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Lyophilized goat IgG fraction to human complement C3 and buffer salts.

Lyophilized goat IgG fraction to human complement C4 and buffer salts.

Polyclonal Antibody against human IgA alpha chain. Suitable for immunostaining and immunodetection.

Lyophilized goat IgG fraction to human IgM (5Fc μ) and buffer salts.

Goat Serum IgG Fraction to human serum.

Lyophilized goat IgG fraction to mouse complement C3 and buffer salts.

Lyophilized goat IgG fraction to mouse IgG (whole molecule) and buffer salts.

Lyophilized goat IgG fraction to rat complement C3 and buffer salts.

Lyophilized rabbit IgG fraction to hamster IgG (whole molecule) and buffer salts.

Lyophilized rabbit IgG fraction to human albumin and buffer salts.

Lyophilized rabbit polyclonal antiboody againts human red blood cells. For immunoblotting, immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections.

Lyophilized rabbit IgG fraction to mouse IgG (whole molecule) and buffer salts.

Lyophilized rabbit IgG fraction to sheep red blood cells and buffer salts.

Human IgG positive control is lyophilized human IgG positive control with buffers and salts. IgG is a purified antigen.

98% purity by SDS PAGE. No trace of IgA, IgM or non-immunoglobulin serum proteins

Lyophilized monkey IgG with buffers and salts.

Mouse IgG purified from normal mouse serum.

Mouse IgG purified from normal mouse serum.

IgG, rabbit, fluorescein-conjugated

Lyophilized rabbit IgG F(ab’)2 with buffer salts.

Purified rat IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Rat IgG was used as isotype control in flow cytometry and injected into mice to induce tolerance against rat IgG.

IgG/IgM Depleted Human Complement

IgG/IgM Depleted Human Complement

IgG/IgM Depleted Human Complement

IgG/IgM Depleted Human Serum Heat-Inactivated

IgG/IgM Depleted Human Serum Heat-Inactivated

IgG/IgM Depleted Human Serum Heat-Inactivated

IgG/IgM Depleted Human Serum Heat-Inactivated

Mouse IgG1 (K) purified from clarified mouse MOPC 21 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.02 M TRIS, 0.15 M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.

[MOPC 21]
Ascites fluid, collected from Balb/c mice which carry plasmacytomas subcutaneously, are clarified by centrifugation and filtration. It is dialyzed, adjusted to yield at least 5 mg of immunoglobulin, then vialed and lyophilized. Clarified ascites are tested for immunoglobulin by immunoelectrophoresis using antisera specific for the particular type and isotype. Approximately 10% of the total protein is immunoglobulin (including trace levels of the host’s immunoglobulins), 80% is albumin, and 10% is complement and other proteins.

From myeloma source, reactive against IgG1 by subclass-specific radial immunodiffusion and ELISA. This product contains 1% IgG2 , IgG3 , and IgG4 .

From myeloma source, reactive against IgG1 by subclass-specific radial immunodiffusion and ELISA. This product contains 1% IgG2 , IgG3 , and IgG4 .

From myeloma source, this product is reactive specific to its subclass by subclass-specific radial immunodiffusion and ELISA. It contains % IgG1 , IgG3 , and IgG4 .

IgG2A (Kappa) UPC 10, mouse myeloma, purified

Mouse IgG2b is purified from clarified mouse MOPC 141 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography, and immunoabsorption. The product is dialyzed into 0.02 M Tris 0.15M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.

Mouse IgG2b (j) is purified from clarified mouse MOPC 195 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.02 M Tris, 0.15 M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.

Lyophilized Clarified mouse myeloma ascites IgG2b (j) MOPC 195.

Mouse IgG3 (k) is purified from clarified mouse FLOPC 21 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.02 M Tris, 0.15 M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.

[MOPC 104E]
These myeloma proteins are purified from ascites by a combination of salt precipitation, ion exchange and bioaffinity chromatography. Purified proteins are dialyzed into 0.02M Tris, 0.14M sodium chloride, pH 8.1. Product is adjusted to 1.0 mg/ml, filtered, vialed, and stored frozen at -70°C. Product is shipped on dry ice.

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Product No. 31172 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Product No. 31172 reacts with the heavy chain portion of mouse IgM, but does not react against IgG or non-immunoglobulin serum proteins. However, this antibody may cross-react with IgM from other species. Store product at 4°C until opened. After opening, dilute only enough antibody for a single day’s use. Store remainder at 4°C under sterile conditions. Country of Origin: USA

nThermo Scientific Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.