Thermo Scientific

Enhance cell culture performance with Gibco™ Difco™ TC Yeastolate Ultra-Filtered (UF) Low Bioburden while also mitigating risk in bioprocessing methods.

Enhance cell culture performance with Gibco™ Difco™ TC Yeastolate Ultra-Filtered (UF) Low Bioburden while also mitigating risk in bioprocessing methods.

Formulation of culture media for cultivation of heterotrophic microorganisms

Difco™ Yeast Extracts are concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells derived from primary grown baker’s yeast.

DYKDDDDK Epitope Tag Synthetic Peptide, PEP-087, from Invitrogen.

nDYKDDDDK Tag (FLAG-tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins. similar epitope as the FLAG tag from SigmaThis peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product (Product &Num; PA1-984B) and fusion proteins containing the epitope tag DYKDDDDK. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (?70-fold) for competitive inhibition of antibody-protein binding reactions.Reconstitute with 0.1 mL of distilled water.

• • Activation of T cells without need for feeder cells

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• • Activated cells that retain in vivo-like function

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• • Maximum reproducibility without contamination by soluble antibodies or mitogens

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• • From 100- to 1,000-fold T cell expansion

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• • Cell Type: T Cells (Whole Population)

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• • Final Product: Cells

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• • Reactivity: Human

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• • Sample Type: Cell Cultures, PBMC

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• • Target Species: Human

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Cell Activation and Expansion, Cell Analysis, Cell Isolation, Cell Isolation and Expansion, Human Cell Activation and Expansion, Human Cell Isolation

• • Activation of T cells without need for feeder cells

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• • Activated cells that retain in vivo-like function

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• • Maximum reproducibility without contamination by soluble antibodies or mitogens

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• • From 100- to 1,000-fold T cell expansion

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• • Cell Type: T Cells (Whole Population)

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• • Final Product: Cells

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• • Reactivity: Human

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• • Sample Type: Cell Cultures, PBMC

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• • Target Species: Human

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Cell Activation and Expansion, Cell Analysis, Cell Isolation, Cell Isolation and Expansion, Human Cell Activation and Expansion, Human Cell Isolation

Order Info

Shipping Condition: Room Temperature

The DynaMag-2 magnet combines a strong magnetic attraction for efficient separations in standard microcentrifuge tubes with a flexible and smart ergonomic design.

• • Optimal working volume: 10–2000µL

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• • Holds up to 16 standard 1.5–2mL microcentrifuge tubes in numbered spaces

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• • Good control and visibility of sample

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• • Top rack can be quickly removed from magnet in base, ready for resuspension, vortexing, rotation, or manual sample shaking

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• • Magnet can be used with all types of Dynabeads for efficient isolation of proteins and nucleic acids

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• • Sample rack can be easily removed from base magnet for resuspension, vortexing, rotation or manual sample shaking

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• • Center pin in rack ensures equal vortexing of all tubes

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• • Rack can also be removed and used to store tubes

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• • 16 numbered sample spaces make magnet for parallel handling of multiple samples

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• • With eight tube positions on each side of rack, easily scale protocols to accommodate 96-well plate reactions

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This magnet is not compatible with the MX4 Mixer. The removable sample rack is also available as a separate item. Purchasing an additional sample rack minimizes the need to move tubes between racks. The rack can be placed on the bench, in the refrigerator, or in the incubator.nnCell Activation and Expansion, Cell Analysis, Cell Isolation, DNA and RNA Purification and Analysis, DNA Extraction, Immunoprecipitation, Magnets for Cell Isolation, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis, Sequence-Specific DNA or RNA Purification, Sequencing Reaction Purification.

E-Gel™ Sample Buffer is formulated specifically for maximum performance on E-Gel EX gels as well as other E-Gel precast gels.

Agarose Gel Electrophoresis, DNA and RNA Purification and Analysis, Nucleic Acid Gel Electrophoresis and Blotting

Order Info

Shipping Condition: Room Temperature

The E-Gel™ 1Kb Plus DNA ladder is composed of 20 highly purified, double-stranded DNA bands spanning 100 base pairs to 12,000 base pairs. Ladder has 12 evenly spaced bands ranging from 1Kb to 12Kb, quick orientation band at 1,650 base pairs that forms distinct doublet with 2Kb band, and seven bands of round sizes below 1Kb. The E-Gel 1Kb Plus DNA Ladder is specifically formulated with minimal salts to perform exceptionally well on E-Gel™ EX pre-cast agarose gels. Unlike some other DNA ladders, E-Gel 1Kb Plus DNA Ladder has been specially designed to have bands of round, easy-to-remember sizes. Logical band layout, and quick identification doublet, allow quick and easy estimates of band size at glance.

• • Round, easy-to-remember band sizes

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• • Broad size range (100bp–12Kb)

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• • Formulated specifically for E-Gel EX gels

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• • Room temperature stable for easy storage

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• • Single ladder fits most needs

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• • Ready-to-use formulation for E-Gel and E-Gel EX gels

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Agarose Gel Electrophoresis, DNA and RNA Purification and Analysis, Nucleic Acid Gel Electrophoresis and Blotting.

Perform sample transfers between virtually any tube, rack, microplate or horizontal gel box quickly and efficiently with the Thermo Scientific™ E1-ClipTip™ Equalizer Pipette. Adjustable tip spacing allows you to set the distance between tips by simply sliding the scale to expand or contract to the desired labware format.The NEW My Pipette™ Creator app enables programming of E1-ClipTip from the comfort of your PCnn nnInnovative ClipTip interlocking tip interface technology.

• • Tip is locked and sealed in place until ejected — no dropped tips that waste valuable samples

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• • Complete seal to secure the sample volume in each tip for enhanced accuracy and precision

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• • Tips are attached and ejected with a light touch — eliminates the frustration of banging tips on the pipette

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My Pipette Creator to revolutionize the way you pipette with a new era of technology

• • New web-based pipetting app powered by Thermo Fisher Cloud

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• • Enables efficient, centralized programming and sharing of protocols between E1-ClipTip pipettes and colleagues

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• • Offers pre-programmed protocols, to download and share, for many of your favorite reagent kits and pipetting protocols

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• • Connect E1-ClipTip pipette to the app via a Bluetooth** or USB connection.

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• • Accelerate collaboration and reproducibility, all from the comfort of your computer.

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E1-ClipTip pipette to help you enjoy pipetting

• • E1-ClipTip is designed for optimum comfort and ease of use: the electronic tip ejection and index finger pipetting action let your thumb relax during pipetting.

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• • Two operating options: Step-based Matrix or Presets with settings for your most common applications.

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• • The Programs function allows you to store 30 your most common Matrix or Presets protocols with a specific name.

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• • Connect your E1-ClipTip pipette to My Pipette Creator app conveniently via Bluetooth**

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• • Five different user profiles offer customized settings for multiple users and protocols

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• • MyPipette™ function to customize your main menu view by organizing the icons in your preferred order or creating shortcuts for your favorite programs and pipetting functions. You can also protect important settings from unintentional editing with a password.

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• • Service and calibration trackers remind you when the pipette needs to be sent for service

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• • Li-ion battery for long lasting performance and fast recharging. Recharging with a charging stand or a plug, you decide.

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• • 2-year warranty with convenient web registration

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E1-ClipTip™ Equalizer: transferring multiple samples between different labware formats

• • Adjustable tip spacing allows you to set the distance between pipette tips for different types of labware simply by sliding a scale to expand or contract the tip spacing to the desired format:
    • • Microcentrifuge tubes to/from 24,-48,-96-and 384- well microplates

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    • • Test tubes to/from deepwell blocks

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    • • Microplates and tubes to agarose gels

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• • Save up to 90% in time and repetitions compared to use of single channel manual pipettes***

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E1-ClipTip pipette and ClipTip tips for every applicationn

• • Choose your E1-ClipTip, volume range from 0.5 µL to 1250µL:n
    • • Single channels

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    • • Multichannels with 8, 12 or 16 channels in 96 and 384 formats

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    • • Adjustable tip spacing multichannels with 6, 8, 12 channels in 96 and 384 formats

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    Wide selection of ClipTip tips:

    • • ClipTip 96-format tips are available in 20, 50, 200, 300, 1000 and 1250 µl

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    • • ClipTip 384-format tips are available in 12.5, 30 and 125 µl

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    • • Several packaging options: filtered, sterile, non-sterile, racked and reload

    Compatible with Thermo Scientific ClipTip pipette tips.

* E1-ClipTip 12.5 and all 384 E1-ClipTip pipettes utilize snap and seal technology.
** Bluetooth models available in following countries/regions: Andorra, Australia, Austria, Belgium, Bulgaria, Canada, China, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hong Kong, Hungary, Iceland, India, Ireland, Italy, Japan, Korea (Rep. of), Latvia, Liechtenstein, Lithuania, Luxembourg, Malta, Monaco, Netherlands, New Zealand, Norway, Poland, Portugal, Romania, San Marino, Singapore, Slovakia, Slovenia, Spain, Sweden, Switzerland, Taiwan, United Kingdom, United States and Vatican. For other countries catalog numbers without ‘BT’ extension should be used.
*** Compared to a manual single channel pipette filling a 96-well microplate from microcentrifuge tubes. See Thermo Scientific Smart Note: Is there an easier and more efficient way to transfer liquids between various labware formats, than using traditional handheld pipettes? 2014.

Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.

Invitrogen™ Molecular biology grade, 0.5 M EDTA, pH 8.0 solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Invitrogen™’s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.

• • Provides all of the benefits of Essential 8 Medium such as minimized variability of components, reliable and robust cultures with a xeno-free, cGMP produced media

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• • Convenient two-component kit (500mL basal medium and 10mL supplement), and when used with vitronectin (VTN-N), provides defined system for feeder-free culture of human PSCs

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• • Flexibility is an added benefit

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Order Info

Approved for shipment at Room Temperature or on Wet Ice

• Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL
• Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine™ transfection reagent
• Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
• Supports growth and transfection of 293 cells in culture formats of less than 1mL in multiwell plates to greater than 10L in disposable bioreactors

• • Supports growth of suspension CHO cultures to densities of over 2 x 107 cells per mL, with >95% cell viability

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• • Uses a transfection-compatible, serum-free suspension culture medium that enables high transfection efficiencies of >90% with ExpiFectamine™ CHO transfection reagent

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• • Supports transient transfection of suspension CHO cells at a density of 6 x 106 cells per mL

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• • Facilitates sustained, high-level expression of high-density transiently transfected cultures, achieving yields of up to 3 g per L of recombinant protein

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• • Supports growth and transfection of CHO cells in culture formats from <1 mL in multi-well plates to >10 L in benchtop bioreactors

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• • Does not contain phenol red

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• • Supports growth of suspension CHO cultures to densities of over 2 x 107 cells per mL, with >95% cell viability

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• • Uses a transfection-compatible, serum-free suspension culture medium that enables high transfection efficiencies of >90% with ExpiFectamine™ CHO transfection reagent

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• • Supports transient transfection of suspension CHO cells at a density of 6 x 106 cells per mL

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• • Facilitates sustained, high-level expression of high-density transiently transfected cultures, achieving yields of up to 3 g per L of recombinant protein

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• • Supports growth and transfection of CHO cells in culture formats from <1 mL in multi-well plates to >10 L in benchtop bioreactors

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• • Does not contain phenol red

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Resistance to Geneticin Selection Antibiotic (G418 Sulfate) is conferred by the E. coli APH(3)–I and APH(3)–II resistance genes.

• • Used as selective antibiotic in concentration range of 100 – 200µg/mL for bacteria, or 200 – 500µg/mL for most mammalian cells

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• • Supplied as 50mg/mL solution in water

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Cell Culture, Cloning, Drosophila S2 Cell Culture, High Five™ Cell Culture, Insect Cell Culture, Mammalian Cell Culture, Selection, Sf9 & Sf21 Cell Culture, Transfection, Transformation

Gibco PBS, pH 7.2, is a balanced salt solution widely used in various cell culture applications to maintain a stable pH and osmotic balance, making it ideal as a wash, transport, and dilution buffer.

• • Originally used with RPMI 1640 and Minimum Essential Medium (MEM), ITS-G solution is now routinely used with other basal media to support a wide variety of cell types in the presence of 2 to 4% FBS

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• • Insulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids

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• • Transferrin is an iron carrier and it may also help to reduce toxic levels of oxygen radicals and peroxide

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• • Selenium, as sodium selenite, is a cofactor for glutathione peroxidase and other proteins, and is used as an antioxidant in media

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Human EGF Recombinant Protein for Western Blot, IHC, ELISA, Ctrl, FN

nEGF has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin.

• • This product is provided as lyophilized and carrier-free

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• • High purity: no interference from other proteins or contaminants

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• • High biological activity: more results with less protein

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• • Proven compatibility: to help ensure growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein

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• • High biological activity: in-house activity testing of recombinant proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, anti-viral, and protease assays; specific activity is determined by the dose dependent proliferation of mouse BALB/3T3 cells

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• • Mercaptoethanol is not stable in solution. so most protocols require daily supplementation

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• • Concentration of 55mM in Dulbecco’s Phosphate Buffered Saline (DPBS)

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• • Manufactured at a cGMP compliant facility, located in Grand Island, New York, registered with the FDA as a medical device manufacturer and certified to ISO 13485 standards

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• • Manufactured at a cGMP-compliant facility located in Grand Island, New York, registered with the FDA as a medical device manufacturer and certified to ISO 13485 standards

Cell Culture, Clinical, Clinical and Translational Research, Cytogenetics, Karyotyping, Mammalian Cell Culture

The most cited neuronal cell culture supplement, B-27 Supplement is an optimized serum-free supplement used to support the low- or high-density growth and short- or long-term viability of embryonic, post-natal, and adult hippocampal and other CNS neurons. B-27 Supplement is provided as a 50X liquid and is intended for use with Neurobasal Medium or Neurobasal-A Medium for neuronal cell culture without the need for an astrocyte feeder layer.

• • Provided as 50X liquid

• • Used with Neurobasal™ Medium for neuronal cell culture

• • Tested in growth assay utilizing rat (Sprague Dawley) embryonic hippocampal neurons

• • Tested also for endotoxin at a 1X concentration

BHI broth is used for the cultivation of a wide variety of microorganisms, including bacteria (streptococci, pneumococci and meningococci), yeasts and molds. It serves as a base for supplemented media containing 0.1% agar, Fildes enrichment or 6.5% sodium chloride.

• To use dehydrated product: suspend 37g in 1L of purified water
• Displays a good cultural response to Neisseria meningitides 13090, Streptococcus pneumonia 6305 and Streptococcus pyogenes 19615
• pH (3.7% solution, 25°C): 7.4 ± 0.2

Certifications

AOAC, BAM, CCAM, CLSI, CMPH2, COMPF, EPA, ISO, MCM9, SMD, SMWW, USDA

• An animal origin (AO) acid hydrolysis of casein
• Less processing results in higher sodium chloride and iron content
• It has successfully be used in the production of a number of animal health vaccines
• Bacto™ Casamino Acids, technical has been shown to support the growth of Bordetella, Haemophilus, and Vibrio species
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

• Bacto Malt Extract is the water-soluble portion of malted barley
• Due to the extraction process, this animal origin-free supplement provides a very high carbohydrate content to any medium
• It has been used successfully in microbial fermentation applications
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

• Bacto Neopeptone is an animal origin (AO) enzymatic digest of bovine (milk) and porcine protein
• This nutritional supplement provides vitamins, nucleotides, minerals, and peptides in a variety of sizes to any culture formulation
• It has been used as a component of culture media in the cultivation of human pathogens, notably Bordetella pertussis and group A streptococci
• Also used with Borrelia burgdorferi and Clostridium species, to produce vaccines for animal health application
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

Applications of Bacto Neopeptone include:

• Recommended for use in media for detection of fungi
• Reported to provide nutrients for support of spirochetes and protozoa
• Microbial fermentation
• Production of vaccines for animal health applications

• Bacto Proteose Peptone No. 3 is a member of the Bacto Proteose Peptones, which are enzymatic digests of protein
• Studies show that no single peptone is the most suitable nitrogen source for every microbiological application
• These findings led to the development of Bacto Proteose Peptone, Bacto Proteose Peptone No. 2, and Bacto Proteose Peptone No. 3. Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone
• Bacto Proteose Peptone No. 3 is used in preparing microbiological and mammalian cell culture media
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

• Bacto Proteose Peptone No. 3 is a member of the Bacto Proteose Peptones, which are enzymatic digests of protein
• Studies show that no single peptone is the most suitable nitrogen source for every microbiological application
• These findings led to the development of Bacto Proteose Peptone, Bacto Proteose Peptone No. 2, and Bacto Proteose Peptone No. 3. Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone
• Bacto Proteose Peptone No. 3 is used in preparing microbiological and mammalian cell culture media
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

• Bacto Proteose Peptone No. 3 is a member of the Bacto Proteose Peptones, which are enzymatic digests of protein
• Studies show that no single peptone is the most suitable nitrogen source for every microbiological application
• These findings led to the development of Bacto Proteose Peptone, Bacto Proteose Peptone No. 2, and Bacto Proteose Peptone No. 3. Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone
• Bacto Proteose Peptone No. 3 is used in preparing microbiological and mammalian cell culture media
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

• Bacto Proteose Peptones are enzymatic digests of protein
• Studies show that no single peptone is the most suitable nitrogen source for every microbiological application
• These findings led to the development of Bacto Proteose Peptone, Bacto Proteose Peptone No. 2, and Bacto Proteose Peptone No. 3. Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone.
• Bacto Proteose Peptone is used in preparing microbiological culture media and in producing bacterial toxins
• Store at 2° to 25°C in cool, dry conditions.

This supplement is a mixture of peptides, amino acids, and carbohydrates, both simple and complex. Customers have successfully used Gibco™ Bacto™ TC Lactalbumin Hydrolysate to support the growth of many mammalian, bacterial, and insect cell types, and it has been used to produce both recombinant proteins and animal health vaccines. Customers have also used this supplement as an amino acid supplement for mammalian cell culture media.

• Bacto Tryptone is an animal origin (AO) pancreatic digest of casein
• It is included in standard methods manual applications and is listed in the Reagent section of the United States Pharmacopeia as meeting the specifications for a pancreatic digest of casein, a component in many of the media listed
• The nutritional profile of this supplement is unique in that it provides a range of nutrients
• Customers have used this supplement in fermentation applications and shown that it successfully supports the growth of many bacterial species during the production of animal health vaccines
• This includes Escherichia coli, and Fusobacterium and Agrobacterium species when used together with Bacto Yeast Extract
• Store in cool, dry conditions in well-sealed containers at 2° to 25°C

Features:

• Pancreatic digest of casein
• Developed while investigating a peptone particularly suitable for the elaboration of indole by bacteria
• Animal origin nutritional supplement

Applications of Bacto Tryptone include:

• Works well in fermentation applications
• Use with common organisms such as Escherichia coli, as well as uncommon organisms such as the diatom Nitzschia laevis

• Bacto Tryptone is an animal origin (AO) pancreatic digest of casein
• It is included in standard methods manual applications and is listed in the Reagent section of the United States Pharmacopeia as meeting the specifications for a pancreatic digest of casein, a component in many of the media listed
• The nutritional profile of this supplement is unique in that it provides a range of nutrients
• Customers have used this supplement in fermentation applications and shown that it successfully supports the growth of many bacterial species during the production of animal health vaccines
• This includes Escherichia coli, and Fusobacterium and Agrobacterium species when used together with Bacto Yeast Extract
• Store in cool, dry conditions in well-sealed containers at 2° to 25°C

Features:

• Pancreatic digest of casein
• Developed while investigating a peptone particularly suitable for the elaboration of indole by bacteria
• Animal origin nutritional supplement

Applications of Bacto Tryptone include:

• Works well in fermentation applications
• Use with common organisms such as Escherichia coli, as well as uncommon organisms such as the diatom Nitzschia laevis

• Bacto Yeast Extracts are concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells, derived from primary grown baker’s yeast
• These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial fermentation, and insect cell culture applications
• Yeast extract provides essential water-soluble vitamins, amino acids, peptides, and carbohydrates to any medium formulation
• Bacto Yeast Extract is one of the most complete and versatile fermentation supplements available
• It is an important ingredient for the microbiological assay of vitamins
• Yeast extract is also of value in the assay of antibiotics
• B factor, a growth substance necessary for the production of rifampin in a Nocardia species, can be isolated from yeast extract
• Bacto Yeast Extract, Technical, was developed to provide products priced for the biotechnology market with acceptable clarity and growth-promoting characteristics
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

Related yeast extract products include:

• Gibco Yeast Extract – Developed to provide a product for the biotechnology/pharmaceutical market with acceptable clarity and growth-promoting characteristics. Media formulations containing yeast extract are specified in standard methods for various applications.
• Difco Yeast Extract UF – Ultra-filtered and specifically designed for mammalian cell culture applications. With its low endotoxin level and high content of naturally occurring B vitamins, it can be used effectively to reduce the need for fetal bovine serum. It has an endotoxin level of less than or equal to 500 EU/g
• Difco Yeast Extract Low-Dusting (LD) – Designed to limit the amount of dust generated compared to other spray-dried yeast extracts.

• Bacto Yeast Extracts are concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells, derived from primary grown baker’s yeast
• These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial fermentation, and insect cell culture applications
• Yeast extract provides essential water-soluble vitamins, amino acids, peptides, and carbohydrates to any medium formulation
• Bacto Yeast Extract is one of the most complete and versatile fermentation supplements available
• It is an important ingredient for the microbiological assay of vitamins
• Yeast extract is also of value in the assay of antibiotics
• B factor, a growth substance necessary for the production of rifampin in a Nocardia species, can be isolated from yeast extract
• Bacto Yeast Extract, Technical, was developed to provide products priced for the biotechnology market with acceptable clarity and growth-promoting characteristics
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

Related yeast extract products include:

• Gibco Yeast Extract – Developed to provide a product for the biotechnology/pharmaceutical market with acceptable clarity and growth-promoting characteristics. Media formulations containing yeast extract are specified in standard methods for various applications.
• Difco Yeast Extract UF – Ultra-filtered and specifically designed for mammalian cell culture applications. With its low endotoxin level and high content of naturally occurring B vitamins, it can be used effectively to reduce the need for fetal bovine serum. It has an endotoxin level of less than or equal to 500 EU/g
• Difco Yeast Extract Low-Dusting (LD) – Designed to limit the amount of dust generated compared to other spray-dried yeast extracts.

• Bacto Yeast Extracts are concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells, derived from primary grown baker’s yeast
• These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial fermentation, and insect cell culture applications
• Yeast extract provides essential water-soluble vitamins, amino acids, peptides, and carbohydrates to any medium formulation
• Bacto Yeast Extract is one of the most complete and versatile fermentation supplements available
• It is an important ingredient for the microbiological assay of vitamins
• Yeast extract is also of value in the assay of antibiotics
• B factor, a growth substance necessary for the production of rifampin in a Nocardia species, can be isolated from yeast extract
• Bacto Yeast Extract, Technical, was developed to provide products priced for the biotechnology market with acceptable clarity and growth-promoting characteristics
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

Related yeast extract products include:

• Gibco Yeast Extract – Developed to provide a product for the biotechnology/pharmaceutical market with acceptable clarity and growth-promoting characteristics. Media formulations containing yeast extract are specified in standard methods for various applications.
• Difco Yeast Extract UF – Ultra-filtered and specifically designed for mammalian cell culture applications. With its low endotoxin level and high content of naturally occurring B vitamins, it can be used effectively to reduce the need for fetal bovine serum. It has an endotoxin level of less than or equal to 500 EU/g
• Difco Yeast Extract Low-Dusting (LD) – Designed to limit the amount of dust generated compared to other spray-dried yeast extracts

• Concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells derived from primary grown baker’s yeast
• These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial fermentation, and insect cell culture applications
• Yeast extract provides essential water-soluble vitamins, amino acids, peptides, and carbohydrates to any medium formulation
• Bacto Yeast Extract is an important ingredient for the microbiological assay of vitamins
• B factor, a growth substance necessary for the production of rifampin in a Nocardia species, can be isolated from yeast extract
• Developed to provide products priced for the biotechnology market with acceptable clarity and growth-promoting characteristics
• Store in cool, dry conditions in well-sealed containers at 2°C to 25°C

The combination of pancreatic digest of casein and yeast extract provides synergistic nutritional benefits that are not provided by the components alone, resulting in a unique nutritional profile. Customers have shown the combined use of these two peptones, when supplied as Biosate Peptone, improves toxin production with Clostridial species for vaccines, such as Clostridium botulinum and Clostridium perfringens.

Gibco™ BiTek™ Fermentation Broth is a bacterial fermentation formulated with ingredients that exhibit superior consistency, performance, and solubility.

Developed while investigating a peptone particularly suitable for the elaboration of indole by bacteria. The final product goes through fewer refinement steps during processing than Gibco™ Bacto™ Tryptone.

Promotes an acceleration in the monoclonal antibody secretion rates of cells in a batch mode for performance in hollow fiber and stir tank systems.

• Suitable for use in perfusion systems
• Shown to increase purified monoclonal antibody yields by 5 to 25 times over classical basal media with similar supplementation
• Reduce media consumption and labor cost, reduce requirements for media storage and preparation time, decrease labor required to perform continuous feeding and decrease contamination because of decreased handling of the culture
• Concentration step is eliminated in most cases, and final bulk harvest is reduced, producing less material to run through the purification process
• Only minimal adaptation is needed
• Hybridomas grown in MAb Basal Medium can be supplemented in the same way as those grown in traditional basal media
• MAb Serum-Free and Animal-Free are complete media and do not require serum supplementation; Basal Medium does require serum supplementation

GIBCO OpTmizer™ CTS™ T-Cell Expansion SFM has been developed for the growth and expansion of human T lymphocytes. OpTmizer™ CTS™ T-Cell Expansion medium is a complete serum-free, xeno-free 1X medium consisting of OpTmizer™ T-Cell Expansion Basal Medium (1 L bottle) and OpTmizer™ T-Cell Expansion Supplement (26 mL), which are mixed together prior to use.

• • Maintains similar phenotype and function (e.g., cytokine secretion profile) of polyclonally activated and cultured T cells as observed for conventional serum supplemented medium

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• • Consistent performance in supporting T cell expansion

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• • Supports high density T cell culture (e.g., >3×10⁶ CD3+ T cells/mL) in static culture
    
    With OpTmizer™ T-Cell Expansion SFM, we offer both basic and clinical research communities a new tool to enhance their success in growing human T-cells. It is specially formulated to allow for superior cell growth & viability, less variability and more consistent results, and easier transition from research to clinical applications.
    
    Workflow benefits:

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• • Minimizing the need to handle purchasing, storage, qualification of serum and thus reducing variability and costs

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• • More rapid T cell expansion, thus reducing cycle time and improving productivity

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• • Complete serum-free, xeno-free medium, allowing for a seamless transition from research to clinical applications and minimizing regulatory workload
    
    “This reagent is classified as a medical device in several countries. If it is not regulated as a medical device in specific region, it should be used for laboratory use only.”

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• Prepared according to the method described by Mueller and Miller
• Supplemented with growth factors, cystine, maltose and iron and inorganic salts
• Successful in fermentation for Pneumococcal, Diptheria, Pertussis, Tetanus and Zoster vaccines
• Support the growth of Bordetella, Corynebacterium, Pseudomonas, Haemophilus and Escherichia
• Dehydrated format with Peptones / Hydrolysates
• DNR (Latex) free