Dry Ice

5’…G G T C T C(N)₁…3′
3’…C C A G A G(N)₅…5′

5’…G/ A A T T C…3′
3’…C T T A A/ G…5′

5’…G/ A A T T C…3′
3’…C T T A A/ G…5′

5’…GAT/ATC…3′
3’…CTA/TAG…5′
Isoschizomers: Eco32I

5’…GAT/ATC…3′
3’…CTA/TAG…5′
Isoschizomers: Eco32I

Endonuclease III (Nth) protein acts as both N-glycosylase and a AP-lyase.The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA, generating a basic (AP site). The AP-lyase activity of the enzyme cleaves 3´ to the AP site leaving a 5´ phosphate and a 3´ ring opened sugar.

Endonuclease IV derived from E. coli recognizes the depurine/depyrimidine (AP) site on the double- stranded DNA molecule, and cleaves the first phosphodiester bond at the 5′ ́end of the AP site to form 3′-OH and 5’dRP . In addition, Endonuclease IV has 3’́diesterase activity, which releases phosphoglyceraldehyde, intact deoxyribose 5′ -phosphate, and phosphoric acid from the 3′ ́end of DNA. The best substrate for this enzyme is AP double-stranded DNA, but it is also active against AP single-stranded DNA.

Endonuclease IV derived from E. coli recognizes the depurine/depyrimidine (AP) site on the double- stranded DNA molecule, and cleaves the first phosphodiester bond at the 5′ ́end of the AP site to form 3′-OH and 5’dRP . In addition, Endonuclease IV has 3’́diesterase activity, which releases phosphoglyceraldehyde, intact deoxyribose 5′ -phosphate, and phosphoric acid from the 3′ ́end of DNA. The best substrate for this enzyme is AP double-stranded DNA, but it is also active against AP single-stranded DNA.

Endonuclease VIII originates from E.coli and possesses both N-glycosylase and AP-endonuclease activity. The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA, generating an apurinic (AP site). The AP-endonuclease the phosphodiester bond at the AP site, forming 3’-P and 5’-P ends.
Damaged bases recognized and removed by Endonuclease VIII include: urea, 5, 6-dihydroxythymine, thymine glycol, 5-hydroxy-5-methylhyd antoin, uracil glycol, 6-hydroxy-5, 6-dihydrothymine and methylhydroxy- methyluracil.

Endonuclease VIII originates from E.coli and possesses both N-glycosylase and AP-endonuclease activity. The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA, generating an apurinic (AP site). The AP-endonuclease the phosphodiester bond at the AP site, forming 3’-P and 5’-P ends.
Damaged bases recognized and removed by Endonuclease VIII include: urea, 5, 6-dihydroxythymine, thymine glycol, 5-hydroxy-5-methylhyd antoin, uracil glycol, 6-hydroxy-5, 6-dihydrothymine and methylhydroxy- methyluracil.

Enterokinase

Enterokinase

Entrans 2X qPCR Probe Master Mix is a special reagent for qPCR reactions based on the primer-probe detection. When a probe is bound to the template DNA strand, the 5′-3 ‘exonuclease activity of Taq enzyme can be used to hydrolyze the probe, thereby releasing fluorescent signal. This product uses chemically modified hot-start Taq enzyme for amplification. Accurate quantification, high amplification efficiency, good repeatability and wide credibility. Entrans 2X qPCR Probe Master Mix is a 2X concentration master mix, which contains all the components required for qPCR except primers, probes and templates.

Entrans 2X qPCR Probe Master Mix is a special reagent for qPCR reactions based on the primer-probe detection. When a probe is bound to the template DNA strand, the 5′-3 ‘exonuclease activity of Taq enzyme can be used to hydrolyze the probe, thereby releasing fluorescent signal. This product uses chemically modified hot-start Taq enzyme for amplification. Accurate quantification, high amplification efficiency, good repeatability and wide credibility. Entrans 2X qPCR Probe Master Mix is a 2X concentration master mix, which contains all the components required for qPCR except primers, probes and templates.

Entrans 2X qPCR Probe Master Mix V2 is a premixed reagent for probe-based qPCR reactions, containing all components except primers, probes and templates. This master mix includes Hot start Taq DNA polymerase modified both chemically and by antibody to inhibit non-specific amplification, which can guarantee high efficiency, sensitivity，and also high reproducibility in qPCR amplification. The optimization of the buffer system allows the product to perform multiple fluorescence quantitative experiments, and it is compatible with different species and provides a powerful tool for multi-disciplinary experimental research.

Entrans 2X qPCR Probe Master Mix V2 is a premixed reagent for probe-based qPCR reactions, containing all components except primers, probes and templates. This master mix includes Hot start Taq DNA polymerase modified both chemically and by antibody to inhibit non-specific amplification, which can guarantee high efficiency, sensitivity，and also high reproducibility in qPCR amplification. The optimization of the buffer system allows the product to perform multiple fluorescence quantitative experiments, and it is compatible with different species and provides a powerful tool for multi-disciplinary experimental research.

Entrans 2X qPCR Probe Master Mix with UDG is aready-to-use reagent for probe-based qPCRreactions, containing all components exceptprimers, probes and templates. This master mixincludes a chemically modified version of Taqpolymerase with Hot-start capability to inhibit non specific amplification, while allowing high efficiency,sensitivity and reproducibility in qPCR amplification.At the same time, and has joined the UDG anti pollution system. The optimization of the Buffersystem allows the product to perform multiplefluorescence quantitative experiments, andIt is suitable for multiple species and provides apowerful tool for multi-disciplinary experimentalneeds.

Entrans 2X qPCR Probe Master Mix with UDG is aready-to-use reagent for probe-based qPCRreactions, containing all components exceptprimers, probes and templates. This master mixincludes a chemically modified version of Taqpolymerase with Hot-start capability to inhibit non specific amplification, while allowing high efficiency,sensitivity and reproducibility in qPCR amplification.At the same time, and has joined the UDG anti pollution system. The optimization of the Buffersystem allows the product to perform multiplefluorescence quantitative experiments, andIt is suitable for multiple species and provides apowerful tool for multi-disciplinary experimentalneeds.

Entrans 2X SNP Genotyping qPCR Probe Master Mix (with UDG)

Entrans 2X SNP Genotyping qPCR Probe Master Mix (with UDG)

Entrans qPCR Probe Kit is designed for probe-based qPCR reactions. It contains all components required for a rapid and reliable qPCR reaction except primers, probes and templates. When an intact probe binds to the complementary template DNA strand, the reporter dye and quencher are in proximity and the quencher absorbs the fluorescence emitted by the reporter dye. In PCR extension, the 5 ‘-3 ‘exonuclease activity of Taq polymerase cleaves the hybridized probe, separating the reporter from the quencher and releasing fluorescent signal. This kit includes an engineered version of Taq polymerase with antibody-mediated Hot-start capability to inhibit non-specific amplification at room temperature, while allowing high efficiency, sensitivity and reproducibility in qPCR amplification. The Entrans qPCR Probe Kit is an ideal product for high-speed multiplex qPCR analyses.

Entrans qPCR Probe Kit is designed for probe-based qPCR reactions. It contains all components required for a rapid and reliable qPCR reaction except primers, probes and templates. When an intact probe binds to the complementary template DNA strand, the reporter dye and quencher are in proximity and the quencher absorbs the fluorescence emitted by the reporter dye. In PCR extension, the 5 ‘-3 ‘exonuclease activity of Taq polymerase cleaves the hybridized probe, separating the reporter from the quencher and releasing fluorescent signal. This kit includes an engineered version of Taq polymerase with antibody-mediated Hot-start capability to inhibit non-specific amplification at room temperature, while allowing high efficiency, sensitivity and reproducibility in qPCR amplification. The Entrans qPCR Probe Kit is an ideal product for high-speed multiplex qPCR analyses.

This product utilizes HS Taq DNA Polymerase as the core enzyme and adopts a dual modification method with chemical antibody and modification, aiming to ensure amplification efficiency while significantly enhancing the specificity of the product, suitable for single nucleotide polymorphism (SNP) genotyping detection.

This product utilizes HS Taq DNA Polymerase as the core enzyme and adopts a dual modification method with chemical antibody and modification, aiming to ensure amplification efficiency while significantly enhancing the specificity of the product, suitable for single nucleotide polymorphism (SNP) genotyping detection.

Exonuclease I catalyzes the removal of nucleotides from single-stranded DNA in the 3′ to 5′ direction, and does not degrade DNA strands with the 3′-OH end blocked by phosphoryl or acetyl groups. Exonuclease I has strict substrate specificity and is incapable of degrading double-stranded DNA and RNA.

Exonuclease I catalyzes the removal of nucleotides from single-stranded DNA in the 3′ to 5′ direction, and does not degrade DNA strands with the 3′-OH end blocked by phosphoryl or acetyl groups. Exonuclease I has strict substrate specificity and is incapable of degrading double-stranded DNA and RNA.

Exonuclease III (E.coli) catalyzes the stepwise removal of mononucleotides from 3´-hydroxyl termini of duplex DNA. The preferred substrates are dsDNA with blunt or recessed 3´-, termini although the enzyme also acts at nicks in dsDNA to produce single-strand gaps. dsDNA with 3´-overhang are resistant to cleavage; when overhang’s length is ≥ 4 bases，dsDNA are able to fully prevent ExoIII cleavage process.
Exonuclease III has also been reported to have RNase H, 3´-phosphatase and AP-endonuclease activities.

Exonuclease III (E.coli) catalyzes the stepwise removal of mononucleotides from 3´-hydroxyl termini of duplex DNA. The preferred substrates are dsDNA with blunt or recessed 3´-, termini although the enzyme also acts at nicks in dsDNA to produce single-strand gaps. dsDNA with 3´-overhang are resistant to cleavage; when overhang’s length is ≥ 4 bases，dsDNA are able to fully prevent ExoIII cleavage process.
Exonuclease III has also been reported to have RNase H, 3´-phosphatase and AP-endonuclease activities.

Exonuclease T is a single-strand-specific exonuclease that catalyzes the degradation of linear single-stranded DNA or RNA in the 3′ ́to 5′ direction.

Exonuclease T is a single-strand-specific exonuclease that catalyzes the degradation of linear single-stranded DNA or RNA in the 3′ ́to 5′ direction.

Exonuclease VII (Exo VII) can cleave single-stranded DNA in both 5′-3′ and 3′-5′ directions and is inactive against linear or circular double-stranded DNA. When PCR requires stepwise completion to obtain the final target product, this enzyme can be used to remove residual oligonucleotide primers left during intermediate stages of PCR, eliminating interference with subsequent use of different primers. The enzyme’s digestion of single-stranded DNA is independent of metal ions. This product is a highly purified protein obtained through the recombinant expression of the Exonuclease VII gene (XseA and XseB).

Exonuclease VII (Exo VII) can cleave single-stranded DNA in both 5′-3′ and 3′-5′ directions and is inactive against linear or circular double-stranded DNA. When PCR requires stepwise completion to obtain the final target product, this enzyme can be used to remove residual oligonucleotide primers left during intermediate stages of PCR, eliminating interference with subsequent use of different primers. The enzyme’s digestion of single-stranded DNA is independent of metal ions. This product is a highly purified protein obtained through the recombinant expression of the Exonuclease VII gene (XseA and XseB).

The Fast One Step Probe RT-qPCR Kit is a reagent kit designed for rapid amplification quantitative RT-qPCR detection using the probe method. It includes reaction buffer, hot-start Taq polymerase, novel reverse transcriptase ABScript Ⅲ Reverse Transcriptase, and heat-labile UDG. This kit utilizes RNA as a template and includes gene-specific primers, enabling consecutive reverse transcription and PCR reactions to take place in the same tube without the requirement for additional pipetting or tube opening steps. This streamlined process significantly enhances throughput. The kit utilizes dUTP/UDG to prevent contamination, with the heat-sensitive UDG rapidly degrading uracil-containing contaminants at room temperature. At 50°C during reverse transcription, the heat-labile UDG quickly deactivates, ensuring it does not affect the efficiency and sensitivity of RT-qPCR.

The Fast One Step Probe RT-qPCR Kit is a reagent kit designed for rapid amplification quantitative RT-qPCR detection using the probe method. It includes reaction buffer, hot-start Taq polymerase, novel reverse transcriptase ABScript Ⅲ Reverse Transcriptase, and heat-labile UDG. This kit utilizes RNA as a template and includes gene-specific primers, enabling consecutive reverse transcription and PCR reactions to take place in the same tube without the requirement for additional pipetting or tube opening steps. This streamlined process significantly enhances throughput. The kit utilizes dUTP/UDG to prevent contamination, with the heat-sensitive UDG rapidly degrading uracil-containing contaminants at room temperature. At 50°C during reverse transcription, the heat-labile UDG quickly deactivates, ensuring it does not affect the efficiency and sensitivity of RT-qPCR.

Fast RNA-seq Lib Prep Kit V2

Fast RNA-seq Lib Prep Kit V2

Fast RNA-seq Lib Prep Kit V2