L00951-1

Showing the single result

• 

  MonoRab™ Anti-Humanized VHH Affinity Resin FF

  Camelid
  VHH Antibody (also called single-domain antibody, sdAb or Nanobody by Ablynx)
  is a peptide chain about 110 amino acids in length, containing one variable
  domain of a heavy-chain antibody (a VHH fragment). Like a whole antibody, the camelid
  VHH antibody is able to bind selectively to a specific antigen. This single-domain
  antibody has a broad range of applications in biotechnical and therapeutic research
  fields due to its small size, simple production and high affinity. However, their sequences may increase the risk of immunogenicity and
  anti-drug antibody (ADA) development in humans, and thus, sdAbs are routinely
  humanized during development.  
  GenScript’s MonoRab™ Anti- Humanized VHH Affinity
  Resin FF (Cat. No. L00951) is designed for purification of humanized VHH
  single domain antibody from commonly used protein expression systems such as bacteria,
  yeast and mammalian cells. The VHH single domain antibody in cell lysate can specifically
  bind to the Anti- Humanized VHH monoclonal antibody coupled to the resin. Nonspecific binding
  reactions are eliminated by stringent washing steps, and the purified target
  protein is eluted with a high recovery rate. Table 1 lists the main characteristics
  of GenScript’s MonoRab™ Anti- Humanized VHH Affinity Resin FF. 

  ---

  Table 1. Characteristics of Anti-Humanized VHH
  Affinity Resin FF

  Product content	50% settled resin in TBS with 0.02% sodium azide
  Matrix	4% highly cross-linked agarose
  Average bead size	90 μm
  Ligand	MonoRab™ Anti-Humanized VHH Antibody
  Binding capacity	> 2 mg Humanized VHH per mL settled resin (depending on amino acid sequence of different Humanized VHHs)
  Storage and stability	Store at 2-8 °C for up to 12 months. Do not freeze the resin.
  Resin reuse	When stored at 2 – 8° C, the resin can be recycled at least 5 times with no loss of binding capability. While, it can be reused up to 10 times with minimum loss of binding capability.
  Elution method	Acid buffer elution (pH 2.5)
  Reagents compatibility	Compatible with commonly used bacterial lysis reagents. For reagents compatible with purifications, see tables 2 and 3 in the manual.

  $409.69 Add to cart View Product Details