Thermo Scientific

ELISA

ELISA

Human IL-1RA ELISA Kit

Human IL-1α ELISA Kit

Human IL-1β ELISA Kit

Human IL-2 ELISA Kit

Human IL-3 ELISA Kit

Human IL-31 ELISA Kit

Human IL-4 ELISA Kit

Human IL-4 ELISA Kit

Human IL-5 ELISA Kit

Human IL-6 ELISA Kit

Human IL-7 ELISA Kit

Human IL-8 (CXCL8) ELISA Kit

Human IL-9 ELISA Kit

ELISA

ITI-HC1 Synthetic Peptide, PEP-301, from Invitrogen™.

nThe inter-alpha-trypsin inhibitors (ITI) are a family of structurally related plasma serine protease inhibitors involved in extracellular matrix stabilization and in prevention of tumor metastasis. The ITI family contains multiple proteins made up of a light chain (see MIM 176870) and a variable number of heavy chains (Salier et al., 1987 [PubMed 2446322]; Himmelfarb et al., 2004 [PubMed 14744536]). This peptide corresponds to amino acids Q(383) VQESLPELSN (393) of human ITI-HC1 and can be used as a blocking peptide with polyclonal antibody PA1-1755.

Human MCP-1 (CCL2) ELISA Kit

Human MCP-2 (CCL8) ELISA Kit

ELISA

Human MIG (CXCL9) ELISA Kit

Human Neuroserpin ELISA Kit

ELISA

Recombinant Protein

Recombinant Protein

Recombinant Protein

Recombinant Protein

Recombinant Protein

Recombinant Protein

S100A11 (human) Recombinant Protein, RP-75720, from Invitrogen.

nThis product contains purified recombinant human S100A11 protein with an N-terminal hexahistidine tag. This product may be used as a positive control in many immunological assays.

Human sCD40 Ligand ELISA Kit

Human SCF ELISA Kit

Human TIMP-1 ELISA Kit

Human TL-1A ELISA Kit

Human TNF-α ELISA Kit

Human TPO ELISA Kit

ELISA

Human VEGF 165 ELISA Kit

ELISA

Human/Murine/Rat BMP-2 ELISA Kit

Product No. 31806 has been successfully used in Western blot, IF, ICC, IHC and ISH applications. Product No. 31806 reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins. This antibody cross-reacts with mouse IgM (50%) hamster IgG, rat IgG (12%) rat IgM (6%) dog IgG, guinea pig IgG, swine IgG (3%) Rhesus monkey IgG (1%). It has minimal cross-reactivity with immunoglobulins from other species. Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.

nThermo Scientific Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Product No. 31181 has been successfully used in Western blot, IF, ICC, IHC, IP and ISH applications. Product No. 31181 reacts with heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins. Based on solid-phase enzyme immunoassay, this antibody cross-reacts with rat IgG and mouse IgM (25%), hamster IgG (12%), guinea pig IgG and rat IgM (6%), bovine IgM, dog IgM, goat IgM, human IgM, Rhesus monkey IgG, sheep IgG, sheep IgM, swine IgG (1%). It has minimum cross-reactivity with immunoglobulins from other species. The activity measured by a solid phase binding assay is 94%. Store lyophilized product at 4°C until opened. To extend the shelf-life of this product, freeze undiluted product in small aliquots. Avoid repeated freezing and thawing.

nThermo Scientific Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration. Product No. 31172 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications. Product No. 31172 reacts with the heavy chain portion of mouse IgM, but does not react against IgG or non-immunoglobulin serum proteins. However, this antibody may cross-react with IgM from other species. Store product at 4°C until opened. After opening, dilute only enough antibody for a single day’s use. Store remainder at 4°C under sterile conditions. Country of Origin: USA

nThermo Scientific Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Provides complete collection of validated positive controls for the TrueGuide Synthetic gRNA product line to help maximize editing performance. This positive control targeting AAVS1 targets intronic region of the AAVS1 locus and has achieved editing efficiencies of up to 60% in variety of human cell lines. AAVS1 controls easily determine the optimal conditions for delivering CRISPR/Cas9 tools to cell type, allowing to move forward in editing experiments and minimize downstream effort to isolate and validate your edited clones.

AA Sequence of recombinant protein: MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human FGF-acidic. Anti-Human FGF-acidic-specific antibody was purified by affinity chromatography employing an immobilized Human FGF-acidic matrix.

Sandwich ELISA: To detect Human FGF-acidic by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human FGF-acidic (500-P17Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human FGF-acidic.

Western Blot: To detect Human FGF-acidic by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human FGF-acidic is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Beschreibung

BODIPY TR ceramide can be used to stain Golgi apparatus in live cells.

Cell Analysis, Cell Structure, Golgi

Bestellinformation

Shipping Condition: Room Temperature

• • Contain a proprietary fluorescent nucleic acid stain (excitation: 490nm, emission: 520nm) that can be viewed with blue light, which minimizes DNA damage and allows detection down to 1ng/band of DNA

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• • Complete separation (by molecular weight) in 10 minutes

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• • Live monitoring of DNA migration without the hazards of UV light

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• • Easy access to the gel for extraction of DNA bands or transfer to membranes for Southern blotting

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• • Demonstrate over five-fold greater sensitivity than comparable gels containing ethidium bromide allows less sample use per run

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• • Fast analysis of RNA samples to check on their integrity before proceeding with downstream application

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• • The gel cassette is designed to open with a Gel Knife, so that the gel inside can be accessed readily for excision of specific bands or transferred to a membrane for Southern blot analysis

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• • Single comb well format

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• • Sample Type: Double-Stranded DNA (dsDNA), Single-Stranded RNA

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• • Sample Volume; 20µL

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Agarose Gel Electrophoresis, DNA and RNA Purification and Analysis, Nucleic Acid Gel Electrophoresis and Blotting

Order Info

Shipping Condition: Room Temperature

The Gibco LV- MAX Transfection Kit is an essential component of the LV-MAX Lentiviral Production System. It is formulated for the transient transfection of Gibco Viral Production Cells (high-density suspension-adapted human embryonic kidney (HEK) 293-derived cells). The included high-efficiency, cationic, lipid-based transfection reagent, lentiviral production supplement, and LV enhancer are designed to work together to produce the highest possible level of lentiviral particles from the Viral Production Cells when cultured in LV-MAX Production Medium.

• • Designed specifically for transfection of high-density suspension cells to maximize lentiviral production

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• • Enables sustained, high-level production of high-density transiently-transfected cultures, achieving titer of up to 1 x 10⁸ TU/mL (unconcentrated LVVs-GFP)

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• • Reproducible and scalable transfection results, giving users greater confidence in their results

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• • Easy, robust culture and transfection protocols—fits into any transient production workflows.The kit includes three key components required to optimize your lentiviral production. These are only available in the kit and are not sold separately.

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• • LV-MAX transfection reagent—a high-efficiency, cationic, lipid-based transfection reagent uniquely designed for co-transfecting multiple plasmids into high-density Viral Production Cell cultures with high transfection efficiency and low toxicity

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• • LV-MAX supplement—an optimized, chemically defined, serum-free, protein-free, animal origin-free formulation designed to suppress cell growth during transfection and increase lentiviral vector production without compromising the cell viability and interfering with the lentiviral production process

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• • LV-MAX enhancer—a proprietary, animal origin-free formulation designed to boost cell lentiviral vector production

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Molecular biology grade, Invitrogen 1M MgCl₂ solution is supplied in one bottle containing 100mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Invitrogen’s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.n

Order Info

Shipping Condition: Room temperature