Thermo Scientific

• • Offers same thin-wall construction for heat transfer from thermal block to sample

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• • Validated with Applied BiosystemsTM PCR thermal cyclers for optimal fit and performance

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• • Easy-to-tear dual side tabs for simple separation into three, individual 32-well plates

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• • Semi-skirted for easy handling and labeling

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• • Certified human DNA-, DNase- and RNase-free

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• • Standard 250µL working volume, with 300µL maximum well volume when used with adhesive film

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• • Alphanumeric labeling on each 32-well plate for easy well identification

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• • 32-well adhesive seal (MicroAmpTM 32-Well Clear Adhesive Film) available for efficient plate sealing

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Alexa Fluor™ 647 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor™ dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor™ 647 Streptavidin Conjugates:
• Alexa Fluor™ 647 streptavidin conjugate has Ex/Em maxima of ~ (650/668)
• Bright, photostable fluorescence
• High solubility in aqueous solutions
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor™ Dyes
Alexa Fluor™ dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

The Applied Biosystems™ Mouse GAPD (GAPDH) Endogenous Control (VIC™/MGB Probe, Primer Limited) is intended as an endogenous control. It allows relative gene expression quantification in cDNA samples when used with other gene expression assays. Probe is labeled with VIC™ dye – MGB and the primers are limited. Can be used for multiplex or singleplex PCR reactions. Endogenous control is to be used with Inventoried and Non-Inventoried TaqMan™ Gene Expression Assays, TaqMan™ Custom Gene Expression Assays, and/or Custom TaqMan™ Primers and Probes.

Assay Details:
Gene Symbol: GAPDH
RefSeq: NM_008084.2
Probe Exon Location: 3
Amplicon Size: 107
Corresponding TaqMan Assay ID: Mm99999915_g1

TaqMan™ Endogenous Controls
Eliminate months of assay design, formulation, and testing by using TaqMan™ Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction.

Complete Solution for Quantitative Gene Expression
Having a hard time deciding what controls to use to quantify gene expression — even with detailed information on biological systems? Now, with TaqMan™ Endogenous Controls, you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Simple to Use
All components of the TaqMan™ Endogenous Controls are QC tested, formulated into a single 20X mix, and functionally tested. The controls are not only simple to use, but they are also fully compatible with universal conditions for two-step RT-PCR. Just add TaqMan™ Universal PCR Master Mix (with or without AmpErase™ UNG) and your cDNA sample to generate sensitive, reproducible, and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT, 7300, 7500 Real-Time PCR Systems, and the 7000 and 7700 Sequence Detection Systems.

Flexible Offering
We build each endogenous control using our proven 5′ nuclease chemistry. For maximum flexibility, you can choose between two different reporter dyes and two quenchers:

• • A FAM™ dye-labeled TaqMan™ MGB probe (250nM, final concentration) and two unlabeled PCR primers (900nM each)

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• • A VIC™ dye-labeled TaqMan™ MGB probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)

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• • A VIC™ dye-labeled TAMRA™ probe (250nM, final concentration) and two unlabeled PCR primers (150nM each y primer limited)
    
    Choosing the Right Endogenous Control
    Endogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells. For best results, verify that the endogenous control is consistently expressed in the sample set to be tested. Endogenous control expression must be uniform across all samples in the study. For multiplexing, ensure that the gene expression level of the endogenous control is greater than that of the target.
    
    Multiplex vs. Singleplex PCR
    All TaqMan™ Endogenous Controls that contain probes labeled with the VIC™ reporter dye are primer limited. This allows multiplexing of TaqMan™ Endogenous Controls with target gene expression assays, provided that the control gene is more abundantly expressed than the target gene. All TaqMan™ Endogenous Controls that contain probes labeled with the FAM™ reporter dye are not primer limited and are not intended for multiplexing .
    
    For Research Use Only. Not for use in diagnostic procedures.

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Guarantee excellent dispensing results and day-to-day performance with Thermo Scientific™ Multidrop™ Combi, 384 and DW Reagent Dispenser Accessories.

Guarantee excellent dispensing results and day-to-day performance with Thermo Scientific™ Multidrop™ Combi, 384 and DW Reagent Dispenser Accessories.

Guarantee excellent dispensing results and day-to-day performance with Thermo Scientific™ Multidrop™ Combi, 384 and DW Reagent Dispenser Accessories.

Murine G-CSF ELISA Kit

PeproTech’s Murine IGF-I ELISA development kit contains the key components required for the quantitative measurement of natural and/or recombinant murine IGF-I in a sandwich ELISA format.

Murine IL-10 ELISA Kit

ELISA

Murine IL-15 ELISA Kit

Murine IL-3 ELISA Kit

Murine IL-4 ELISA Kit

PeproTech’s Murine IL-5 ELISA development kit contains the key components required for the quantitative measurement of natural and/or recombinant murine IL-5 in a sandwich ELISA format.

Murine IL-6 ELISA Kit

Murine IP-10 (CXCL10) ELISA Kit

ELISA

Murine MIP-1α (CCL3) ELISA Kit

Murine MIP-1β (CCL4) ELISA Kit

PeproTech’s Murine sRANKL ELISA development kit contains the key components required for the quantitative measurement of natural and/or recombinant murine sRANKL in a sandwich ELISA format.

Murine TNF-α ELISA Kit

Murine TNF-α ELISA Kit

Murine VEGF 165 ELISA Kit

Recombinant Protein

Recombinant Protein

Recombinant Protein

Recombinant Protein

Recombinant Protein

Recombinant Protein

Increase filtration efficiency. Thermo Scientific™ Nalgene™ Rapid-Flow™ single use bottle tops feature the exclusive Rapid-Flow support column design, providing fast flow rates and high throughput. The last line of defense against cell culture contamination—Nalgene Rapid-Flow bottle tops are the best choice for the filtration of cell culture media, serum, additives and buffers.

• • Available in PES and SFCA membranes. Devices are color coded according to membrane.

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• • Devices feature the fastest flow rate and lowest clogging of comparable alternatives

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• • 0.1um PES available for mycoplasma protection

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• • 0.2um PES devices are stem cell and mouse embryonic assay (MEA) tested

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• • Non-pyrogenic and endotoxin free – Rapid-Flow filters meet the most rigorous testing standards for any vacuum filtration unit

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• • Available with Fit for Purpose Sterile documentation

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• • Animal origin-free, serum-free and designed specifically for use with the Neon Transfection System

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• • Used for transfection volumes of 10µL, containing 5 x 10⁴-2 x 10⁵ adherent cells or 1 x 10⁶-5 x 10⁵ suspension cells

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• • Cells that have been transfected using included 10µL Neon Tips are ready to be washed and plated into multiwell culture dish

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Adenoviral shRNA, Cell Culture, Lentiviral shRNA & miRNA, Plasmid Transfection, RNAi, RNAi Transfection, RNAi, Epigenetics & Non-Coding RNA Research, Retroviral RNAi, Stem Cell & Primary Cell Transfections, Synthetic siRNA Transfection, Transfection, Vector-Based RNAi, siRNA

Order Info

Shipping Condition: Room Temperature

• • Allows for both long and short term maintenance of homogeneous populations of neuronal cells without the need of an astrocyte feeder layer

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• • Supplemented with 0.5mM L-glutamine or GlutaMAX™-I Supplement and either a serum-free supplement (such as B-27™ Supplement or N-2 Supplement), or with serum

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• • Tested for pH, osmolality, endotoxin, and the absence of bacterial and fungal contamination

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NeutrAvidin Protein, FITC conjugate, 31006, from Invitrogen., 31006, from Invitrogen. Species Reactivity: ; Applications: ELISA, Immunohistochemistry, In Situ Hybridization (ISH), Western Blot.

nNeutrAvidin Protein is deglycosylated native avidin from egg whites. Removal of the excess carbohydrate by an exclusive process yields a protein with a more neutral isoelectric point and less nonspecific binding properties. Purified and conjugated forms of NeutrAvidin Protein provide exceptional performance in Western blot, ELISA and IHC applications that require biotin-binding probes. Assay specificity, sensitivity, and signal-to-noise ratios with NeutrAvidin Protein are generally equivalent or better than with the significantly more expensive streptavidin. Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles and amphibia. The biotin-binding protein contains four identical subunits having a combined mass of 67,000-68,000 daltons. Removing the glycosylation from avidin yields NeutrAvidin Protein with a mass of 60,000 daltons. Carbohydrate-based lectin-binding is reduced to undetectable levels, yet biotin-binding affinity is retained. NeutrAvidin Protein offers the advantages of a neutral pI to minimize nonspecific adsorption, along with lysine residues that remain available for derivatization or other customized conjugation. NeutrAvidin Protein yields the lowest nonspecific binding among the known biotin binding proteins. The specific activity for biotin-binding is approximately 14ug/mg of protein, which is near the theoretical maximum activity.

Normal Porcine Serum Control, 31890, from Invitrogen., 31890, from Invitrogen. Species Reactivity: Not Applicable; Applications: Blocking Assay, Control, Immunocytochemistry, Immunofluorescence, Immunohistochemistry.

nNormal Sera are non-immune serum samples from goat, human, mouse, rabbit and other species that contain the usual complement of serum proteins, including the various classes of immunoglobulins that exist in healthy animals of the particular species. Normal serum provides a sufficient quantity of endogenous proteins to saturate and block nonspecific binding sites on fixed samples or assay substrates, making it useful for immunodetection methods, especially those involving tissue samples such as immunohistochemistry (IHC). Normal sera are also useful as controls for testing general and specific antibody purification methods. When used as an antibody diluent, non-immune serum also supplies a native chemical environment for antibodies to function properly without adding foreign protein components.

Color: White; Sterility: Sterile; Lid: With Lid

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nEnsure consistent and reliable results using the high quality Thermo Scientific™ Nunc™ F96 MicroWell™ White Polystyrene Plate. White plates are optimized for robotics, plate readers and liquid handling systems and designed for key imaging characteristics.nn n

• Background, autoflorescence and crosstalk are improved when you use Nunc Black or White microplates

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• Black microplates are recommended for florescence measurements; with minimum back-scattered light and background fluorescence

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• White microplates are recommended for luminescence measurements; with maximum reflection and minimal autoluminescence

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• Working volume range: 50 to 250µL/well

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• Choice of Thermo Scientific™ Nunclon™ Delta surface for cell-based assays

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• Customized bar coding is available

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Enhance cell attachment, growth and differentiation with Thermo Scientific™ Nunc™ Cell Culture Treated EasYFlasks™. These polystyrene flasks receive the proprietary Nunclon™ Delta surface treatment to maximize adhesion for a broad range of cell types. The ergonomic design with angled neck and wide opening allows easy access to the growth surface by pipettes or cell scrapers.

nFeatures:n

• • Ergonomic design with a 1/3 turn to open and close the cap

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• • Low profile to maximize incubator space

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• • Volume graduations printed and molded on both sides

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• • Slightly angled side walls for complete viewing under a microscope

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• • Sterilized by gamma irradiation and certified to be nonpyrogenic

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• • Nunclon Delta Certification, including testing on four different cell types, is provided in every case.

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nFilter Caps:n

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  • Filter membrane provides effective barrier against micro-organisms.

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• • Well-defined, hydrophobic filter membrane offers continuous venting with consistent gas exchange and no lot-to-lot variation.

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• • Chemically robust membrane meets USP class VI standards and is produced without animal-derived substances.

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nSolid Caps:n

• • Solid caps feature a “Y” mark that allows visual verification of vent position.

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• • Any of the legs in the “Y” pointing upwards indicates that the cap is in venting position.

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• • Vent position can be easily distinguished when flasks are stacked in the incubator.

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Thermo Scientific™ Nunc MicroWell 96-Well Optical-Bottom Plates with Polymer Base have a solid polystyrene black or white upper structures with a polystyrene film at the bottom of the plate. The plates are optimized for fluorescence and luminescence in cell culture and microscopic applications.nn nnWhite or black polystyrene upper structure with polymer or coverglass bottom plates combine the optical clarity of virgin crystalline polystyrene with optimal surface for a wide range of HTS applications.

• • Background, autoflorescence and crosstalk are improved when you use Nunc Black or White microplates

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• • Black microplates are recommended for florescence measurements; with minimum back-scattered light and background fluorescence

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• • White microplates are recommended for luminescence measurements; with maximum reflection and minimal autoluminescence

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• • Available in black for fluorescence assays or white for luminescence and fluorescence assays; both with clear bottoms

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• • Polymer base plate

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• • Working range, 50-200µL/well

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• • Customized bar coding is available

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nOrdering Information:nnPart No. 152040, 152036, 152028 and 152037 is produced in a clean environment.

NuPAGE MOPS SDS Running Buffer (20X) is formulated for running proteins on NuPAGE Novex Bis-Tris gels only. NuPAGE™ MOPS SDS Running Buffer is recommended for separating small- to medium-sized proteins.

• • Get consistent, convenient, high-quality results

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• • Pre-mixed NuPAGE buffers are convenient way to ensure high-quality, consistent electrophoresis results

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Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis

Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important.

NuPAGE MES SDS Running Buffer (20X) is formulated for running proteins on NuPAGE Novex Bis-Tris gels only. NuPAGE MES SDS Running Buffer is recommended for separating small- to medium-sized proteins.

• • Use of MES buffer allows proteins to run faster

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• • Ensures high-quality, consistent electrophoresis results

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Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis

NuPAGE Sample Reducing Agent contains 500mM dithiothreitol (DTT) at a ready-to-use, 10X concentration in a stabilized liquid form. Use NuPAGE Sample Reducing Agent when running proteins on NuPAGE Novex Bis-Tris gels to maintain protein samples in a reduced state during electrophoresis.nn1D Gel Electrophoresis, 2D Gel Electrophoresis, IsoElectric Focusing, Protein Gel Electrophoresis, Protein Sample Fractionation, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis

NuPAGE Sample Reducing Agent contains 500mM dithiothreitol (DTT) at a ready-to-use, 10X concentration in a stabilized liquid form. Use NuPAGE Sample Reducing Agent when running proteins on NuPAGE Novex Bis-Tris gels to maintain protein samples in a reduced state during electrophoresis.nn1D Gel Electrophoresis, 2D Gel Electrophoresis, IsoElectric Focusing, Protein Gel Electrophoresis, Protein Sample Fractionation, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis

• • NuPAGE Novex Tris-Acetate gels provide excellent separation of large molecular weight proteins when used with NuPAGE Tris-Acetate SDS Running Buffer

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• • Premixed buffers are a convenient way to ensure high-quality, consistent electrophoresis results

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Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis

Opti-MEMI medium is recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent.

• • Opti-MEM is modified as follows: with L-glutamine; without phenol red

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• • Can be used with a variety of suspension and adherent mammalian cells

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• • Sustainable packaging

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Cell Culture, Mammalian Cell Culture, Plasmid Transfection, RNAi Transfection, Synthetic siRNA Transfection, Transfection, shRNA and miR RNAi Plasmid Transfection.

Take accurate, reproducible ion concentration measurements by calibrating your Ion Selective Electrode with high-quality Thermo Scientific™ Orion™ ISE Calibration Standards. Use these standards with electrodes including ammonia, chloride, fluoride, nitrate, potassium, silver, sodium and sulfide. Orion calibration standards are NIST traceable and manufactured under ISO 9001 quality standards.

Murine IL-1β ELISA Kit

Description

nPierce Protease Inhibitor Tablets protect proteins from proteolytic degradation through inhibition of the serine-proteases, cysteine-proteases, aspartic acid-proteases and aminopeptidases that are typically present in cellular lysate samples. The tablets contain AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A. Stable for storage at 4°C for up to 12 months, each mini or large tablet tablet is sufficient for 10mL or 50mL of solution, respectively, and is suitable for reagent preparation prior to protein extraction from tissue and cultured cells.

Highlights:

• • Improved formulation&mdsashtablets dissolve quickly into a clear solution

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• • Economical – more cost-effective than other commercially available formulations for equivalent volumes

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• • Convenient&mdsashready-to-use, fully disclosed, broad-spectrum formulations

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• • Compatible&mdsashuse directly with Thermo Scientific™ Pierce™ BCA Assays

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• • EDTA-free- formulated without EDTA, a metalloproteinase inhibitor (only for EDTA-free formulations)

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Thermo Scientific™ Pierce Trifluoroacetic Acid (TFA) is manufactured and tested to meet strict specifications that ensure superior performance for use as an ion-pairing agent in reverse-phase peptide separations.

nFor complex peptide separations, the key to success can be to vary selectivity. Varying mobile phase composition on the same column can change selectivity enough to resolve peptide that would otherwise overlap. The TFA concentration is usually specified as 0.1% for reverse-phase HPLC of peptides. For reproducible separations from run-to-run or from lab-to-lab, it is essential to make concentrations the same.nnHighlights:n

• • Purity: >99.5% TFA and exceptional clarity allows sensitive, nondestructive peptide detection at low UV wavelengths

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• • High-performance packaging: Packaged under nitrogen in amber glass with protective TFE-lined fluorocarbon caps to ensure TFA integrity

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• • Choice of formats for convenience: 1mL ampules can prepare 1L of 0.1% v/v TFA solution for the mobile phase in reverse-phase chromatography in moments

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Ordering Information: X in the ordering table indicates that hazardous shipping charges apply.

1 L, 10X, Tris-Glycine SDS Buffer