GenScript Biotech

GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) features high binding capacity, specificity, sensitivity, stability, and reusability, making it particularly useful for the affinity purification and immunoprecipitation of DYKDDDDK-tagged fusion proteins expressed in common protein expression systems such as bacteria, yeast, and mammalian cells.

GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) features high binding capacity, specificity, sensitivity, stability, and reusability, making it particularly useful for the affinity purification and immunoprecipitation of DYKDDDDK-tagged fusion proteins expressed in common protein expression systems such as bacteria, yeast, and mammalian cells.

GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) features high binding capacity, specificity, sensitivity, stability, and reusability, making it particularly useful for the affinity purification and immunoprecipitation of DYKDDDDK-tagged fusion proteins expressed in common protein expression systems such as bacteria, yeast, and mammalian cells.

GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) features high binding capacity, specificity, sensitivity, stability, and reusability, making it particularly useful for the affinity purification and immunoprecipitation of DYKDDDDK-tagged fusion proteins expressed in common protein expression systems such as bacteria, yeast, and mammalian cells.

This product is designed for purification of proteins, antibodies and compounds containing (GnS)m linker (n≥2, m≥2) from commonly used protein expression systems such as bacteria, yeast and mammalian cells.

This product is designed for purification of proteins, antibodies and compounds containing (GnS)m linker (n≥2, m≥2) from commonly used protein expression systems such as bacteria, yeast and mammalian cells.

This product is designed for purification of proteins, antibodies and compounds containing (GnS)m linker (n≥2, m≥2) from commonly used protein expression systems such as bacteria, yeast and mammalian cells.

This product is designed for purification of proteins, antibodies and compounds containing (GnS)m linker (n≥2, m≥2) from commonly used protein expression systems such as bacteria, yeast and mammalian cells.

Principle
Add the sample containing the HA-tagged protein
to the Anti-HA IP Resin and allow the protein bind to the Resin during a short
time incubation. Then the HA-tagged protein can be eluted off the beads, or
used directly for pull-down of target protein.

Material Supplied
Genscript Anti-HA IP Resin is an agarose beads
coupled to THE™ HA Tag Antibody, mAb, Mouse (Genscript, A01244).
The antibody recognizes C-terminal, N-terminal, and internal HA tagged-fusion
proteins. It is supplied as a suspension in 50% glycerol containing 10 mM sodium phosphate, 150 mM
sodium chloride, pH 7.4, 0.02 % (w/v) sodium azide (PBS/A).

Storage and Stability
Store the resin: Anti-HA IP Resin is supplied at
-20°C.Do not store the gel at freezing temperatures in the absence of glycerol. 
Store the Column: Wash the column three times
with 5 ml of TBS/A (TBS containing 0.02 % sodium azide) then add another 5 ml
of TBS/A and store at 2-8°C without draining.

Additional Buffers Needed
Equilibration buffer:  50 mM Tris, 150 mM NaCl, pH 7.4.
1×SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at
25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue.

Principle
Add the sample containing the HA-tagged protein
to the Anti-HA IP Resin and allow the protein bind to the Resin during a short
time incubation. Then the HA-tagged protein can be eluted off the beads, or
used directly for pull-down of target protein.

Material Supplied
Genscript Anti-HA IP Resin is an agarose beads
coupled to THE™ HA Tag Antibody, mAb, Mouse (Genscript, A01244).
The antibody recognizes C-terminal, N-terminal, and internal HA tagged-fusion
proteins. It is supplied as a suspension in 50% glycerol containing 10 mM sodium phosphate, 150 mM
sodium chloride, pH 7.4, 0.02 % (w/v) sodium azide (PBS/A).

Storage and Stability
Store the resin: Anti-HA IP Resin is supplied at
-20°C.Do not store the gel at freezing temperatures in the absence of glycerol. 
Store the Column: Wash the column three times
with 5 ml of TBS/A (TBS containing 0.02 % sodium azide) then add another 5 ml
of TBS/A and store at 2-8°C without draining.

Additional Buffers Needed
Equilibration buffer:  50 mM Tris, 150 mM NaCl, pH 7.4.
1×SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at
25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue.