Thermo Scientific

Thermo Scientific™ Pierce Triethylamine (TEA) is a high-purity solvent that can be used in certain HPLC protocols, such as weak anion exchange to resolve certain tryptic peptides on a reverse-phase column.

nTriethylamine is an ion-pairing reagent that alters selectivity in reverse-phase HPLC separations. By pairing with peptides, it effectively sharpens peaks, resulting in improved peak resolution.

Thermo Scientific™ Pierce Western Blot Signal Enhancer is a two-reagent system for conditioning protein blots after transfer to greatly enhance the effectiveness of primary antibodies and intensify the final detection signal in Western blot experiments.

The Pierce Western Blot Signal Enhancer membrane treatment procedure is very simple, takes only 15 minutes and can be added to nearly any existing Western blotting protocol. The result is an increase in the intensity of target protein bands on the Western blot or detection of target proteins at levels that were previously not possible. The product is effective for signal intensification with both chemiluminescent and chromogenic substrates, especially with nitrocellulose membranes.

Highlights:

• Increases protein detection – most protein targets show a three- to 10-fold increase in signal intensity, enabling much less protein to be detected with the same substrate and method
• Improves antibody binding – the membrane-treatment reagent exposes and conditions target proteins so that specific antibodies can bind more effectively
• Works for nearly any protein – signal enhancement has been demonstrated with targets such as IL-6, p53, NFkB, BRCA1 and EGF
• Effective with any substrate – enhances both chemiluminescent (ECL) and colorimetric detection for Western blots
• Compatible with any membrane – enhances signal on nitrocellulose and PVDF membrane, regardless of pore size (enhancement is less pronounced with PVDF)
• Fast, 15-minute protocol – optimized for a combination of simplicity, speed and signal enhancement for most proteins
• Ready-to-use – no formulating or diluting necessary, and the reagents are stable for storage at room temperature

Includes:

Enhancer Reagent 1, 250mL; Enhancer Reagent 2, 250mL

Help reduce variability in molecular orientation with Thermo Scientific™ Plates and Modules with Affinity Binding Surfaces.

Affinity Capture Surfaces (Passive Streptavidin and Immobilizer Streptavidin, Nickel Chelate and Glutathione, BioBind)

• • Highly specific binding

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• • Reduced variability in molecular orientation

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• • Immobilizer surfaces improve signal to noise ratios

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• • Streptavidin biotin interaction can be exploited to immobilize a wide range of biomolecules

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Help reduce variability in molecular orientation with Thermo Scientific™ Plates and Modules with Affinity Binding Surfaces.

Affinity Capture Surfaces (Passive Streptavidin and Immobilizer Streptavidin, Nickel Chelate and Glutathione, BioBind)

• • Highly specific binding

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• • Reduced variability in molecular orientation

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• • Immobilizer surfaces improve signal to noise ratios

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• • Streptavidin biotin interaction can be exploited to immobilize a wide range of biomolecules

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Help reduce variability in molecular orientation with Thermo Scientific™ Plates and Modules with Affinity Binding Surfaces.

Affinity Capture Surfaces (Passive Streptavidin and Immobilizer Streptavidin, Nickel Chelate and Glutathione, BioBind)

• • Highly specific binding

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• • Reduced variability in molecular orientation

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• • Immobilizer surfaces improve signal to noise ratios

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• • Streptavidin biotin interaction can be exploited to immobilize a wide range of biomolecules

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Reduce the amount of reagents used in the lab with Thermo Scientific™ Plates and Modules with Covalent Binding Surfaces.      Covalent Surfaces (Thermo Scientific™ CovaLink™ and Immobilizer Amino)

• • Minimal leaching

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• • Withstands vigorous washing

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• • Coating with lower amounts of reagent may be possible

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• • Control of orientation

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Reduce the amount of reagents used in the lab with Thermo Scientific™ Plates and Modules with Covalent Binding Surfaces.

Covalent Surfaces (Thermo Scientific™ CovaLink™ and Immobilizer Amino)

• • Minimal leaching

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• • Withstands vigorous washing

n

• • Coating with lower amounts of reagent may be possible

n

• • Control of orientation

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Reduce the amount of reagents used in the lab with Thermo Scientific™ Plates and Modules with Covalent Binding Surfaces.

Covalent Surfaces (Thermo Scientific™ CovaLink™ and Immobilizer Amino)

• • Minimal leaching

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• • Withstands vigorous washing

n

• • Coating with lower amounts of reagent may be possible

n

• • Control of orientation

n

Reduce the amount of reagents used in the lab with Thermo Scientific™ Plates and Modules with Covalent Binding Surfaces.

Covalent Surfaces (Thermo Scientific™ CovaLink™ and Immobilizer Amino)

• • Minimal leaching

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• • Withstands vigorous washing

n

• • Coating with lower amounts of reagent may be possible

n

• • Control of orientation

n

Reduce the amount of reagents used in the lab with Thermo Scientific™ Plates and Modules with Covalent Binding Surfaces.nn nnCovalent Surfaces (Thermo Scientific™ CovaLink™ and Immobilizer Amino)n

• • Minimal leaching

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• • Withstands vigorous washing

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• • Coating with lower amounts of reagent may be possible

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• • Control of orientation

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Immobilizer is a trademark of Exiqon A/S, Vedbaek, Denmark. The product is produced under license from Exiqon A/S and covered by EP 08 20483 and foreign application and patents.

Reduce the amount of reagents used in the lab with Thermo Scientific™ Plates and Modules with Covalent Binding Surfaces.

Covalent Surfaces (Thermo Scientific™ CovaLink™ and Immobilizer Amino)

• Minimal leaching
• Withstands vigorous washing
• Coating with lower amounts of reagent may be possible
• Control of orientation

Immobilizer is a trademark of Exiqon A/S, Vedbaek, Denmark. The product is produced under license from Exiqon A/S and covered by EP 08 20483 and foreign application and patents.

Rapid Equilibrium Dialysis (RED) Devices have been widely adopted as an effective in vitro technique for conducting plasma protein binding (PPB) assays in drug development. This measurement is used to assess the biological availability of drug candidates and guides critical downstream decisions such as drug design, in vivo testing prioritization, and drug-drug interaction evaluation. RED Devices were developed through close collaboration with the pharmaceutical industry, ensuring that they meet the highest standards of accuracy and reliability in equilibrium dialysis testing.

Equilibrium dialysis is commonly used in drug-plasma protein binding (PPB) studies to quantitate the free fraction of drugs in plasma. The binding of drugs to plasma proteins is a critical factor that affects their pharmacokinetics and pharmacodynamics, as only the free fraction of a drug can exert a physiological effect. The Thermo Scientific Pierce Rapid Equilibrium Dialysis (RED) Device is a superior method for conducting this analysis because it delivers accurate and consistent data with shorter preparation and dialysis times.

RED Devices use a size-defined dialysis membrane to quantitate the amount of free (active) drug molecules that are not bound to plasma proteins. They consist of disposable inserts and a base plate with a standard microplate footprint. The inserts are made of two side-by-side chambers separated by a dialysis membrane and are used along with a required base plate or are available as single-use plates with Inserts. Competition RED Devices are also available to enable competitive dialysis experiments with multiple tissue or protein fluid samples.

Features of RED Devices include:
• Ease of use—disposable tubes do not require presoaking, assembly, or specialized equipment
• Rapid dialysis—high surface-to-volume ratio of the membrane enables equilibrium in 2–4 hours
• High-throughput—96-well footprint is suitable for automation
• Multi-sample processing—can be used for 1–48 assays/plate without wasting an entire plate
• Robustness—compartmentalized design eliminates the potential for cross-contamination or leakage
• Reproducibility and accuracy—validated for plasma protein binding assays, producing results consistent with those reported in the literature
• Flexible format—available in pre-inserted disposable polypropylene plates and packs of individual inserts

In addition to plasma protein binding, the device is used for determining drug partitioning between red blood cells and plasma, protein binding of liver microsomes to improve the correlation between in vitro and in vivo intrinsic clearance, drug metabolism and pharmacokinetics (DMPK) assays, and competition between tissue protein binding against plasma proteins.

RED Device Inserts, 8K MWCO
Pierce RED Device Inserts are designed for use with a required base plate for equilibrium dialysis experiments. Each single-use, disposable insert is made of two side-by-side chambers separated by an O-ring-sealed vertical cylinder of dialysis membrane (8K MWCO) validated for minimal nonspecific binding. They are available in packs of 50 or 250 each and are useful for testing a variable number of samples per run.

RED Device Base Plates
Pierce RED Device Inserts are used with either the RED Device Reusable Base Plate made of high-grade PTFE (Teflon™) or the RED Device Single-Use Base Plate made of high-density polypropylene. The Single-Use Base Plate is disposable and lightweight, allowing routine automation. Each Base Plate holds up to 48 RED Device Inserts and has a standard 96-well plate footprint with 9 x 9 mm well spacing for compatibility with multichannel pipettors and automated liquid handling systems.

RED Device Single-Use Plate with Inserts, 8K or 12K MWCO
The RED Device Single-Use Plate with Inserts is composed of disposable high-density polypropylene and comes preloaded with 48 equilibrium dialysis membrane inserts. Each insert includes two side-by-side chambers separated by an O ring sealed vertical cylinder of dialysis membrane (8K or 12K MWCO). The device is automation-friendly and has a standard 96-well plate footprint with 9 x 9 mm well spacing. The single-use plate is easily disposed of to avoid contamination and cleaning, making it useful when performing PPB assays with radioactive or hazardous materials.

Competition RED Device Inserts and Base Plate, 12K MWCO
The Pierce Competition RED Device facilitates the analysis of simultaneous drug interactions and partitioning among multiple tissues to accurately model in vivo drug interactions. The system consists of disposable dialysis tube inserts (12K MWCO) and a reusable base plate made of chemically inert high-grade PTFE, eliminating non-specific binding and risk of contamination. The base plate is divided into different size chambers for positioning 2–8 RED Device Inserts per well, enabling competitive dialysis experiments with 2–15 separate tissue or protein fluid samples. Each Competition RED Insert contains either one or two separate dialysis chambers (each package includes both types).

Applications of the Competition RED Devices include:
• ADME-Tox studies: in vitro screening of drug partitioning between plasma and multiple tissues before in vivo studies with animal models
• Determine formulation of drug dosage for in vivo studies
• Drug-drug interaction studies
• Competitive binding and dissociation constant determination for small molecules versus multiple targets

RED Device Insert Removal Tool
This tool enables fast removal of 8 inserts at once.

Rapid Equilibrium Dialysis (RED) Devices have been widely adopted as an effective in vitro technique for conducting plasma protein binding (PPB) assays in drug development. This measurement is used to assess the biological availability of drug candidates and guides critical downstream decisions such as drug design, in vivo testing prioritization, and drug-drug interaction evaluation. RED Devices were developed through close collaboration with the pharmaceutical industry, ensuring that they meet the highest standards of accuracy and reliability in equilibrium dialysis testing.

Equilibrium dialysis is commonly used in drug-plasma protein binding (PPB) studies to quantitate the free fraction of drugs in plasma. The binding of drugs to plasma proteins is a critical factor that affects their pharmacokinetics and pharmacodynamics, as only the free fraction of a drug can exert a physiological effect. The Thermo Scientific Pierce Rapid Equilibrium Dialysis (RED) Device is a superior method for conducting this analysis because it delivers accurate and consistent data with shorter preparation and dialysis times.

RED Devices use a size-defined dialysis membrane to quantitate the amount of free (active) drug molecules that are not bound to plasma proteins. They consist of disposable inserts and a base plate with a standard microplate footprint. The inserts are made of two side-by-side chambers separated by a dialysis membrane and are used along with a required base plate or are available as single-use plates with Inserts. Competition RED Devices are also available to enable competitive dialysis experiments with multiple tissue or protein fluid samples.

Features of RED Devices include:
• Ease of use—disposable tubes do not require presoaking, assembly, or specialized equipment
• Rapid dialysis—high surface-to-volume ratio of the membrane enables equilibrium in 2–4 hours
• High-throughput—96-well footprint is suitable for automation
• Multi-sample processing—can be used for 1–48 assays/plate without wasting an entire plate
• Robustness—compartmentalized design eliminates the potential for cross-contamination or leakage
• Reproducibility and accuracy—validated for plasma protein binding assays, producing results consistent with those reported in the literature
• Flexible format—available in pre-inserted disposable polypropylene plates and packs of individual inserts

In addition to plasma protein binding, the device is used for determining drug partitioning between red blood cells and plasma, protein binding of liver microsomes to improve the correlation between in vitro and in vivo intrinsic clearance, drug metabolism and pharmacokinetics (DMPK) assays, and competition between tissue protein binding against plasma proteins.

RED Device Inserts, 8K MWCO
Pierce RED Device Inserts are designed for use with a required base plate for equilibrium dialysis experiments. Each single-use, disposable insert is made of two side-by-side chambers separated by an O-ring-sealed vertical cylinder of dialysis membrane (8K MWCO) validated for minimal nonspecific binding. They are available in packs of 50 or 250 each and are useful for testing a variable number of samples per run.

RED Device Base Plates
Pierce RED Device Inserts are used with either the RED Device Reusable Base Plate made of high-grade PTFE (Teflon™) or the RED Device Single-Use Base Plate made of high-density polypropylene. The Single-Use Base Plate is disposable and lightweight, allowing routine automation. Each Base Plate holds up to 48 RED Device Inserts and has a standard 96-well plate footprint with 9 x 9 mm well spacing for compatibility with multichannel pipettors and automated liquid handling systems.

RED Device Single-Use Plate with Inserts, 8K or 12K MWCO
The RED Device Single-Use Plate with Inserts is composed of disposable high-density polypropylene and comes preloaded with 48 equilibrium dialysis membrane inserts. Each insert includes two side-by-side chambers separated by an O ring sealed vertical cylinder of dialysis membrane (8K or 12K MWCO). The device is automation-friendly and has a standard 96-well plate footprint with 9 x 9 mm well spacing. The single-use plate is easily disposed of to avoid contamination and cleaning, making it useful when performing PPB assays with radioactive or hazardous materials.

Competition RED Device Inserts and Base Plate, 12K MWCO
The Pierce Competition RED Device facilitates the analysis of simultaneous drug interactions and partitioning among multiple tissues to accurately model in vivo drug interactions. The system consists of disposable dialysis tube inserts (12K MWCO) and a reusable base plate made of chemically inert high-grade PTFE, eliminating non-specific binding and risk of contamination. The base plate is divided into different size chambers for positioning 2–8 RED Device Inserts per well, enabling competitive dialysis experiments with 2–15 separate tissue or protein fluid samples. Each Competition RED Insert contains either one or two separate dialysis chambers (each package includes both types).

Applications of the Competition RED Devices include:
• ADME-Tox studies: in vitro screening of drug partitioning between plasma and multiple tissues before in vivo studies with animal models
• Determine formulation of drug dosage for in vivo studies
• Drug-drug interaction studies
• Competitive binding and dissociation constant determination for small molecules versus multiple targets

Rapid Equilibrium Dialysis (RED) Devices have been widely adopted as an effective in vitro technique for conducting plasma protein binding (PPB) assays in drug development. This measurement is used to assess the biological availability of drug candidates and guides critical downstream decisions such as drug design, in vivo testing prioritization, and drug-drug interaction evaluation. RED Devices were developed through close collaboration with the pharmaceutical industry, ensuring that they meet the highest standards of accuracy and reliability in equilibrium dialysis testing.

Equilibrium dialysis is commonly used in drug-plasma protein binding (PPB) studies to quantitate the free fraction of drugs in plasma. The binding of drugs to plasma proteins is a critical factor that affects their pharmacokinetics and pharmacodynamics, as only the free fraction of a drug can exert a physiological effect. The Thermo Scientific Pierce Rapid Equilibrium Dialysis (RED) Device is a superior method for conducting this analysis because it delivers accurate and consistent data with shorter preparation and dialysis times.

RED Devices use a size-defined dialysis membrane to quantitate the amount of free (active) drug molecules that are not bound to plasma proteins. They consist of disposable inserts and a base plate with a standard microplate footprint. The inserts are made of two side-by-side chambers separated by a dialysis membrane and are used along with a required base plate or are available as single-use plates with Inserts. Competition RED Devices are also available to enable competitive dialysis experiments with multiple tissue or protein fluid samples.

Features of RED Devices include:
• Ease of use—disposable tubes do not require presoaking, assembly, or specialized equipment
• Rapid dialysis—high surface-to-volume ratio of the membrane enables equilibrium in 2–4 hours
• High-throughput—96-well footprint is suitable for automation
• Multi-sample processing—can be used for 1–48 assays/plate without wasting an entire plate
• Robustness—compartmentalized design eliminates the potential for cross-contamination or leakage
• Reproducibility and accuracy—validated for plasma protein binding assays, producing results consistent with those reported in the literature
• Flexible format—available in pre-inserted disposable polypropylene plates and packs of individual inserts

In addition to plasma protein binding, the device is used for determining drug partitioning between red blood cells and plasma, protein binding of liver microsomes to improve the correlation between in vitro and in vivo intrinsic clearance, drug metabolism and pharmacokinetics (DMPK) assays, and competition between tissue protein binding against plasma proteins.

RED Device Inserts, 8K MWCO
Pierce RED Device Inserts are designed for use with a required base plate for equilibrium dialysis experiments. Each single-use, disposable insert is made of two side-by-side chambers separated by an O-ring-sealed vertical cylinder of dialysis membrane (8K MWCO) validated for minimal nonspecific binding. They are available in packs of 50 or 250 each and are useful for testing a variable number of samples per run.

RED Device Base Plates
Pierce RED Device Inserts are used with either the RED Device Reusable Base Plate made of high-grade PTFE (Teflon™) or the RED Device Single-Use Base Plate made of high-density polypropylene. The Single-Use Base Plate is disposable and lightweight, allowing routine automation. Each Base Plate holds up to 48 RED Device Inserts and has a standard 96-well plate footprint with 9 x 9 mm well spacing for compatibility with multichannel pipettors and automated liquid handling systems.

RED Device Single-Use Plate with Inserts, 8K or 12K MWCO
The RED Device Single-Use Plate with Inserts is composed of disposable high-density polypropylene and comes preloaded with 48 equilibrium dialysis membrane inserts. Each insert includes two side-by-side chambers separated by an O ring sealed vertical cylinder of dialysis membrane (8K or 12K MWCO). The device is automation-friendly and has a standard 96-well plate footprint with 9 x 9 mm well spacing. The single-use plate is easily disposed of to avoid contamination and cleaning, making it useful when performing PPB assays with radioactive or hazardous materials.

Competition RED Device Inserts and Base Plate, 12K MWCO
The Pierce Competition RED Device facilitates the analysis of simultaneous drug interactions and partitioning among multiple tissues to accurately model in vivo drug interactions. The system consists of disposable dialysis tube inserts (12K MWCO) and a reusable base plate made of chemically inert high-grade PTFE, eliminating non-specific binding and risk of contamination. The base plate is divided into different size chambers for positioning 2–8 RED Device Inserts per well, enabling competitive dialysis experiments with 2–15 separate tissue or protein fluid samples. Each Competition RED Insert contains either one or two separate dialysis chambers (each package includes both types).

Applications of the Competition RED Devices include:
• ADME-Tox studies: in vitro screening of drug partitioning between plasma and multiple tissues before in vivo studies with animal models
• Determine formulation of drug dosage for in vivo studies
• Drug-drug interaction studies
• Competitive binding and dissociation constant determination for small molecules versus multiple targets

RED Device Insert Removal Tool
This tool enables fast removal of 8 inserts at once.

Help ensure measurable Class 10 air quality performance with Thermo Scientific™ Replacement HEPA Filters and Cleaning Kits.

HEPA Filters

• • 99.97% filtering at 0.3µm

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• • Compatible with all Thermo Scientific™ Forma™ Series II 3110, Thermo Scientific™ Steri-Cycle™, and Forma™ 310 Series (with HEPA filtration system option) incubators

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• • Main chamber filter available as a stand-alone or packaged in a group with the other chamber in-line filters

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Decontamination Kits

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  • Consists of filters for difficult-to-clean chambers and gaskets (except door) and tubing

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• • Reduces downtime and labor spent on decontamination

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Carry out longer, fatigue-free pipetting with the Thermo Scientific™ S1 Pipette Fillers. These instruments are ergonomically designed and offer simple, efficient pipetting performance, as well as maximum pipetting comfort. Lightweight and cordless, they feature a heavy-duty rechargeable lithium-ion battery that allows approximately three times longer operation between charges than typical units. For use with graduated and volumetric glass and plastic serological pipets. Monitor Performance

• • Large, backlit LCD display provides visual confirmation of remaining battery charge and speed settings

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nControl Speedn

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  • Separate aspirate and dispense speed controls provide precise control over pipetting speeds and reduce risk of overpipetting with smaller pipets

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• • Choose from eight speeds with a simple “+” or “-” to adjust pipetting speed which is displayed on the LCD

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nReady When You Need Itn

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  • Rechargeable lithium-ion battery offers long runtime per charge while eliminating battery memory problems

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• • Battery has rapid charge rate of 80% in one hour and delivers up to 15 hours of continuous operation before recharge

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Warranty and Services

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2-years with web registration

Carry out longer, fatigue-free pipetting with the Thermo Scientific™ S1 Pipette Fillers. These instruments are ergonomically designed and offer simple, efficient pipetting performance, as well as maximum pipetting comfort. Lightweight and cordless, they feature a heavy-duty rechargeable lithium-ion battery that allows approximately three times longer operation between charges than typical units. For use with graduated and volumetric glass and plastic serological pipets.

Slide-A-Lyzer Dialysis Cassettes (20K MWCO) facilitate the simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules larger than 20,000 Daltons. They are available in four sizes to accommodate sample volumes between 0.5 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing faster buffer exchange, and more complete sample recovery.

nSlide-A-Lyzer Dialysis Cassettes (20K MWCO) facilitate the simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules larger than 20,000 Daltons. They are available in four sizes to accommodate sample volumes between 0.5 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing faster buffer exchange and more complete sample recovery. Slide-A-Lyzer cassettes can be used for a wide range of applications including low-molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips, or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

Features of Slide-A-Lyzer Dialysis Cassettes:
• Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
• Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
• High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
• Four cassette sizes—select the cassette that best suits your sample volume
• Color-coded—purple cassette frame specifies cassettes with the 20,000 MW pore size membrane

Membrane specifications:
• Composition: regenerated cellulose synthesized by the Viscose method
• Thickness: 0.9 to 1.2 mil (22.5 to 30 µm)
• Hydration time required before use: 2 minutes
• Glycerol content: none
• Sulfur content: 0.04%
• Heavy metals content: trace

Volume ranges:
• 0.5 mL = 0.1 to 0.5 mL
• 3 mL = 0.5 to 3 mL
• 12 mL = 3 to 12 mL
• 30 mL = 12 to 30 mL

Thermo Scientific™ Pierce Stop Solution for TMB is 0.16M sulfuric acid for use with the ELISA substrate 3,3«,5,5« – tetramethylbenzidine (TMB).

nTMB Stop Solution is 0.16M sulfuric acid for use with the ELISA substrate 3,3«,5,5«-tetramethylbenzidine (TMB). Addition of sulfuric acid stop solution changes the color from blue to yellow, stabilizing the color development to enable accurate measurement of the intensity at 450nm using a spectrophotometer or plate reader.

• • Size: 55mL

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• • Sufficient For: five 96-well microplates

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• • Application: ELISA

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Instructions: Following TMB incubation, add an amount of stop solution equal to the amount of TMB substrate added to each well of the ELISA plate (typically 50 to 100µL per well). Use at full strength.

Thermo Scientific™ Pierce Sulfo-EMCS is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length spacer arm (9.4 Ås).

Highlights:

• Water-soluble
• Cleavable at pH 8.5 using hydroxylamine and heat
• Lactose dehydrogenase retained 60% of its activity after reversible crosslinking
• Reactive groups: sulfo-NHS esters (homobifunctional)
• Reactive toward: amino groups

Thermo Scientific™ Pierce Sulfo-SMPB is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length aromatic spacer arm (11.6 Ås).

Highlights:

• Extended chain length limits steric hindrance
• Water-soluble; membrane-impermeable
• Reactive groups: sulfo-NHS ester and maleimide
• Reactive toward: amino and sulfhydryl groups

Thermo Scientific™ SuperBlock Blocking Buffers are optimized PBS and TBS solutions containing a protein that provides incredibly fast and effective blocking for ELISA, immunohistochemistry and Western blotting applications.

nBlocking microplates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.nnHighlights:n

• • Fast – block membranes in 5 to 10 minutes and ELISA plates in 2 minutes

• • Flexible – guaranteed to be biotin-free for use with streptavidin system

• • Convenience – available in multiple buffer formats (in PBS or TBS with or without Tween™-20 detergent) and package sizes (1L, 5L and dry-blend pouches)

• • Low background – the non-serum protein solution yields a high signal-to-noise ratio

• • Stable – store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Description

Thermo Scientific™ SuperSignal West Femto Substrate is an ultra-sensitive enhanced chemiluminescent substrate (ECL substrate) for detection of peroxidase activity from HRP-conjugated antibodies and probes in Western blotting.

nSuperSignal West Femto Substrate Maximum Sensitivity Substrate is an ultra-sensitive chemiluminescent substrate system for Western blotting with horseradish peroxidase (HRP) enzyme. The substrate kit enables detection of low femtogram (that’s mid-zeptomole!) amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto Substrate enables detection of target proteins in amounts that are small to be seen with typical ECL substrates.nnHighlights:n

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  • ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes

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• • Sensitive – detect low-femtogram (mid-zeptomole) amounts of protein in bands on nitrocellulose or PVDF membranes

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• • Quantitative – produces quantitative signal that is measurable over two orders of magnitude

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• • Saves on antibody – optimized for very dilute antibody concentrations; dilute primary antibodies 5000- to 100,000-fold and secondary antibodies 100,000 to 500,000-fold (from 1mg/mL stocks)

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• • Intense signal – easy to capture an image by exposure to autoradiography film or via a CCD camera or similar imaging system

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• • Excellent signal duration – 8 hours of usable light-output when conditions are optimized

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• • Stable reagent – kit components are stable for 1 year at 4°C or 6 months at room temperature

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Thermo Scientific™ SuperSignal West Femto Substrate is an ultra-sensitive enhanced chemiluminescent substrate (ECL substrate) for detection of peroxidase activity from HRP-conjugated antibodies and probes in Western blotting.

nSuperSignal West Femto Substrate Maximum Sensitivity Substrate is an ultra-sensitive chemiluminescent substrate system for Western blotting with horseradish peroxidase (HRP) enzyme. The substrate kit enables detection of low femtogram (that’s mid-zeptomole!) amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto Substrate enables detection of target proteins in amounts that are small to be seen with typical ECL substrates.nnHighlights:n

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  • ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes

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• • Sensitive – detect low-femtogram (mid-zeptomole) amounts of protein in bands on nitrocellulose or PVDF membranes

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• • Quantitative – produces quantitative signal that is measurable over two orders of magnitude

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• • Saves on antibody – optimized for very dilute antibody concentrations; dilute primary antibodies 5000- to 100,000-fold and secondary antibodies 100,000 to 500,000-fold (from 1mg/mL stocks)

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• • Intense signal – easy to capture an image by exposure to autoradiography film or via a CCD camera or similar imaging system

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• • Excellent signal duration – 8 hours of usable light-output when conditions are optimized

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• • Stable reagent – kit components are stable for 1 year at 4°C or 6 months at room temperature

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• • Ready to use, no preparation required

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• • Validated for use with Thermo Scientific Pierce Matched Antibody Pairs and MiniKits

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• • Size: 100 mL (sufficient for about ten 96-well microplates)

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6X TriTrack DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains three different dyes (bromophenol blue, xylene cyanol FF or orange G) for visual tracking of DNA migration during electrophoresis. The presence of glycerol ensures that the DNA in the ladder and sample forms a layer at the bottom of the well. The EDTA included in the solutions binds divalent metal ions and inhibits metal-dependent nucleases.nn6X TriTrack DNA Loading Dye is used for conventional DNA electrophoresis.

Highlights.

• • Three-color tracking of DNA migration during DNA electrophoresis

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• • No DNA masking during gel exposure to UV light

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• • EDTA binds divalent metal ions and inhibits metal dependent nucleases

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Conduct quantitative and qualitative immunoassays, like ELISA and binding assays, with Thermo Scientific™ White 96-Well Immuno Plates. These plates, which are compatible with common instruments, deliver optimal results, lot-to-lot reliability and well-to-well reproducibility. Features

• • Plate has SBS footprint — fits standard equipment

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• • Available with flat- or round-bottom wells

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• • Choice of three surface types for optimal binding

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• • Certified binding homogeneity/reproducibility

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nChoice of Surface:n

• n
  • Thermo Scientific PolySorp, Microfluor 1, Microlite 1+ and Universal Binding (UB) for adsorption of hydrophobic molecules

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• • Thermo Scientific MaxiSorp is hydrophilic and ideal for antibody sandwich assays

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• • Thermo Scientific Microfluor 2 and Microlite 2+ are slightly hydrophilic and bind a diverse range of biomolecules

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nWhite:n

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  • Gives maximum reflection

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• • Minimum autofluorescence and autoluminescence

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nCompatible with:nnLids for Thermo Scientific MicroWell plates, sealing tape and breathable membrane

Conduct quantitative and qualitative immunoassays, like ELISA and binding assays, with Thermo Scientific™ White 96-Well Immuno Plates. These plates, which are compatible with common instruments, deliver optimal results, lot-to-lot reliability and well-to-well reproducibility.Features

• • Plate has SBS footprint — fits standard equipment

n

• • Available with flat- or round-bottom wells

n

• • Choice of three surface types for optimal binding

n

• • Certified binding homogeneity/reproducibility

n

nChoice of Surface:n

• n
  • Thermo Scientific PolySorp, Microfluor 1, Microlite 1+ and Universal Binding (UB) for adsorption of hydrophobic molecules

n

• • Thermo Scientific MaxiSorp is hydrophilic and ideal for antibody sandwich assays

n

• • Thermo Scientific Microfluor 2 and Microlite 2+ are slightly hydrophilic and bind a diverse range of biomolecules

n

nWhite:n

• n
  • Gives maximum reflection

n

• • Minimum autofluorescence and autoluminescence

n

nCompatible with:nnLids for Thermo Scientific MicroWell plates, sealing tape and breathable membrane

Conduct quantitative and qualitative immunoassays, like ELISA and binding assays, with Thermo Scientific™ White 96-Well Immuno Plates. These plates, which are compatible with common instruments, deliver optimal results, lot-to-lot reliability and well-to-well reproducibility. Features

• • Plate has SBS footprint — fits standard equipment

n

• • Available with flat- or round-bottom wells

n

• • Choice of three surface types for optimal binding

n

• • Certified binding homogeneity/reproducibility

n

nChoice of Surface:n

• n
  • Thermo Scientific PolySorp, Microfluor 1, Microlite 1+ and Universal Binding (UB) for adsorption of hydrophobic molecules

n

• • Thermo Scientific MaxiSorp is hydrophilic and ideal for antibody sandwich assays

n

• • Thermo Scientific Microfluor 2 and Microlite 2+ are slightly hydrophilic and bind a diverse range of biomolecules

n

nWhite:n

• n
  • Gives maximum reflection

n

• • Minimum autofluorescence and autoluminescence

n

nCompatible with:nnLids for Thermo Scientific MicroWell plates, sealing tape and breathable membrane

Conduct quantitative and qualitative immunoassays, like ELISA and binding assays, with Thermo Scientific™ White 96-Well Immuno Plates. These plates, which are compatible with common instruments, deliver optimal results, lot-to-lot reliability and well-to-well reproducibility. Features

• • Plate has SBS footprint — fits standard equipment

n

• • Available with flat- or round-bottom wells

n

• • Choice of three surface types for optimal binding

n

• • Certified binding homogeneity/reproducibility

n

nChoice of Surface:n

• n
  • Thermo Scientific PolySorp, Microfluor 1, Microlite 1+ and Universal Binding (UB) for adsorption of hydrophobic molecules

n

• • Thermo Scientific MaxiSorp is hydrophilic and ideal for antibody sandwich assays

n

• • Thermo Scientific Microfluor 2 and Microlite 2+ are slightly hydrophilic and bind a diverse range of biomolecules

n

nWhite:n

• n
  • Gives maximum reflection

n

• • Minimum autofluorescence and autoluminescence

n

nCompatible with:nnLids for Thermo Scientific MicroWell plates, sealing tape and breathable membrane

Conduct quantitative and qualitative immunoassays, like ELISA and binding assays, with Thermo Scientific™ White 96-Well Immuno Plates. These plates, which are compatible with common instruments, deliver optimal results, lot-to-lot reliability and well-to-well reproducibility.

Features

• • Plate has SBS footprint — fits standard equipment

n

• • Available with flat- or round-bottom wells

n

• • Choice of three surface types for optimal binding

n

• • Certified binding homogeneity/reproducibility

n

nChoice of Surface:n

• n
  • Thermo Scientific PolySorp, Microfluor 1, Microlite 1+ and Universal Binding (UB) for adsorption of hydrophobic molecules

n

• • Thermo Scientific MaxiSorp is hydrophilic and ideal for antibody sandwich assays

n

• • Thermo Scientific Microfluor 2 and Microlite 2+ are slightly hydrophilic and bind a diverse range of biomolecules

n

nWhite:n

• n
  • Gives maximum reflection

n

• • Minimum autofluorescence and autoluminescence

n

nCompatible with:nnLids for Thermo Scientific MicroWell plates, sealing tape and breathable membrane

Conduct quantitative and qualitative immunoassays, like ELISA and binding assays, with Thermo Scientific™ White 96-Well Immuno Plates. These plates, which are compatible with common instruments, deliver optimal results, lot-to-lot reliability and well-to-well reproducibility.

Features

• • Plate has SBS footprint — fits standard equipment

n

• • Available with flat- or round-bottom wells

n

• • Choice of three surface types for optimal binding

n

• • Certified binding homogeneity/reproducibility

n

nChoice of Surface:n

• • Thermo Scientific PolySorp, Microfluor 1, Microlite 1+ and Universal Binding (UB) for adsorption of hydrophobic molecules

n

• • Thermo Scientific MaxiSorp is hydrophilic and ideal for antibody sandwich assays

n

• • Thermo Scientific Microfluor 2 and Microlite 2+ are slightly hydrophilic and bind a diverse range of biomolecules

n

nWhite:n

• • Gives maximum reflection

n

• • Minimum autofluorescence and autoluminescence

n

Compatible with: Lids for Thermo Scientific MicroWell plates, sealing tape and breathable membrane

Thermo Scientific™ Zeba Spin Desalting Columns (7K MWCO) contain a size-exclusion chromatography resin that provides excellent protein desalting performance in a centrifuge format for 2µL to 4mL samples.

nThese specially designed polypropylene centrifuge columns are pre-packed with high-performance Zeba Desalting Resin to facilitate rapid protein desalting with high recovery. The resin effectively traps salts and other small sample components but does not impede proteins and other macromolecules, which pass directly into the centrifuge collection tube. These particular columns use Zeba Resin having a molecular-weight cutoff (MWCO) rating equal to 7K; this ensures that proteins larger than 7000 MW will be recovered with high efficiency.nnThe easy-to-use spin column format dramatically improves results over standard drip-column methodologies. This system eliminates the need to wait for samples to emerge by gravity flow and to monitor fractions for protein recovery. Samples are processed in these desalting columns using a standard centrifuge or microcentrifuge. No chromatography hardware or column preparation steps are required. Multiple samples can be processed in less than 10 minutes. Zeba Spin Desalting Columns are available in five different column sizes, and these provide trouble-free desalting and buffer exchange for sample volumes ranging from 2µL to 4mL

Highlights:

• • Fast centrifuge format – resin and columns are optimized for rapid processing by centrifugation with standard collection tubes and rotors

• • Replaces drip-column methods – no need to collect and screen slowly emerging protein fractions

• • Exceptional protein yields – recover greater than 95% in typical applications with minimal dilution

• • Five convenient sizes – columns with 75µL to 10mL of Zeba Resin provide desalting matched for sample volumes between 2µL and 4mL

• • 7K MWCO – resin is rated to recover proteins larger than 7000 daltons and retain (remove) solutes smaller than 1000 MW

Requires:nnMicrocentrifuge and microcentrifuge collection tubes (for 75µL and 0.5mL columns) or centrifuge and collection tubes (15mL tubes for 2 and 5mL columns; 50mL tubes for 10mL columns)

Cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts. Used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells.

• • Gentle on cells

n

• • Room temperature-stable for 24 months

n

• • Easy to use

n

• • Animal origin-free

n

• • Gentle on cells, purity increases specificity due to the action of a single enzyme and reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts

n

• • Can be directly substituted for trypsin in existing protocols

n

• • Dilution alone inactivates TrypLE Express, avoiding the need for trypsin inhibitors, such as FBS

n

n nnEnergy efficient, less hazardous, sustainable packagingnnCell Culture, Mammalian Cell Culturen

Order Info

Shipping Condition: Room Temperature

Compliance

Manufactured at a cGMP-compliant facility that is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. TrypLE™ Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts.

• • Gentle On Cells

n

• • urity increases specificity due to the action of single enzyme, reducing damage caused by cleavage from enzymes present in some trypsin preparations and in other protease extracts

n

• • Room-Temperature Stable for 24 months

n

• • Ready to use

n

• • Can be directly substituted for trypsin in existing protocols

n

• • Dilution alone inactivates TrypLE Express, avoiding the need for trypsin inhibitors, such as FBS

n

Energy efficient, less hazardous, sustainable packagingnnCell Culture, Mammalian Cell Culture

Due to its digestive strength, trypsin is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements.

• • Documented traceability

n

• • Dual-site cGMP Manufacturing

n

• • Quality testing

n

• • Solutions tested for pH, osmolality, sterility, and performance

n

• • Raw materials are verified for e-beam irradiation and tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis

n

• • Documented traceability available including lot traceability, animal origin certificates, lot analyses, irradiation certificates, viral inactivation summary, and supply chain transparency

n

• • Each drop of beads contains both a positive population that will capture any mouse, rat or hamster antibody and a negative population that will not react with antibody

n

• • This bimodal distribution can be used for single-color compensation controls in multicolor flow cytometry experiments

n

• • Compatible with all fluorochromes excited by an ultraviolet (355nm) or violet (405nm) laser

n

• • Should be used with standard staining buffers which contain PBS or HBSS, protein such as BSA or FBS, and sodium azide. No other additives should be used.

n

• • Binds the spherical particles for use as a single-color compensation controls.

n

• • Binds with mouse, rat and hamster antibodies, but also bind with rabbit and recombinant human antibodies.

n

• • Designed for use in compensation with all fluorochromes excited by ultraviolet (355 nm) violet (405 nm), blue (488 nm), green (532 nm), yellow-green (561 nm), and red (633-640 nm) lasers.

n

• • Compensate in a manner similar to cells when used with Brilliant Violet 785/786-, Brilliant Violet 711-, Super Bright 780- and Super Bright 702-conjugated antibodies.

n

• • Multispecies compatibility—bind with mouse, rat, hamster, rabbit and recombinant human antibodies

n

• • Improved fluorophore compatibility

n

• • Each drop of beads contains two populations: a positive population that bind with any mouse, rat, hamster, rabbit or recombinant human antibody and a negative population that will not bind with antibody.

n

• • When a fluorochrome-conjugated antibody is added to the beads, both positive and negative populations are observed.

n

• • This bimodal distribution can be used for single-color compensation controls to accurately setup instrumentation for multicolor flow cytometry experiments.

n

• • Aqueous solution prepared by dissolving Na₂EDTA•2H₂O in distilled, deionized water and adjusting the pH to 8.0 with sodium hydroxide

n

• • Suitable for biochemistry or molecular biology applications requiring chelator of divalent metal ions

n

Agarose Gel Electrophoresis, ChIP-on-Chip, Chromatin Biology, DNA & RNA Purification & Analysis, DNA Extraction, General gDNA Purification Reagents & Accessories, Genomic DNA Purification, Maxiprep, Midiprep, Miniprep, Nucleic Acid Gel Electrophoresis & Blotting, Plasmid DNA Purification, RNAi, Epigenetics & Non-Coding RNA Research.

Order Info

Shipping Condition: Room Temperature

• • UltraPure DNase/RNase-Free Distilled Water is 0.1-µm membrane-filtered and tested for DNase and RNase activity

n

• • Performance and quality testing: No DNase, RNase, or protease activity detected. Our distilled water system is routinely monitored for compliance with current USP monograph test requirements for Water for Injection (WFI)

n

• • Sustainable packaging

n

The Thermo Scientific™ VisionMate™ HSX High Speed Barcode Reader decodes 2D tube barcodes in as little as one second. Compatible with Matrix™ and Nunc™ tubes in 24, 48, 96, and 384 format racks. The redesigned VisionMate™ Suite software provides a range of export options for seamless transfers of barcode data to your LIMS, ELN, or database. Pre-loaded rack configurations allow you to rapidly read Matrix™ and Nunc™ racks of tubes without additional set up steps. The VisionMate™ HSX can decode both 2D and linear rack barcodes, providing additional tracking options. Customizable LED visual feedback provides the user with instant notification on successful reads, as well as notification of any read errors. The VisionMate™ HSX carries an IP66 rating, which protects the instrument from various adverse environmental conditions in your laboratory and establishes the critical environment barrier needed for use in a clean room. The base unit is fully powered by a single USB connection to your computer. The VisionMate™ HSX can be used on the benchtop or integrated into your automated system.

• Reads 2D barcoded Matrix™ and Nunc™ tubes in 24, 48, 96, and 384 format racks in as little as one second.
• Graphical user interface provides multiple options for data viewing and export to your LIMS, ELN, or database.
• Pre-loaded rack configurations for Matrix™ and Nunc™ racks.
• Can read both 2D and linear rack barcodes for additional tracking options.
• The included magnetic linear barcode reader can be positioned in various locations on the all metal housing of the base unit to read linear rack barcodes.
• Cyroprotection provides resistance to the accumulation of frost on the reading surface.
• Customizable LED feedback gives the user instant notification of successful reads and read errors relating to number of tubes, rack orientation, and rack barcodes.
• Base reader is fully powered by a single USB connection to your computer.
• IP66 Rating provides a critical environment barrier that enables clean room use and protects the reader from adverse environmental conditions.
• Can be used as a benchtop unit or integrated into your automated system.
• A perfect complement to the Thermo Scientific™ Matrix™ and Nunc™ 2D barcoded tube offerings, the VisionMate™ HSX provides valuable process flexibility and compatibility in storage workflows in drug discovery, biological, or genomic storage environments.
• The VisionMate™ HSX is a valuable addition to storage processes which include other Thermo Scientific™ products:
  • Thermo Scientific™ Capit-All™ Tube Capping System
  • Thermo Scientific™ 8-Channel Handheld Screw Cap Decapper
  • Thermo Scientific™ SuperSealer

Vitamin E acetate is used as a fortifying agent and dietary supplement in food. It is used in dermatological products such as skin creams. It is an alternative to tocopherol and provides protection against the sun’s ultraviolet rays.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Vitamin E acetate is used as a fortifying agent and dietary supplement in food. It is used in dermatological products such as skin creams. It is an alternative to tocopherol and provides protection against the sun’s ultraviolet rays.

nThis Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Vitamin E acetate is used as a fortifying agent and dietary supplement in food. It is used in dermatological products such as skin creams. It is an alternative to tocopherol and provides protection against the sun’s ultraviolet rays.

nThis Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Used in conductometric titration, Synthesis of nanomaterials. Mostly it is used as solvent in industries and in all research fields.

nThis Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Used in conductometric titration, Synthesis of nanomaterials. Mostly it is used as solvent in industries and in all research fields.

nThis Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Used in conductometric titration, Synthesis of nanomaterials. Mostly it is used as solvent in industries and in all research fields.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

It is an ultra-filtered supplement and is ideal for mammalian cell culture applications.

Water-soluble extract of autolyzed yeast cells suitable for use in culture media

Rapidly desalt protein and biological samples for manual or automated liquid chromatography (LC) with Thermo Scientific Zeba Spin Desalting columns, plates, and cartridges, which enable =95% retention of salts and other small molecular contaminants (<1,000 Da). Zeba Spin Desalting products contain a proprietary high-performance size-exclusion chromatography resin that enables excellent protein desalting and recovery of 7K molecular weight cutoff (MWCO) molecules using a centrifuge format.

nZeba Spin Desalting columns, plates, and chromatography cartridge fittings are convenient, ready-to-use devices compatible with major automated liquid-chromatography systems or for manual syringe processing. The polypropylene columns have a 7K MWCO and contain a proprietary high-performance size-exclusion chromatography resin (Zeba High-Performance Resin) that offers excellent protein desalting and recovery in a centrifuge format. When using Zeba Spin Desalting Columns, even very dilute (25 µg/mL) protein samples can be successfully processed to obtain greater than 95% retention (removal) of salts and other small molecules (<1000 MW), and good recovery of proteins and other macromolecules (>7000 MW).

Similarly, Zeba Desalting plates (7K MWCO) are polypropylene devices that contain Zeba High-Performance Resin and provide excellent protein desalting performance, with high recovery of proteins >7000 Da in a centrifuge format for small sample volumes. The plates enable fast, consistent, well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations. Zeba 96-well spin desalting plates require no resin dispensing or hydration and provide the same high protein recovery as spin columns with at least 95% removal of salts and other small molecules. One plate can process 96 small sample volumes (20 to 100 µL) in just five minutes, offering rapid and exceptional protein desalting.

The Zeba Desalting Chromatography Cartridges contain Zeba High-Performance Resin for desalting or buffer exchange applications. This resin is ideal for removing low molecular weight compounds including salts, fluorescent dyes, biotin, and other small labeling reagents. Protein samples can be processed with unsurpassed protein recovery and 95% retention of salts and other small molecular contaminants (<1,000 Da). Zeba Desalting cartridges provide fast, easy, and reproducible chromatographic separations and can be regenerated for multiple uses.