GenScript Biotech

GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) features high binding capacity, specificity, sensitivity, stability, and reusability, making it particularly useful for the affinity purification and immunoprecipitation of DYKDDDDK-tagged fusion proteins expressed in common protein expression systems such as bacteria, yeast, and mammalian cells.

GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) features high binding capacity, specificity, sensitivity, stability, and reusability, making it particularly useful for the affinity purification and immunoprecipitation of DYKDDDDK-tagged fusion proteins expressed in common protein expression systems such as bacteria, yeast, and mammalian cells.

GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) features high binding capacity, specificity, sensitivity, stability, and reusability, making it particularly useful for the affinity purification and immunoprecipitation of DYKDDDDK-tagged fusion proteins expressed in common protein expression systems such as bacteria, yeast, and mammalian cells.

GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) features high binding capacity, specificity, sensitivity, stability, and reusability, making it particularly useful for the affinity purification and immunoprecipitation of DYKDDDDK-tagged fusion proteins expressed in common protein expression systems such as bacteria, yeast, and mammalian cells.

GenScript Anti‐DYKDDDDK G1 Affinity Resin (Cat. No. L00432) is designed for the purification of DYKDDDDK‐tagged protein from commonly used protein expression systems including bacteria, yeast and mammalian cells.

GenScript Anti‐DYKDDDDK G1 Affinity Resin (Cat. No. L00432) is designed for the purification of DYKDDDDK‐tagged protein from commonly used protein expression systems including bacteria, yeast and mammalian cells.

GenScript Anti‐DYKDDDDK G1 Affinity Resin (Cat. No. L00432) is designed for the purification of DYKDDDDK‐tagged protein from commonly used protein expression systems including bacteria, yeast and mammalian cells.

GenScript Anti‐DYKDDDDK G1 Affinity Resin (Cat. No. L00432) is designed for the purification of DYKDDDDK‐tagged protein from commonly used protein expression systems including bacteria, yeast and mammalian cells.

GenScript Anti-DYKDDDDK IP Resin is designed for quick and efficient immunoprecipitation of DYKDDDDK-tagged protein or DYKDDDDK-tagged protein-target protein complex. The resin is an agarose beads coupled to Mouse anti-DYKDDDDK monoclonal antibody (clone 6H8E4). The antibody recognizes N-terminal, internal and C-terminal fusion protein containing DYKDDDDK tag. It is supplied as a suspension in 50 % glycerol containing 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.4,0.02 % (w/v) sodium azide (PBS/A).

This product is designed for purification of proteins, antibodies and compounds containing (GnS)m linker (n≥2, m≥2) from commonly used protein expression systems such as bacteria, yeast and mammalian cells.

This product is designed for purification of proteins, antibodies and compounds containing (GnS)m linker (n≥2, m≥2) from commonly used protein expression systems such as bacteria, yeast and mammalian cells.

This product is designed for purification of proteins, antibodies and compounds containing (GnS)m linker (n≥2, m≥2) from commonly used protein expression systems such as bacteria, yeast and mammalian cells.

This product is designed for purification of proteins, antibodies and compounds containing (GnS)m linker (n≥2, m≥2) from commonly used protein expression systems such as bacteria, yeast and mammalian cells.

Principle
Add the sample containing the HA-tagged protein
to the Anti-HA IP Resin and allow the protein bind to the Resin during a short
time incubation. Then the HA-tagged protein can be eluted off the beads, or
used directly for pull-down of target protein.

Material Supplied
Genscript Anti-HA IP Resin is an agarose beads
coupled to THE™ HA Tag Antibody, mAb, Mouse (Genscript, A01244).
The antibody recognizes C-terminal, N-terminal, and internal HA tagged-fusion
proteins. It is supplied as a suspension in 50% glycerol containing 10 mM sodium phosphate, 150 mM
sodium chloride, pH 7.4, 0.02 % (w/v) sodium azide (PBS/A).

Storage and Stability
Store the resin: Anti-HA IP Resin is supplied at
-20°C.Do not store the gel at freezing temperatures in the absence of glycerol. 
Store the Column: Wash the column three times
with 5 ml of TBS/A (TBS containing 0.02 % sodium azide) then add another 5 ml
of TBS/A and store at 2-8°C without draining.

Additional Buffers Needed
Equilibration buffer:  50 mM Tris, 150 mM NaCl, pH 7.4.
1×SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at
25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue.

Principle
Add the sample containing the HA-tagged protein
to the Anti-HA IP Resin and allow the protein bind to the Resin during a short
time incubation. Then the HA-tagged protein can be eluted off the beads, or
used directly for pull-down of target protein.

Material Supplied
Genscript Anti-HA IP Resin is an agarose beads
coupled to THE™ HA Tag Antibody, mAb, Mouse (Genscript, A01244).
The antibody recognizes C-terminal, N-terminal, and internal HA tagged-fusion
proteins. It is supplied as a suspension in 50% glycerol containing 10 mM sodium phosphate, 150 mM
sodium chloride, pH 7.4, 0.02 % (w/v) sodium azide (PBS/A).

Storage and Stability
Store the resin: Anti-HA IP Resin is supplied at
-20°C.Do not store the gel at freezing temperatures in the absence of glycerol. 
Store the Column: Wash the column three times
with 5 ml of TBS/A (TBS containing 0.02 % sodium azide) then add another 5 ml
of TBS/A and store at 2-8°C without draining.

Additional Buffers Needed
Equilibration buffer:  50 mM Tris, 150 mM NaCl, pH 7.4.
1×SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at
25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue.

GenScript Anti-His Affinity Resin is designed for purification of His-tagged protein. It is composed of agarose and conjugated THE^TM anti-His monoclonal antibody. His-tagged protein in the crude samples can bind to the anti-His monoclonal antibodies coupled with the agarose, and the nonspecific proteins can be eliminated by washing. As a result, the His-tagged protein will finally be eluted with high recovery. Moreover, this product can be used for immunoprecipitation of His-tagged protein.

GenScript Anti-His Affinity Resin is designed for purification of His-tagged protein. It is composed of agarose and conjugated THE^TM anti-His monoclonal antibody. His-tagged protein in the crude samples can bind to the anti-His monoclonal antibodies coupled with the agarose, and the nonspecific proteins can be eliminated by washing. As a result, the His-tagged protein will finally be eluted with high recovery. Moreover, this product can be used for immunoprecipitation of His-tagged protein.