Wet Ice

MP Biomedicals Ultra-Sense Femto-Plus Western ECL Substrate possesses the following highlights: (1) No protocol optimization is required. (2) Easy-to-Use, just mix the solution A and B (1:1) for 30 seconds prior to use. (3) High degree of sensitivity and enhanced chemiluminescence for femtogram detection of protein. (4) Optimized for use with PVDF and nitrocellulose membranes. (5) Compatible with Western Blotting Markers. (6) Optimized for film- and CCD-based imaging.

• • Binds the spherical particles for use as a single-color compensation controls.

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• • Binds with mouse, rat and hamster antibodies, but also bind with rabbit and recombinant human antibodies.

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• • Designed for use in compensation with all fluorochromes excited by ultraviolet (355 nm) violet (405 nm), blue (488 nm), green (532 nm), yellow-green (561 nm), and red (633-640 nm) lasers.

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• • Compensate in a manner similar to cells when used with Brilliant Violet 785/786-, Brilliant Violet 711-, Super Bright 780- and Super Bright 702-conjugated antibodies.

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• • Multispecies compatibility—bind with mouse, rat, hamster, rabbit and recombinant human antibodies

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• • Improved fluorophore compatibility

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• • Each drop of beads contains two populations: a positive population that bind with any mouse, rat, hamster, rabbit or recombinant human antibody and a negative population that will not bind with antibody.

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• • When a fluorochrome-conjugated antibody is added to the beads, both positive and negative populations are observed.

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• • This bimodal distribution can be used for single-color compensation controls to accurately setup instrumentation for multicolor flow cytometry experiments.

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