Wet Ice

The AFC substrates have both chromogenic (E380 = 12600M-1Cm-1) and fluorogenic (Excitation 400 nm and Emission 505 nm) characteristics. The specificity and sensitivity of AFC allow for the detection of enzyme activities in cell lysate or electrophoretic gels

Z-ASP(OME)-GLN-MET-ASP(OME)-FLUOROMETHYLKETONE

Z-Asp(Ome)-Glu(Ome)-Val-Asp(Ome)-Fluoromethylketone

Z-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-fluoromethylketone

Z-Asp(OME)-Glu(OME)-Val-Asp(OME)-Fluoromethylketone

Z-Glu-Pro-Arg-7-Amino-4-Methylcoumarin Z

Z-Glu-Pro-Arg-7-Amino-4-Methylcoumarin Z

Z-Gly-Arg-Thiobenzyl Ester

Z-Gly-Arg-Thiobenzyl Ester

Z-Gly-Leu-Phe-CMK

Z-Gly-Leu-Phe-CMK

Z-Gly-Pro-Arg-7-Amino-4-Trifluoromethylcoumarin

Z-Gly-Pro-Arg-7-Amino-4-Trifluoromethylcoumarin

Z-ILE-GLU(OME)-THR-ASP(OME)-FLUOROMETHYLKETONE

Z-ILE-GLU(OME)-THR-ASP(OME)-FLUOROMETHYLKETONE

Z-ILE-GLU(OME)-THR-ASP(OME)-FLUOROMETHYLKETONE

Z-Leu-Glu(Ome)-His-Asp(Ome)-Fluoromethylketone

Z-Leu-Glu(OMe)-His-Asp(OMe)-fluoromethylketone

Z-Leu-Glu(OMe)-His-Asp(OMe)-fluoromethylketone

Z-Leu-Leu-Tyr-Fluoromethylketone

Z-Leu-Leu-Tyr-fluoromethylketone

Z-Leu-Leu-Tyr-fluoromethylketone

Z-PHE-ALA-FLUOROMETHYLKETONE

Z-PHE-ALA-FLUOROMETHYLKETONE

Z-PHE-ALA-FLUOROMETHYLKETONE

Z-Phe-Phe-Fluoromethylketone

Z-Phe-Phe-Fluoromethylketone

Z-Phe-Phe-Fluoromethyl-Ketone

Z-TYR-VAL-ALA-ASP-AFC

Z-TYR-VAL-ALA-ASP-AFC

Z-Tyr-Val-Ala-Asp(Ome)-Fluoromethylketone

Z-Val-Ala-Asp-(Ome)-Fluoromethylketone

Z-Val-Ala-Asp-(Ome)-Fluoromethylketone

Z-Val-Ala-Asp-(Ome)-Fluoromethylketone

Z-Val-Ala-Asp-Fluoromethylketone

Z-Val-Ala-Asp-Fluoromethylketone

Z-Val-Ala-Asp-Fluoromethylketone

Z-Val-Asp(Ome)-Val-Ala-Asp(Ome)-Fluoromethylketone

Z-Val-Asp(OMe)-Val-Ala-Asp(OMe)-fluoromethylketone

Z-Val-Asp(OMe)-Val-Ala-Asp(OMe)-fluoromethylketone

Z-Val-Glu(Ome)-Ile-Asp(Ome)-Fluoromethylketone

Z-Val-Glu(OMe)-Ile-Asp(OMe)-fluoromethylketone

Z-Val-Glu(OMe)-Ile-Asp(OMe)-fluoromethylketone

Z-Val-Leu-Arg-AMC

Z-Val-Leu-Arg-AMC