5

Heidolph Radleys Starfish Viton Straps 200mm

Hektoen Enteric (HE) Agar, 5 kg

Hematin

Hematoporphyrin

Hematoxylin

Hematoxylin, Hydrate

HEPES Sodium Salt, Biological Buffer, High Purity

HEPES, Biological Buffer

HEPES, Biological Buffer, Ultrapure

Heptadecafluoro-n-octyl Bromide

Heptadecafluorononanoic Acid

Hexacosane

Hexadecyltrimethylammonium Chloride

Hexaethylene Glycol

Hexaethylene Glycol Monomethyl Ether

Hexafluoroacetylacetone

Hexamethylbenzene

Hexamethylditin

Hexamethylphosphoramide

Hexaphenylbenzene

Hexaphenyldisilane

GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni²⁺ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin FF has low Ni²⁺ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin FF Prepacked Column the excellent choice for high performance purification of polyhistidine-tagged proteins.

Storage
Store in 20% ethanol at 2°C to 8°C. Do not freeze.

Characteristics

Column compatibility

The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).

Reagents Compatible

GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni²⁺ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin FF has low Ni²⁺ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin FF Prepacked Column the excellent choice for high performance purification of polyhistidine-tagged proteins.

Storage
Store in 20% ethanol at 2°C to 8°C. Do not freeze.

Characteristics

Column compatibility

The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).

Reagents Compatible

Description

His Tag Antibody Plate is a microtiter plate coated with THE^TM Anti His Tag Antibody. The plate is designed for capturing His tagged protein with His tag located at N-terminus, C-terminus, or internal of the protein. It can be applied for analysis of different length of His tag including Hexa-His (6 His), Penta-His (5-His) and Tetra-His (4-His).The plate demonstrate high sensitivity and high binding capacity. The capacity and sensitivity varies, depending on protein size, structure and solution buffer. Generally, proteins with lower molecular weight (M.W) can be more sensitive to the His antibody plate than ones with higher M.W. Compared with Nickel Coated Plate, the affinity plate can bind target proteins with high specificity, and tolerable with several common used reagents such as EDTA, imidazole and β-ME.

Description

His Tag Antibody Plate is a microtiter plate coated with THE^TM Anti His Tag Antibody. The plate is designed for capturing His tagged protein with His tag located at N-terminus, C-terminus, or internal of the protein. It can be applied for analysis of different length of His tag including Hexa-His (6 His), Penta-His (5-His) and Tetra-His (4-His).The plate demonstrate high sensitivity and high binding capacity. The capacity and sensitivity varies, depending on protein size, structure and solution buffer. Generally, proteins with lower molecular weight (M.W) can be more sensitive to the His antibody plate than ones with higher M.W. Compared with Nickel Coated Plate, the affinity plate can bind target proteins with high specificity, and tolerable with several common used reagents such as EDTA, imidazole and β-ME.

Description

His Tag Antibody Plate is a microtiter plate coated with THE^TM Anti His Tag Antibody in a non-removable format. The plate is designed for capturing His tagged protein with His tag located at N-terminus, C-terminus, or internal of the protein. It can be applied for analysis of different length of His tag including Hexa-His (6 His), Penta-His (5-His) and Tetra-His (4-His).The plate demonstrate high sensitivity and high binding capacity. The capacity and sensitivity varies, depending on protein size, structure and solution buffer. Generally, proteins with lower molecular weight (M.W) can be more sensitive to the His antibody plate than ones with higher M.W. Compared with Nickel Coated Plate, the affinity plate can bind target proteins with high specificity, and tolerable with several common used reagents such as EDTA, imidazole and 2-ME.

Histamine Dihydrochloride, Reagent

Histamine Diphosphate, Hydrate

Histamine, Reagent