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Description

His Tag Antibody Plate is a microtiter plate coated with THE^TM Anti His Tag Antibody. The plate is designed for capturing His tagged protein with His tag located at N-terminus, C-terminus, or internal of the protein. It can be applied for analysis of different length of His tag including Hexa-His (6 His), Penta-His (5-His) and Tetra-His (4-His).The plate demonstrate high sensitivity and high binding capacity. The capacity and sensitivity varies, depending on protein size, structure and solution buffer. Generally, proteins with lower molecular weight (M.W) can be more sensitive to the His antibody plate than ones with higher M.W. Compared with Nickel Coated Plate, the affinity plate can bind target proteins with high specificity, and tolerable with several common used reagents such as EDTA, imidazole and β-ME.

Description

His Tag Antibody Plate is a microtiter plate coated with THE^TM Anti His Tag Antibody in a non-removable format. The plate is designed for capturing His tagged protein with His tag located at N-terminus, C-terminus, or internal of the protein. It can be applied for analysis of different length of His tag including Hexa-His (6 His), Penta-His (5-His) and Tetra-His (4-His).The plate demonstrate high sensitivity and high binding capacity. The capacity and sensitivity varies, depending on protein size, structure and solution buffer. Generally, proteins with lower molecular weight (M.W) can be more sensitive to the His antibody plate than ones with higher M.W. Compared with Nickel Coated Plate, the affinity plate can bind target proteins with high specificity, and tolerable with several common used reagents such as EDTA, imidazole and 2-ME.

GenScript His Tag ELISA Detection Kit, a 1.5 hour competition ELISA, is developed for rapid and high throughput detection of His-tagged
proteins. There are several potential usages:

1. Quickly identify the presence of His-tagged proteins in samples.
2. Optimize protein expression by monitoring the His-tagged proteins level.
3. High throughput screening of stable cell lines expressing His-tagged proteins.

This kit is based on competitive ELISA method. His Tag Plate in the kit is a 96-well microtiter
plate coated with a His-tagged protein with the molecular weight of 12.7 kDa. The
plate, which comprises of 8 wells x 12 strips, is demountable. When Anti-His Monoclonal
Antibody and His-tagged proteins are added to the well , the coated His-tagged
proteins compete with His Tag Standard (His-tagged protein with the molecular weight of 11.3
kDa) in solution or His-tagged
protein in sample to interact with Anti-His Monoclonal Antibody. The higher the
concentration of the His-tagged protein in solution is, the less the antibody
bound to the plate will be. The Anti-His Monoclonal Antibody used in the kit is
a mouse anti His tag monoclonal antibody (GenScript A00186). Antibody Tracer, which is a horseradish peroxidase (HRP) conjugated Goat anti mouse IgG, is used for enzyme reaction. His-tagged protein, Anti-His Monoclonal Antibody and antibody tracer form a complex. Other unbound molecules can be removed by washing
solution. The antibody tracer reacts with TMB substrate to develop blue
product that turns yellow immediately when the Stop Solution is added, which
can be measured by microplate reader at 450 nM.

In optimized
test condition, each absorbance value is indicated to the individual His-tagged
proteins amount in solution.  His-tagged
protein standards of known concentration and the corresponding absorbance
values are used to construct a standard curve. With the standard curve, His-tagged
protein amount present in the unknown sample can be calculated by transforming
its absorbance value. 

GenScript His-tag antibody [Biotin], pAb, Rabbit specifically reacts with fusion proteins containing His tagged fusion proteins.

GenScript His-tag Antibody, pAb, Rabbit specifically reacts with fusion proteins containing His epitope tags.For N-terminal His-tagged protein detection, THE™ Anti-His mAb (Cat.No.A00186) is recommended.