk of contamination, and helps you perform cell culture more consistently
• Peel-off sticker on label provides handy reference for lot number and expiration date recording in lab notebook

• Routinely used for attachment and maintenance of human embryonic stem cells (hESC)
• Human ESCs have specific needs for attachment and maintenance and some lots of basement membrane extract may not provide adequate support
• Geltrex™ hESC-qualified reduced growth factor basement membrane matrix has been tested for support of hESC growth and pluripotency, therefore minimizing the need to test multiple lots
• Consistent manufacturing processes help ensure a protein concentration averaging 15 mg/mL with very little variation from lot-to-lot

RevitaCell™ Supplement (100X) has been optimized for use with pluripotent stem cells (PSCs), either as a post-thaw recovery solution to improve cell viability or in combination with Essential 8™ Medium to enhance single cell passaging applications. It can also be used for recovery of primary neurons, traditionally a challenging cell to efficiently recover. The significant improvement seen in these applications is due to the presence of a more specific ROCK inhibitor coupled with molecules containing antioxidant and free radical scavenger properties that minimize the impact of stress on the cells.

• • Enabling—provides maximum cell viability when performing single cell passaging

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• • Rapid cell recovery—high post-thaw cell viability and growth of pluripotent stem cells (passaged as clumps or single cells) and neurons

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• • Consistent—formulated using animal origin-free and chemically defined materials for quality and confidence
    
    Single cell passaging
    Use of the RevitaCellTM Supplement for 18-24 hours post-single cell passaging provides vast improvement in cell survival. Cells are shown to reach passaging confluency within 3-4 days post-seeding.
    
    Higher specificity
    Kinase profiling of pro-survival small molecules in RevitaCell™ Supplement shows higher specificity in comparison to traditional ROCK inhibitors (i.e., Y-27632 and Thiazovivin).
    
    Quicker post-thaw recovery
    PSCs recovered at 40,000 viable cells/cm² using media supplemented with RevitaCell™ Supplement for the first 24 hours post-thaw recovery followed by feeding with unsupplemented Essential 8™ Medium will typically reach 80% confluency in <5 days, significantly reducing the average post-thaw recovery time of most cryopreservation solutions. Neurite outgrowth of neurons is also significantly improved using RevitaCell™ Supplement.

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Glucagon

Glucose Oxidase

Glucose Oxidase

Glucose Oxidase

Glucose Oxidase

Glucose-6-Phosphate Dehydrogenase

Glucose-6-Phosphate Dehydrogenase

Glucose-6-Phosphate Dehydrogenase

Glutamic Pyruvic Transaminase

Glutamic Pyruvic Transaminase

Glycerol-3-Phosphate Oxidase

Glycerol-3-Phosphate Oxidase

Glycerol-3-Phosphate Oxidase

Glycogen is an inert material and does not interfere with molecular biology experiments. It can replace tRNAs or sonicated DNAs as an additive for the precipitation of nucleic acids.

This serum is produced from whole blood collected from healthy adult animals.

This serum is produced from whole blood collected from healthy adult animals.

For use in immunohistochemistry (IHC) and cell culture. Goat serum is a suitable replacement for FBS on most cell lines and primary cultures for Biomedical Research – virus isolation and identification, virological studies, etc.

For use in immunohistochemistry (IHC) and cell culture. Goat serum is a suitable replacement for FBS on most cell lines and primary cultures for Biomedical Research – virus isolation and identification, virological studies, etc.

Goat Serum is used as a supplement to cell culture media. Goat Serum provides a broad spectrum of macromolecules, carrier proteins for lipoid substances and trace elements, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors that promote cell growth and health. Be certain to maintain Good Cell Culture Practice, and maintain sterility of cultures that require media supplementation.

Goat Serum is used as a supplement to cell culture media. Goat Serum provides a broad spectrum of macromolecules, carrier proteins for lipoid substances and trace elements, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors that promote cell growth and health. Be certain to maintain Good Cell Culture Practice, and maintain sterility of cultures that require media supplementation.